Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in Vitro
In this paper, Cinnamomum cassia polysaccharide (CCP) was extracted and purified, and its physicochemical properties, antioxidant and hypoglycemic activities were investigated. The polysaccharide yield was optimized by the response surface test with three conditions: Temperature, time and liquid-sol...
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The editorial department of Science and Technology of Food Industry
2024-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023080088 |
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| author | Huihui ZHANG Can LI Huiping LIU Xiaoxiao MA Xin ZHANG Bing WANG Ying LIU |
| author_facet | Huihui ZHANG Can LI Huiping LIU Xiaoxiao MA Xin ZHANG Bing WANG Ying LIU |
| author_sort | Huihui ZHANG |
| collection | DOAJ |
| description | In this paper, Cinnamomum cassia polysaccharide (CCP) was extracted and purified, and its physicochemical properties, antioxidant and hypoglycemic activities were investigated. The polysaccharide yield was optimized by the response surface test with three conditions: Temperature, time and liquid-solid ratio. Then the physicochemical properties of purified CCP were investigated by high performance liquid chromatography, thermogravimetric analysis and other methods, meanwhile, its antioxidant and hypoglycemic activities were tested in vitro. Results indicated that the optimum extraction conditions as follow: Extraction temperature 90 ℃, extraction time 2.5 h, liquid-solid ratio 20:1 (mL/g), extraction twice, under these conditions, the average yield was 3.22%. The relative molecular weight of CCP was 1.95×106 Da, the total sugar content was 90.11%±1.24%, and it was stable within 230 ℃. Both Fourier-transform infrared and nuclear magnetic hydrogen spectra showed that CCP had α, β-glycosidic bonds and other structures, in addition, Congo red staining showed that it might not have triple helix structure. Activity assays showed that CCP had good scavenging effect on DPPH·, ABTS+∙, ·OH, with IC50 values of 0.191, 2.835 and 3.221 mg/mL, respectively, and the total reducing power value of the polysaccharide at 6 mg/mL was 1.18. In addition, CCP also had inhibitory activity against α-amylase and α-glucosidase, with IC50 values of 0.189 and 0.340 mg/mL. In this study, a high molecular weight Cinnamomum cassia polysaccharide was extracted and purified, which had good antioxidant and hypoglycemic activities, which may provide a basis for the functional development and utilization of Cinnamomum cassia. |
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| language | zho |
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| publishDate | 2024-04-01 |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-cb8a29b5fdef4c58b9de6b861594262e2024-03-22T06:45:48ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-04-01457152410.13386/j.issn1002-0306.20230800882023080088-7Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in VitroHuihui ZHANG0Can LI1Huiping LIU2Xiaoxiao MA3Xin ZHANG4Bing WANG5Ying LIU6College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaIn this paper, Cinnamomum cassia polysaccharide (CCP) was extracted and purified, and its physicochemical properties, antioxidant and hypoglycemic activities were investigated. The polysaccharide yield was optimized by the response surface test with three conditions: Temperature, time and liquid-solid ratio. Then the physicochemical properties of purified CCP were investigated by high performance liquid chromatography, thermogravimetric analysis and other methods, meanwhile, its antioxidant and hypoglycemic activities were tested in vitro. Results indicated that the optimum extraction conditions as follow: Extraction temperature 90 ℃, extraction time 2.5 h, liquid-solid ratio 20:1 (mL/g), extraction twice, under these conditions, the average yield was 3.22%. The relative molecular weight of CCP was 1.95×106 Da, the total sugar content was 90.11%±1.24%, and it was stable within 230 ℃. Both Fourier-transform infrared and nuclear magnetic hydrogen spectra showed that CCP had α, β-glycosidic bonds and other structures, in addition, Congo red staining showed that it might not have triple helix structure. Activity assays showed that CCP had good scavenging effect on DPPH·, ABTS+∙, ·OH, with IC50 values of 0.191, 2.835 and 3.221 mg/mL, respectively, and the total reducing power value of the polysaccharide at 6 mg/mL was 1.18. In addition, CCP also had inhibitory activity against α-amylase and α-glucosidase, with IC50 values of 0.189 and 0.340 mg/mL. In this study, a high molecular weight Cinnamomum cassia polysaccharide was extracted and purified, which had good antioxidant and hypoglycemic activities, which may provide a basis for the functional development and utilization of Cinnamomum cassia.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023080088cinnamomum cassia polysaccharidesoptimization of extraction processphysical and chemical analysisantioxidant activityhypoglycemic activity |
| spellingShingle | Huihui ZHANG Can LI Huiping LIU Xiaoxiao MA Xin ZHANG Bing WANG Ying LIU Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in Vitro Shipin gongye ke-ji cinnamomum cassia polysaccharides optimization of extraction process physical and chemical analysis antioxidant activity hypoglycemic activity |
| title | Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in Vitro |
| title_full | Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in Vitro |
| title_fullStr | Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in Vitro |
| title_full_unstemmed | Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in Vitro |
| title_short | Extraction and Purification of Cinnamomum cassia Polysaccharides and Its Antioxidant and Hypoglycemic Activities in Vitro |
| title_sort | extraction and purification of cinnamomum cassia polysaccharides and its antioxidant and hypoglycemic activities in vitro |
| topic | cinnamomum cassia polysaccharides optimization of extraction process physical and chemical analysis antioxidant activity hypoglycemic activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023080088 |
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