Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass Propagation
Peroxiredoxins are a family of peroxide-degrading enzymes for challenging oxidative stress. They receive their reducing power from redox-controlling proteins called thioredoxins, and these, in turn, from thioredoxin reductase. The main cytosolic peroxiredoxin is Tsa1, a moonlighting protein that als...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1537 |
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| author | Víctor Garrigós Cecilia Picazo Emilia Matallana Agustín Aranda |
| author_facet | Víctor Garrigós Cecilia Picazo Emilia Matallana Agustín Aranda |
| author_sort | Víctor Garrigós |
| collection | DOAJ |
| description | Peroxiredoxins are a family of peroxide-degrading enzymes for challenging oxidative stress. They receive their reducing power from redox-controlling proteins called thioredoxins, and these, in turn, from thioredoxin reductase. The main cytosolic peroxiredoxin is Tsa1, a moonlighting protein that also acts as protein chaperone a redox switch controlling some metabolic events. Gene deletion of peroxiredoxins in wine yeasts indicate that <i>TSA1</i>, thioredoxins and thioredoxin reductase <i>TRR1</i> are required for normal growth in medium with glucose and sucrose as carbon sources. <i>TSA1</i> gene deletion also diminishes growth in molasses, both in flasks and bioreactors. The <i>TSA1</i> mutation brings about an expected change in redox parameters but, interestingly, it also triggers a variety of metabolic changes. It influences trehalose accumulation, lowering it in first molasses growth stages, but increasing it at the end of batch growth, when respiratory metabolism is set up. Glycogen accumulation at the entry of the stationary phase also increases in the <i>tsa1</i>Δ mutant. The mutation reduces fermentative capacity in grape juice, but the vinification profile does not significantly change. However, acetic acid and acetaldehyde production decrease when <i>TSA1</i> is absent. Hence, <i>TSA1</i> plays a role in the regulation of metabolic reactions leading to the production of such relevant enological molecules. |
| first_indexed | 2024-03-10T15:49:10Z |
| format | Article |
| id | doaj.art-cb8eee42d4cf4230b8d646fb0c4f112a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:49:10Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cb8eee42d4cf4230b8d646fb0c4f112a2023-11-20T16:11:41ZengMDPI AGMicroorganisms2076-26072020-10-01810153710.3390/microorganisms8101537Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass PropagationVíctor Garrigós0Cecilia Picazo1Emilia Matallana2Agustín Aranda3Institute for Integrative Systems Biology, I2SysBio, University of Valencia-CSIC, 7, 46980 Paterna, SpainInstitute for Integrative Systems Biology, I2SysBio, University of Valencia-CSIC, 7, 46980 Paterna, SpainInstitute for Integrative Systems Biology, I2SysBio, University of Valencia-CSIC, 7, 46980 Paterna, SpainInstitute for Integrative Systems Biology, I2SysBio, University of Valencia-CSIC, 7, 46980 Paterna, SpainPeroxiredoxins are a family of peroxide-degrading enzymes for challenging oxidative stress. They receive their reducing power from redox-controlling proteins called thioredoxins, and these, in turn, from thioredoxin reductase. The main cytosolic peroxiredoxin is Tsa1, a moonlighting protein that also acts as protein chaperone a redox switch controlling some metabolic events. Gene deletion of peroxiredoxins in wine yeasts indicate that <i>TSA1</i>, thioredoxins and thioredoxin reductase <i>TRR1</i> are required for normal growth in medium with glucose and sucrose as carbon sources. <i>TSA1</i> gene deletion also diminishes growth in molasses, both in flasks and bioreactors. The <i>TSA1</i> mutation brings about an expected change in redox parameters but, interestingly, it also triggers a variety of metabolic changes. It influences trehalose accumulation, lowering it in first molasses growth stages, but increasing it at the end of batch growth, when respiratory metabolism is set up. Glycogen accumulation at the entry of the stationary phase also increases in the <i>tsa1</i>Δ mutant. The mutation reduces fermentative capacity in grape juice, but the vinification profile does not significantly change. However, acetic acid and acetaldehyde production decrease when <i>TSA1</i> is absent. Hence, <i>TSA1</i> plays a role in the regulation of metabolic reactions leading to the production of such relevant enological molecules.https://www.mdpi.com/2076-2607/8/10/1537<i>Saccharomyces cerevisiae</i>winebiomassoxidative stressperoxiredoxinsTsa1 |
| spellingShingle | Víctor Garrigós Cecilia Picazo Emilia Matallana Agustín Aranda Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass Propagation Microorganisms <i>Saccharomyces cerevisiae</i> wine biomass oxidative stress peroxiredoxins Tsa1 |
| title | Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass Propagation |
| title_full | Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass Propagation |
| title_fullStr | Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass Propagation |
| title_full_unstemmed | Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass Propagation |
| title_short | Wine Yeast Peroxiredoxin <i>TSA1</i> Plays a Role in Growth, Stress Response and Trehalose Metabolism in Biomass Propagation |
| title_sort | wine yeast peroxiredoxin i tsa1 i plays a role in growth stress response and trehalose metabolism in biomass propagation |
| topic | <i>Saccharomyces cerevisiae</i> wine biomass oxidative stress peroxiredoxins Tsa1 |
| url | https://www.mdpi.com/2076-2607/8/10/1537 |
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