Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism
Abstract Two-photon absorption (TPA) fluorescence imaging holds great promise in diagnostics and biomedicine owing to its unparalleled spatiotemporal resolution. However, the adaptability and applicability of currently available TPA probes, which act as a critical element for determining the imaging...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40897-4 |
| _version_ | 1797558612539736064 |
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| author | Shukun Li Rui Chang Luyang Zhao Ruirui Xing Jan C. M. van Hest Xuehai Yan |
| author_facet | Shukun Li Rui Chang Luyang Zhao Ruirui Xing Jan C. M. van Hest Xuehai Yan |
| author_sort | Shukun Li |
| collection | DOAJ |
| description | Abstract Two-photon absorption (TPA) fluorescence imaging holds great promise in diagnostics and biomedicine owing to its unparalleled spatiotemporal resolution. However, the adaptability and applicability of currently available TPA probes, which act as a critical element for determining the imaging contrast effect, is severely challenged by limited photo-luminescence in vivo. This is particularly a result of uncontrollable aggregation that causes fluorescence quenching, and inevitable photo-oxidation in harsh physiological milieu, which normally leads to bleaching of the dye. Herein, we describe the remarkably enhanced TPA fluorescence imaging capacity of self-assembling near-infrared (NIR) cyanine dye-based nanoprobes (NPs), which can be explained by a photo-oxidation enhanced emission mechanism. Singlet oxygen generated during photo-oxidation enables chromophore dimerization to form TPA intermediates responsible for enhanced TPA fluorescence emission. The resulting NPs possess uniform size distribution, excellent stability, more favorable TPA cross-section and anti-bleaching ability than a popular TPA probe rhodamine B (RhB). These properties of cyanine dye-based TPA NPs promote their applications in visualizing blood circulation and tumoral accumulation in real-time, even to cellular imaging in vivo. The photo-oxidation enhanced emission mechanism observed in these near-infrared cyanine dye-based nanoaggregates opens an avenue for design and development of more advanced TPA fluorescence probes. |
| first_indexed | 2024-03-10T17:33:56Z |
| format | Article |
| id | doaj.art-cb8d766024da4d4f98dc8753eba29d6f |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:33:56Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-cb8d766024da4d4f98dc8753eba29d6f2023-11-20T09:55:49ZengNature PortfolioNature Communications2041-17232023-08-0114111310.1038/s41467-023-40897-4Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanismShukun Li0Rui Chang1Luyang Zhao2Ruirui Xing3Jan C. M. van Hest4Xuehai Yan5State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringState Key Laboratory of Biochemical Engineering, Institute of Process EngineeringState Key Laboratory of Biochemical Engineering, Institute of Process EngineeringState Key Laboratory of Biochemical Engineering, Institute of Process EngineeringBio-Organic Chemistry, Institute for Complex Molecular Systems, Eindhoven University of TechnologyState Key Laboratory of Biochemical Engineering, Institute of Process EngineeringAbstract Two-photon absorption (TPA) fluorescence imaging holds great promise in diagnostics and biomedicine owing to its unparalleled spatiotemporal resolution. However, the adaptability and applicability of currently available TPA probes, which act as a critical element for determining the imaging contrast effect, is severely challenged by limited photo-luminescence in vivo. This is particularly a result of uncontrollable aggregation that causes fluorescence quenching, and inevitable photo-oxidation in harsh physiological milieu, which normally leads to bleaching of the dye. Herein, we describe the remarkably enhanced TPA fluorescence imaging capacity of self-assembling near-infrared (NIR) cyanine dye-based nanoprobes (NPs), which can be explained by a photo-oxidation enhanced emission mechanism. Singlet oxygen generated during photo-oxidation enables chromophore dimerization to form TPA intermediates responsible for enhanced TPA fluorescence emission. The resulting NPs possess uniform size distribution, excellent stability, more favorable TPA cross-section and anti-bleaching ability than a popular TPA probe rhodamine B (RhB). These properties of cyanine dye-based TPA NPs promote their applications in visualizing blood circulation and tumoral accumulation in real-time, even to cellular imaging in vivo. The photo-oxidation enhanced emission mechanism observed in these near-infrared cyanine dye-based nanoaggregates opens an avenue for design and development of more advanced TPA fluorescence probes.https://doi.org/10.1038/s41467-023-40897-4 |
| spellingShingle | Shukun Li Rui Chang Luyang Zhao Ruirui Xing Jan C. M. van Hest Xuehai Yan Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism Nature Communications |
| title | Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism |
| title_full | Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism |
| title_fullStr | Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism |
| title_full_unstemmed | Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism |
| title_short | Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism |
| title_sort | two photon nanoprobes based on bioorganic nanoarchitectonics with a photo oxidation enhanced emission mechanism |
| url | https://doi.org/10.1038/s41467-023-40897-4 |
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