Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach
Abstract Background Diagnostic accuracy of endomyocardial biopsy could improve if clinically safe magnetic resonance (MR)-compatible bioptomes were available. We explored two novel MR-compatible cardiac bioptomes for performance, safety, and clinical viability, employing in vivo minipig trials and p...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-12-01
|
| Series: | European Radiology Experimental |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41747-023-00391-4 |
| _version_ | 1797398244734533632 |
|---|---|
| author | Angelika Svetlove Christian O. Ritter Christian Dullin Michael Schmid Senta Schauer Johannes Uihlein Martin Uecker Matthias Mietsch Christine Stadelmann Joachim Lotz Christina Unterberg-Buchwald |
| author_facet | Angelika Svetlove Christian O. Ritter Christian Dullin Michael Schmid Senta Schauer Johannes Uihlein Martin Uecker Matthias Mietsch Christine Stadelmann Joachim Lotz Christina Unterberg-Buchwald |
| author_sort | Angelika Svetlove |
| collection | DOAJ |
| description | Abstract Background Diagnostic accuracy of endomyocardial biopsy could improve if clinically safe magnetic resonance (MR)-compatible bioptomes were available. We explored two novel MR-compatible cardiac bioptomes for performance, safety, and clinical viability, employing in vivo minipig trials and phase-contrast synchrotron radiation computed microtomography (SRµCT). Methods Analysis of ex vivo obtained pig endomyocardial biopsies was performed using phase-contrast SRµCT and conventional two-dimensional histology. The technical performance was evaluated by measuring volume, inner and outer integrities, compression, and histological diagnostic value in 3 sets (6 per set) of biopsies for each experimental bioptome. The bioptomes were tested in vivo in 3 healthy minipigs per bioptome. The clinical feasibility was evaluated by procedural and cutting success as well as histological diagnostic value. Results The bioptome with the ‘grind-grind’ design achieved similar values to control in compression (p = 0.822), inner (p = 0.628), and outer (p = 0.507), integrities ex vivo. It showed a better performance in the in vivo real-time MRI setting demonstrating a higher cutting success (91.7%) than the ‘grind-anvil’ (86.2%) design. In both ex vivo and in vivo evaluations, the ‘grind-grind’ design displayed sufficient diagnostic value (83% and 95%). The ‘grind-anvil’ design showed adequate diagnostic value both ex vivo and in vivo (78% and 87.5%) but was not comparable to control according to the three-dimensional (3D) analysis. Conclusion A novel MR-compatible bioptome was identified as plausible in a clinical setting. Additionally, SRµCT and subsequent 3D structural analysis could be valuable in the label-free investigation of myocardial tissue at a micrometer level. Relevance statement Implementation of MR-guided biopsy can improve animal studies on structural myocardial changes at any point in an experimental setup. With further improvements in guiding catheters, MR-guided biopsy, using the new bioptome, has a potential to increase quality and diagnostic accuracy in patients both with structural and inflammatory cardiomyopathies. Key points • Novel MR-compatible bioptomes show promise for a clinical application. • SRµCT enabled detailed analysis of endomyocardial biopsies. • The bioptomes showed adequate in vivo performance without major complications. Graphical Abstract |
| first_indexed | 2024-03-09T01:21:49Z |
| format | Article |
| id | doaj.art-cb7d4f9ed9fc44d0b956a8f8298cf33d |
| institution | Directory Open Access Journal |
| issn | 2509-9280 |
| language | English |
| last_indexed | 2024-03-09T01:21:49Z |
| publishDate | 2023-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | European Radiology Experimental |
| spelling | doaj.art-cb7d4f9ed9fc44d0b956a8f8298cf33d2023-12-10T12:06:31ZengSpringerOpenEuropean Radiology Experimental2509-92802023-12-017111110.1186/s41747-023-00391-4Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approachAngelika Svetlove0Christian O. Ritter1Christian Dullin2Michael Schmid3Senta Schauer4Johannes Uihlein5Martin Uecker6Matthias Mietsch7Christine Stadelmann8Joachim Lotz9Christina Unterberg-Buchwald10Translational Molecular Imaging, Max Planck Institute for Multidisciplinary SciencesInstitute for Diagnostic and Interventional Radiology, University Medical CentreTranslational Molecular Imaging, Max Planck Institute for Multidisciplinary SciencesEPflex Feinwerktechnik GmbHEPflex Feinwerktechnik GmbHEPflex Feinwerktechnik GmbHInstitute for Diagnostic and Interventional Radiology, University Medical CentreDZHK (German Centre for Cardiovascular