Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticles
In tissue repair, cell behaviors can be modulated by sensing and responding to the topographical and biochemical cues provided by the surrounding microenvironment. Herein, we designed and fabricated a class of uniaxially aligned nanofiber yarns welded with electrosprayed microparticles to guide the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Materials Today Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590049823000036 |
| _version_ | 1811160835110731776 |
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| author | Xiaopei Zhang Mingxia Guo Qingxia Guo Na Liu Yuanfei Wang Tong Wu |
| author_facet | Xiaopei Zhang Mingxia Guo Qingxia Guo Na Liu Yuanfei Wang Tong Wu |
| author_sort | Xiaopei Zhang |
| collection | DOAJ |
| description | In tissue repair, cell behaviors can be modulated by sensing and responding to the topographical and biochemical cues provided by the surrounding microenvironment. Herein, we designed and fabricated a class of uniaxially aligned nanofiber yarns welded with electrosprayed microparticles to guide the directional growth of axons and the migration of neural stem cells. By tuning the depositing density of microparticles on the nanofiber yarns, the surface roughness was varied, which facilitated the directional axon outgrowth of PC12 and SH-SY5Y cells and the alignment of Schwann cells. The microparticles were further modified by loading nerve growth factors in the core and then deposited on the nanofiber yarns. Owing to the synthetic effects provided by the physical and biological signals, the migration of neural stem cells was significantly accelerated. Taken together, the uniaxially aligned nanofiber yarns welded by functionalized microparticles show promising use in manipulating axonal growth and the migration of neural stem cells, which will provide a novel approach to clinical repair of nerve injuries. |
| first_indexed | 2024-04-10T06:05:03Z |
| format | Article |
| id | doaj.art-cb74debb74064c1d9b19c21fc405fa7f |
| institution | Directory Open Access Journal |
| issn | 2590-0498 |
| language | English |
| last_indexed | 2024-04-10T06:05:03Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Advances |
| spelling | doaj.art-cb74debb74064c1d9b19c21fc405fa7f2023-03-03T04:25:13ZengElsevierMaterials Today Advances2590-04982023-03-0117100343Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticlesXiaopei Zhang0Mingxia Guo1Qingxia Guo2Na Liu3Yuanfei Wang4Tong Wu5Institute of Neuroregeneration and Neurorehabilitation, Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaInstitute of Neuroregeneration and Neurorehabilitation, Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaInstitute of Neuroregeneration and Neurorehabilitation, Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaInstitute of Neuroregeneration and Neurorehabilitation, Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaCentral Laboratory, Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, 266001, ChinaInstitute of Neuroregeneration and Neurorehabilitation, Qingdao Medical College, Qingdao University, Qingdao, 266071, China; Shandong Key Laboratory of Medical and Health Textile Materials, Collaborative Innovation Center for Eco-textiles of Shandong Province and the Ministry of Education, Qingdao, 266071, China; Corresponding author. Institute of Neuroregeneration and Neurorehabilitation, Qingdao Medical College, Qingdao University, Qingdao, 266071, China.In tissue repair, cell behaviors can be modulated by sensing and responding to the topographical and biochemical cues provided by the surrounding microenvironment. Herein, we designed and fabricated a class of uniaxially aligned nanofiber yarns welded with electrosprayed microparticles to guide the directional growth of axons and the migration of neural stem cells. By tuning the depositing density of microparticles on the nanofiber yarns, the surface roughness was varied, which facilitated the directional axon outgrowth of PC12 and SH-SY5Y cells and the alignment of Schwann cells. The microparticles were further modified by loading nerve growth factors in the core and then deposited on the nanofiber yarns. Owing to the synthetic effects provided by the physical and biological signals, the migration of neural stem cells was significantly accelerated. Taken together, the uniaxially aligned nanofiber yarns welded by functionalized microparticles show promising use in manipulating axonal growth and the migration of neural stem cells, which will provide a novel approach to clinical repair of nerve injuries.http://www.sciencedirect.com/science/article/pii/S2590049823000036Electrospun nanofiber yarnsMicroparticlesAxonal growthCell migrationNeural stem cells |
| spellingShingle | Xiaopei Zhang Mingxia Guo Qingxia Guo Na Liu Yuanfei Wang Tong Wu Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticles Materials Today Advances Electrospun nanofiber yarns Microparticles Axonal growth Cell migration Neural stem cells |
| title | Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticles |
| title_full | Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticles |
| title_fullStr | Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticles |
| title_full_unstemmed | Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticles |
| title_short | Modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with NGF-loaded microparticles |
| title_sort | modulating axonal growth and neural stem cell migration with the use of uniaxially aligned nanofiber yarns welded with ngf loaded microparticles |
| topic | Electrospun nanofiber yarns Microparticles Axonal growth Cell migration Neural stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2590049823000036 |
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