Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer
Ovarian cancer is the most common cause of gynaecological cancer‐associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpre...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-12-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12137 |
| _version_ | 1798014994953011200 |
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| author | Yaying Lin Rongrong Zhang Ping Zhang |
| author_facet | Yaying Lin Rongrong Zhang Ping Zhang |
| author_sort | Yaying Lin |
| collection | DOAJ |
| description | Ovarian cancer is the most common cause of gynaecological cancer‐associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpressed in ovarian cancer clinical tissues. Furthermore, the expression of EIF3D was correlated with the International Federation of Gynecology and Obstetrics stage and pathological differentiation stage. 3‐(4,5‐dimethylthylthiazol‐2‐yl)‐2 (MTT) and colony formation assays revealed that the lentivirus‐mediated knockdown of EIF3D suppresses cell proliferation in the ovarian tumour cell lines CAOV‐3 and SKOV‐3. Flow cytometry revealed that cells were arrested at the G2/M phase of the cell cycle and that cyclin‐dependent kinase 1 was also altered after EIF3D silencing. The results presented here demonstrate that EIF3D may play an important role in the occurrence and development of ovarian cancer. |
| first_indexed | 2024-04-11T15:26:06Z |
| format | Article |
| id | doaj.art-cb9006dea49043f886270d244aff66f3 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T15:26:06Z |
| publishDate | 2016-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-cb9006dea49043f886270d244aff66f32022-12-22T04:16:14ZengWileyFEBS Open Bio2211-54632016-12-016121201121010.1002/2211-5463.12137Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancerYaying Lin0Rongrong Zhang1Ping Zhang2Department of Gynecology Xinhua Hospital Shanghai Jiao Tong University School of Medicine ChinaDepartment of Gynecology Xinhua Hospital Shanghai Jiao Tong University School of Medicine ChinaDepartment of Gynecology Xinhua Hospital Shanghai Jiao Tong University School of Medicine ChinaOvarian cancer is the most common cause of gynaecological cancer‐associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpressed in ovarian cancer clinical tissues. Furthermore, the expression of EIF3D was correlated with the International Federation of Gynecology and Obstetrics stage and pathological differentiation stage. 3‐(4,5‐dimethylthylthiazol‐2‐yl)‐2 (MTT) and colony formation assays revealed that the lentivirus‐mediated knockdown of EIF3D suppresses cell proliferation in the ovarian tumour cell lines CAOV‐3 and SKOV‐3. Flow cytometry revealed that cells were arrested at the G2/M phase of the cell cycle and that cyclin‐dependent kinase 1 was also altered after EIF3D silencing. The results presented here demonstrate that EIF3D may play an important role in the occurrence and development of ovarian cancer.https://doi.org/10.1002/2211-5463.12137cell cycleEIF3Dovarian cancerproliferation |
| spellingShingle | Yaying Lin Rongrong Zhang Ping Zhang Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer FEBS Open Bio cell cycle EIF3D ovarian cancer proliferation |
| title | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
| title_full | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
| title_fullStr | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
| title_full_unstemmed | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
| title_short | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
| title_sort | eukaryotic translation initiation factor 3 subunit d overexpression is associated with the occurrence and development of ovarian cancer |
| topic | cell cycle EIF3D ovarian cancer proliferation |
| url | https://doi.org/10.1002/2211-5463.12137 |
| work_keys_str_mv | AT yayinglin eukaryotictranslationinitiationfactor3subunitdoverexpressionisassociatedwiththeoccurrenceanddevelopmentofovariancancer AT rongrongzhang eukaryotictranslationinitiationfactor3subunitdoverexpressionisassociatedwiththeoccurrenceanddevelopmentofovariancancer AT pingzhang eukaryotictranslationinitiationfactor3subunitdoverexpressionisassociatedwiththeoccurrenceanddevelopmentofovariancancer |