Research), Partner Site GöttingenDepartment of Neuropathology, University Medical CentreInstitute for Diagnostic and Interventional Radiology, University Medical CentreInstitute for Diagnostic and Interventional Radiology, University Medical CentreAbstract Background Diagnostic accuracy of endomyocardial biopsy could improve if clinically safe magnetic resonance (MR)-compatible bioptomes were available. We explored two novel MR-compatible cardiac bioptomes for performance, safety, and clinical viability, employing in vivo minipig trials and phase-contrast synchrotron radiation computed microtomography (SRµCT). Methods Analysis of ex vivo obtained pig endomyocardial biopsies was performed using phase-contrast SRµCT and conventional two-dimensional histology. The technical performance was evaluated by measuring volume, inner and outer integrities, compression, and histological diagnostic value in 3 sets (6 per set) of biopsies for each experimental bioptome. The bioptomes were tested in vivo in 3 healthy minipigs per bioptome. The clinical feasibility was evaluated by procedural and cutting success as well as histological diagnostic value. Results The bioptome with the ‘grind-grind’ design achieved similar values to control in compression (p = 0.822), inner (p = 0.628), and outer (p = 0.507), integrities ex vivo. It showed a better performance in the in vivo real-time MRI setting demonstrating a higher cutting success (91.7%) than the ‘grind-anvil’ (86.2%) design. In both ex vivo and in vivo evaluations, the ‘grind-grind’ design displayed sufficient diagnostic value (83% and 95%). The ‘grind-anvil’ design showed adequate diagnostic value both ex vivo and in vivo (78% and 87.5%) but was not comparable to control according to the three-dimensional (3D) analysis. Conclusion A novel MR-compatible bioptome was identified as plausible in a clinical setting. Additionally, SRµCT and subsequent 3D structural analysis could be valuable in the label-free investigation of myocardial tissue at a micrometer level. Relevance statement Implementation of MR-guided biopsy can improve animal studies on structural myocardial changes at any point in an experimental setup. With further improvements in guiding catheters, MR-guided biopsy, using the new bioptome, has a potential to increase quality and diagnostic accuracy in patients both with structural and inflammatory cardiomyopathies. Key points • Novel MR-compatible bioptomes show promise for a clinical application. • SRµCT enabled detailed analysis of endomyocardial biopsies. • The bioptomes showed adequate in vivo performance without major complications. Graphical Abstracthttps://doi.org/10.1186/s41747-023-00391-4BiopsyMagnetic resonance imaging (interventional)SynchrotronsX-ray microtomography |
| spellingShingle | Angelika Svetlove Christian O. Ritter Christian Dullin Michael Schmid Senta Schauer Johannes Uihlein Martin Uecker Matthias Mietsch Christine Stadelmann Joachim Lotz Christina Unterberg-Buchwald Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach European Radiology Experimental Biopsy Magnetic resonance imaging (interventional) Synchrotrons X-ray microtomography |
| title | Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach |
| title_full | Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach |
| title_fullStr | Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach |
| title_full_unstemmed | Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach |
| title_short | Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach |
| title_sort | evaluation of mr safe bioptomes for mr guided endomyocardial biopsy in minipigs a potential radiation free clinical approach |
| topic | Biopsy Magnetic resonance imaging (interventional) Synchrotrons X-ray microtomography |
| url | https://doi.org/10.1186/s41747-023-00391-4 |
| work_keys_str_mv | AT angelikasvetlove evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT christianoritter evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT christiandullin evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT michaelschmid evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT sentaschauer evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT johannesuihlein evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT martinuecker evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT matthiasmietsch evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT christinestadelmann evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT joachimlotz evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach AT christinaunterbergbuchwald evaluationofmrsafebioptomesformrguidedendomyocardialbiopsyinminipigsapotentialradiationfreeclinicalapproach |