Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study
(1) We wanted to assess the impact of Ang2 in RCT-induced changes in the environment of glioblastoma. (2) The effect of Ang2 overexpression in tumor cells was studied in the GL261 syngeneic immunocompetent model of GB in response to fractionated RCT. (3) We showed that RCT combined with Ang2 led to...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/12/3585 |
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| author | Charly Helaine Aurélie E. Ferré Marine M. Leblond Elodie A. Pérès Myriam Bernaudin Samuel Valable Edwige Petit |
| author_facet | Charly Helaine Aurélie E. Ferré Marine M. Leblond Elodie A. Pérès Myriam Bernaudin Samuel Valable Edwige Petit |
| author_sort | Charly Helaine |
| collection | DOAJ |
| description | (1) We wanted to assess the impact of Ang2 in RCT-induced changes in the environment of glioblastoma. (2) The effect of Ang2 overexpression in tumor cells was studied in the GL261 syngeneic immunocompetent model of GB in response to fractionated RCT. (3) We showed that RCT combined with Ang2 led to tumor clearance for the GL261-Ang2 group by acting on the tumor cells as well as on both vascular and immune compartments. (4) In vitro, Ang2 overexpression in GL261 cells exposed to RCT promoted senescence and induced robust genomic instability, leading to mitotic death. (5) Coculture experiments of GL261-Ang2 cells with RAW 264.7 cells resulted in a significant increase in macrophage migration, which was abrogated by the addition of soluble Tie2 receptor. (6) Together, these preclinical results showed that, combined with RCT, Ang2 acted in an autocrine manner by increasing GB cell senescence and in a paracrine manner by acting on the innate immune system while modulating the vascular tumor compartment. On this preclinical model, we found that an ectopic expression of Ang2 combined with RCT impedes tumor recurrence. |
| first_indexed | 2024-03-10T14:25:36Z |
| format | Article |
| id | doaj.art-cb7ad1806d584aaca72449849e4251d6 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T14:25:36Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-cb7ad1806d584aaca72449849e4251d62023-11-20T23:02:23ZengMDPI AGCancers2072-66942020-11-011212358510.3390/cancers12123585Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical StudyCharly Helaine0Aurélie E. Ferré1Marine M. Leblond2Elodie A. Pérès3Myriam Bernaudin4Samuel Valable5Edwige Petit6UNICAEN, CNRS, CEA, ISTCT/CERVOxy Group, GIP Cyceron, Normandie University, 14000 Caen, FranceUNICAEN, CNRS, CEA, ISTCT/CERVOxy Group, GIP Cyceron, Normandie University, 14000 Caen, FranceUNICAEN, CNRS, CEA, ISTCT/CERVOxy Group, GIP Cyceron, Normandie University, 14000 Caen, FranceUNICAEN, CNRS, CEA, ISTCT/CERVOxy Group, GIP Cyceron, Normandie University, 14000 Caen, FranceUNICAEN, CNRS, CEA, ISTCT/CERVOxy Group, GIP Cyceron, Normandie University, 14000 Caen, FranceUNICAEN, CNRS, CEA, ISTCT/CERVOxy Group, GIP Cyceron, Normandie University, 14000 Caen, FranceUNICAEN, CNRS, CEA, ISTCT/CERVOxy Group, GIP Cyceron, Normandie University, 14000 Caen, France(1) We wanted to assess the impact of Ang2 in RCT-induced changes in the environment of glioblastoma. (2) The effect of Ang2 overexpression in tumor cells was studied in the GL261 syngeneic immunocompetent model of GB in response to fractionated RCT. (3) We showed that RCT combined with Ang2 led to tumor clearance for the GL261-Ang2 group by acting on the tumor cells as well as on both vascular and immune compartments. (4) In vitro, Ang2 overexpression in GL261 cells exposed to RCT promoted senescence and induced robust genomic instability, leading to mitotic death. (5) Coculture experiments of GL261-Ang2 cells with RAW 264.7 cells resulted in a significant increase in macrophage migration, which was abrogated by the addition of soluble Tie2 receptor. (6) Together, these preclinical results showed that, combined with RCT, Ang2 acted in an autocrine manner by increasing GB cell senescence and in a paracrine manner by acting on the innate immune system while modulating the vascular tumor compartment. On this preclinical model, we found that an ectopic expression of Ang2 combined with RCT impedes tumor recurrence.https://www.mdpi.com/2072-6694/12/12/3585glioblastomaangiopoietin-2vascularizationinflammationmicroenvironmentradiotherapy |
| spellingShingle | Charly Helaine Aurélie E. Ferré Marine M. Leblond Elodie A. Pérès Myriam Bernaudin Samuel Valable Edwige Petit Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study Cancers glioblastoma angiopoietin-2 vascularization inflammation microenvironment radiotherapy |
| title | Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study |
| title_full | Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study |
| title_fullStr | Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study |
| title_full_unstemmed | Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study |
| title_short | Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study |
| title_sort | angiopoietin 2 combined with radiochemotherapy impedes glioblastoma recurrence by acting in an autocrine and paracrine manner a preclinical study |
| topic | glioblastoma angiopoietin-2 vascularization inflammation microenvironment radiotherapy |
| url | https://www.mdpi.com/2072-6694/12/12/3585 |
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