Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells
Intermediate filaments (IFs) maintain cell–cell adhesions and are involved in diverse cellular processes such as cytokinesis, cell migration and the maintenance of cell structure. In this study, we investigated the influence of prostaglandin F2 alpha (PGF2α) on cytokeratin and vimentin IFs, Rho-asso...
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MDPI AG
2021-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/4/1073 |
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| author | Sang-Hee Lee Seunghyung Lee |
| author_facet | Sang-Hee Lee Seunghyung Lee |
| author_sort | Sang-Hee Lee |
| collection | DOAJ |
| description | Intermediate filaments (IFs) maintain cell–cell adhesions and are involved in diverse cellular processes such as cytokinesis, cell migration and the maintenance of cell structure. In this study, we investigated the influence of prostaglandin F2 alpha (PGF2α) on cytokeratin and vimentin IFs, Rho-associated protein kinase (ROCK), and cell-cell adhesion in bovine luteal theca cells (LTCs). The luteal cells were isolated from bovine corpus luteum (CL), and the LTCs were treated with 0, 0.01, 0.1 and 1.0 mM PGF2α. Cytokeratin, vimentin and desmoplakin proteins were disrupted and the ROCK protein was significantly increased in PGF2α-treated LTCs. In addition, cell–cell adhesion was significantly (<i>p</i> < 0.05) decreased in the PGF2α-induced LTCs compared to control group (0 mM PGF2α). In conclusion, PGF2α affected the adhesion of cell to cell via disruption of desmoplakin, cytokeratin and vimentin, additionally increasing ROCK in bovine LTCs. These results may provide a better understanding of the mechanism of bovine CL regression. |
| first_indexed | 2024-03-10T12:27:23Z |
| format | Article |
| id | doaj.art-cb77a4fc15ff4f9a9b206f14fcf5960a |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:27:23Z |
| publishDate | 2021-04-01 |
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| spelling | doaj.art-cb77a4fc15ff4f9a9b206f14fcf5960a2023-11-21T14:55:27ZengMDPI AGAnimals2076-26152021-04-01114107310.3390/ani11041073Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca CellsSang-Hee Lee0Seunghyung Lee1School of Information and Communications Technology, University of Tasmania, Hobart, TAS 7001, AustraliaCollege of Animal Life Sciences, Kangwon National University, Chuncheon 24341, KoreaIntermediate filaments (IFs) maintain cell–cell adhesions and are involved in diverse cellular processes such as cytokinesis, cell migration and the maintenance of cell structure. In this study, we investigated the influence of prostaglandin F2 alpha (PGF2α) on cytokeratin and vimentin IFs, Rho-associated protein kinase (ROCK), and cell-cell adhesion in bovine luteal theca cells (LTCs). The luteal cells were isolated from bovine corpus luteum (CL), and the LTCs were treated with 0, 0.01, 0.1 and 1.0 mM PGF2α. Cytokeratin, vimentin and desmoplakin proteins were disrupted and the ROCK protein was significantly increased in PGF2α-treated LTCs. In addition, cell–cell adhesion was significantly (<i>p</i> < 0.05) decreased in the PGF2α-induced LTCs compared to control group (0 mM PGF2α). In conclusion, PGF2α affected the adhesion of cell to cell via disruption of desmoplakin, cytokeratin and vimentin, additionally increasing ROCK in bovine LTCs. These results may provide a better understanding of the mechanism of bovine CL regression.https://www.mdpi.com/2076-2615/11/4/1073ovarycorpus luteumluteolysiscell adhesionprostaglandin F2 alpha |
| spellingShingle | Sang-Hee Lee Seunghyung Lee Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells Animals ovary corpus luteum luteolysis cell adhesion prostaglandin F2 alpha |
| title | Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells |
| title_full | Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells |
| title_fullStr | Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells |
| title_full_unstemmed | Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells |
| title_short | Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells |
| title_sort | prostaglandin f2 alpha triggers the disruption of cell adhesion with cytokeratin and vimentin in bovine luteal theca cells |
| topic | ovary corpus luteum luteolysis cell adhesion prostaglandin F2 alpha |
| url | https://www.mdpi.com/2076-2615/11/4/1073 |
| work_keys_str_mv | AT sangheelee prostaglandinf2alphatriggersthedisruptionofcelladhesionwithcytokeratinandvimentininbovinelutealthecacells AT seunghyunglee prostaglandinf2alphatriggersthedisruptionofcelladhesionwithcytokeratinandvimentininbovinelutealthecacells |