Detection of protein catalytic residues at high precision using local network properties
<p>Abstract</p> <p>Background</p> <p>Identifying the active site of an enzyme is a crucial step in functional studies. While protein sequences and structures can be experimentally characterized, determining which residues build up an active site is not a straightforward...
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| Format: | Article |
| Language: | English |
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BMC
2008-12-01
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| Series: | BMC Bioinformatics |
| Online Access: | http://www.biomedcentral.com/1471-2105/9/517 |
| _version_ | 1811250199665836032 |
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| author | Lappe Michael Filippis Ioannis Slama Patrick |
| author_facet | Lappe Michael Filippis Ioannis Slama Patrick |
| author_sort | Lappe Michael |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Identifying the active site of an enzyme is a crucial step in functional studies. While protein sequences and structures can be experimentally characterized, determining which residues build up an active site is not a straightforward process. In the present study a new method for the detection of protein active sites is introduced. This method uses local network descriptors derived from protein three-dimensional structures to determine whether a residue is part of an active site. It thus does not involve any sequence alignment or structure similarity to other proteins. A scoring function is elaborated over a set of more than 220 proteins having different structures and functions, in order to detect protein catalytic sites with a high precision, <it>i.e</it>. with a minimal rate of false positives.</p> <p>Results</p> <p>The scoring function was based on the counts of first-neighbours on side-chain contacts, third-neighbours and residue type. Precision of the detection using this function was 28.1%, which represents a more than three-fold increase compared to combining closeness centrality with residue surface accessibility, a function which was proposed in recent years. The performance of the scoring function was also analysed into detail over a smaller set of eight proteins. For the detection of 'functional' residues, which were involved either directly in catalytic activity or in the binding of substrates, precision reached a value of 72.7% on this second set. These results suggested that our scoring function was effective at detecting not only catalytic residues, but also any residue that is part of the functional site of a protein.</p> <p>Conclusion</p> <p>As having been validated on the majority of known structural families, this method should prove useful for the detection of active sites in any protein with unknown function, and for direct application to the design of site-directed mutagenesis experiments.</p> |
| first_indexed | 2024-04-12T16:00:48Z |
| format | Article |
| id | doaj.art-cb888824d0e9463ebce062ccfa25c922 |
| institution | Directory Open Access Journal |
| issn | 1471-2105 |
| language | English |
| last_indexed | 2024-04-12T16:00:48Z |
| publishDate | 2008-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Bioinformatics |
| spelling | doaj.art-cb888824d0e9463ebce062ccfa25c9222022-12-22T03:26:13ZengBMCBMC Bioinformatics1471-21052008-12-019151710.1186/1471-2105-9-517Detection of protein catalytic residues at high precision using local network propertiesLappe MichaelFilippis IoannisSlama Patrick<p>Abstract</p> <p>Background</p> <p>Identifying the active site of an enzyme is a crucial step in functional studies. While protein sequences and structures can be experimentally characterized, determining which residues build up an active site is not a straightforward process. In the present study a new method for the detection of protein active sites is introduced. This method uses local network descriptors derived from protein three-dimensional structures to determine whether a residue is part of an active site. It thus does not involve any sequence alignment or structure similarity to other proteins. A scoring function is elaborated over a set of more than 220 proteins having different structures and functions, in order to detect protein catalytic sites with a high precision, <it>i.e</it>. with a minimal rate of false positives.</p> <p>Results</p> <p>The scoring function was based on the counts of first-neighbours on side-chain contacts, third-neighbours and residue type. Precision of the detection using this function was 28.1%, which represents a more than three-fold increase compared to combining closeness centrality with residue surface accessibility, a function which was proposed in recent years. The performance of the scoring function was also analysed into detail over a smaller set of eight proteins. For the detection of 'functional' residues, which were involved either directly in catalytic activity or in the binding of substrates, precision reached a value of 72.7% on this second set. These results suggested that our scoring function was effective at detecting not only catalytic residues, but also any residue that is part of the functional site of a protein.</p> <p>Conclusion</p> <p>As having been validated on the majority of known structural families, this method should prove useful for the detection of active sites in any protein with unknown function, and for direct application to the design of site-directed mutagenesis experiments.</p>http://www.biomedcentral.com/1471-2105/9/517 |
| spellingShingle | Lappe Michael Filippis Ioannis Slama Patrick Detection of protein catalytic residues at high precision using local network properties BMC Bioinformatics |
| title | Detection of protein catalytic residues at high precision using local network properties |
| title_full | Detection of protein catalytic residues at high precision using local network properties |
| title_fullStr | Detection of protein catalytic residues at high precision using local network properties |
| title_full_unstemmed | Detection of protein catalytic residues at high precision using local network properties |
| title_short | Detection of protein catalytic residues at high precision using local network properties |
| title_sort | detection of protein catalytic residues at high precision using local network properties |
| url | http://www.biomedcentral.com/1471-2105/9/517 |
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