Optimization of Culture Conditions for Secretory Production of 3-Hydroxybutyrate Oligomers Using Recombinant Escherichia coli

Optimization of Culture Conditions for Secretory Production of 3-Hydroxybutyrate Oligomers Using Recombinant Escherichia coli

Poly(3-hydroxybutyrate) [P(3HB)] is the most representative polyhydroxyalkanoate (PHA), which is a storage polyester for prokaryotic cells. P(3HB)-producing recombinant Escherichia coli secretes diethylene glycol (DEG)-terminated 3HB oligomers (3HBO-DEG) through a PHA synthase-mediated chain transfe...

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Bibliographic Details
Main Authors:	Tetsuo Sakurai, Shoji Mizuno, Yuki Miyahara, Ayaka Hiroe, Seiichi Taguchi, Takeharu Tsuge
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022-02-01
Series:	Frontiers in Bioengineering and Biotechnology
Subjects:	class IV PHA synthase oligomer secretion fed-batch culture alcoholysis chain transfer reaction
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2022.829134/full

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