In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model
Poultry red mites (<i>Dermanyssus gallinae</i>, PRMs), tropical fowl mites (<i>Ornithonyssus bursa</i>, TFMs), and northern fowl mites (<i>O. sylviarum,</i> NFMs) are blood-feeding pests that debilitate poultry worldwide. Glutathione S-transferase (GST) plays an i...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/12/2/148 |
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| author | Shwe Yee Win Hikari Seo Fumiya Horio Sotaro Fujisawa Jumpei Sato Yoshinosuke Motai Takumi Sato Eiji Oishi Akira Taneno Lat Lat Htun Saw Bawm Tomohiro Okagawa Naoya Maekawa Satoru Konnai Kazuhiko Ohashi Shiro Murata |
| author_facet | Shwe Yee Win Hikari Seo Fumiya Horio Sotaro Fujisawa Jumpei Sato Yoshinosuke Motai Takumi Sato Eiji Oishi Akira Taneno Lat Lat Htun Saw Bawm Tomohiro Okagawa Naoya Maekawa Satoru Konnai Kazuhiko Ohashi Shiro Murata |
| author_sort | Shwe Yee Win |
| collection | DOAJ |
| description | Poultry red mites (<i>Dermanyssus gallinae</i>, PRMs), tropical fowl mites (<i>Ornithonyssus bursa</i>, TFMs), and northern fowl mites (<i>O. sylviarum,</i> NFMs) are blood-feeding pests that debilitate poultry worldwide. Glutathione S-transferase (GST) plays an important role in the detoxification and drug metabolism of mites. However, research on avian mite GSTs as vaccine antigens is still lacking. Therefore, we aimed to evaluate the potential of avian mite GSTs for vaccine development. We identified GST genes from TFMs and NFMs. We prepared recombinant GST (rGST) from TFMs, NFMs, and PRMs, and assessed their protein functions. Moreover, we evaluated the cross-reactivity and acaricidal effect of immune plasma against each rGST on TFMs, NFMs, and PRMs. The deduced amino acid sequences of GSTs from TFMs and NFMs were 80% similar to those of the PRMs. The rGSTs exhibited catalytic activity in conjugating glutathione to the 1-chloro-2,4-dinitrobenzene substrate. Immune plasma against each rGST showed cross-reactivity with rGST from different mite species. Moreover, the survival rate of PRMs fed with immune plasma against the rGST of TFMs and NFMs was significantly lower than that of the control plasma. These results demonstrate the potential application of GST as an antigen for the development of a broad-spectrum vaccine against avian mites. |
| first_indexed | 2024-03-07T22:11:25Z |
| format | Article |
| id | doaj.art-cb77113d2ef0450a9f50d657494ebbd9 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-07T22:11:25Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-cb77113d2ef0450a9f50d657494ebbd92024-02-23T15:36:59ZengMDPI AGVaccines2076-393X2024-01-0112214810.3390/vaccines12020148In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding ModelShwe Yee Win0Hikari Seo1Fumiya Horio2Sotaro Fujisawa3Jumpei Sato4Yoshinosuke Motai5Takumi Sato6Eiji Oishi7Akira Taneno8Lat Lat Htun9Saw Bawm10Tomohiro Okagawa11Naoya Maekawa12Satoru Konnai13Kazuhiko Ohashi14Shiro Murata15Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanVaxxinova Japan K.K., 1-24-8 Hamamatsucho, Minato-ku, Tokyo 105-0013, JapanVaxxinova Japan K.K., 1-24-8 Hamamatsucho, Minato-ku, Tokyo 105-0013, JapanVaxxinova Japan K.K., 1-24-8 Hamamatsucho, Minato-ku, Tokyo 105-0013, JapanDepartment of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, MyanmarDepartment of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, MyanmarDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanLaboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, JapanPoultry red mites (<i>Dermanyssus gallinae</i>, PRMs), tropical fowl mites (<i>Ornithonyssus bursa</i>, TFMs), and northern fowl mites (<i>O. sylviarum,</i> NFMs) are blood-feeding pests that debilitate poultry worldwide. Glutathione S-transferase (GST) plays an important role in the detoxification and drug metabolism of mites. However, research on avian mite GSTs as vaccine antigens is still lacking. Therefore, we aimed to evaluate the potential of avian mite GSTs for vaccine development. We identified GST genes from TFMs and NFMs. We prepared recombinant GST (rGST) from TFMs, NFMs, and PRMs, and assessed their protein functions. Moreover, we evaluated the cross-reactivity and acaricidal effect of immune plasma against each rGST on TFMs, NFMs, and PRMs. The deduced amino acid sequences of GSTs from TFMs and NFMs were 80% similar to those of the PRMs. The rGSTs exhibited catalytic activity in conjugating glutathione to the 1-chloro-2,4-dinitrobenzene substrate. Immune plasma against each rGST showed cross-reactivity with rGST from different mite species. Moreover, the survival rate of PRMs fed with immune plasma against the rGST of TFMs and NFMs was significantly lower than that of the control plasma. These results demonstrate the potential application of GST as an antigen for the development of a broad-spectrum vaccine against avian mites.https://www.mdpi.com/2076-393X/12/2/148poultry red mitetropical fowl mitenorthern fowl miteglutathione S-transferasevaccine |
| spellingShingle | Shwe Yee Win Hikari Seo Fumiya Horio Sotaro Fujisawa Jumpei Sato Yoshinosuke Motai Takumi Sato Eiji Oishi Akira Taneno Lat Lat Htun Saw Bawm Tomohiro Okagawa Naoya Maekawa Satoru Konnai Kazuhiko Ohashi Shiro Murata In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model Vaccines poultry red mite tropical fowl mite northern fowl mite glutathione S-transferase vaccine |
| title | In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model |
| title_full | In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model |
| title_fullStr | In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model |
| title_full_unstemmed | In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model |
| title_short | In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model |
| title_sort | in vivo characterization of the anti glutathione s transferase antibody using an in vitro mite feeding model |
| topic | poultry red mite tropical fowl mite northern fowl mite glutathione S-transferase vaccine |
| url | https://www.mdpi.com/2076-393X/12/2/148 |
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