Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose
Abstract Background Polygonum Multiflorum Thunb(PMT) has multiple biological effects, such as anti-inflammatory, lipid-lowering, anti-aging and so on. Therefore, D-galactose-induced aging mice were used to study the effect of PMT on fatty acid metabolism and its underlying mechanism. Methods C57BL/6...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-019-1055-y |
| _version_ | 1819298176846266368 |
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| author | Jiangquan Yang Yuqi He Jiayi Zou Lin Xu Fang Fan Zhenglong Ge |
| author_facet | Jiangquan Yang Yuqi He Jiayi Zou Lin Xu Fang Fan Zhenglong Ge |
| author_sort | Jiangquan Yang |
| collection | DOAJ |
| description | Abstract Background Polygonum Multiflorum Thunb(PMT) has multiple biological effects, such as anti-inflammatory, lipid-lowering, anti-aging and so on. Therefore, D-galactose-induced aging mice were used to study the effect of PMT on fatty acid metabolism and its underlying mechanism. Methods C57BL/6 male mice were randomly divided into normal group, aging model group, PMT intragastrical administration group (high, Medium, low); model group and PMT intragastrical administration group Daily intraperitoneal injection D-galactose 800 mg·ml− 1·Kg− 1 to establish subacute aging model; PMT intragastrical administration group at the same time to intragastrical PMT extract (1 g·ml− 1·Kg− 1, 0.6 g·ml− 1·Kg− 1, 0.3 g·ml− 1·Kg− 1), normal group injection and intragastrical equivalent saline for 60 consecutive days. By detecting the oxidation index of liver to judge the efficacy of PMT, gas chromatography-mass spectrometry (GC-MS) analysis was used to quantitatively analyze the fatty acid content in liver. Results Finally, we found that PMT improved the enzyme activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in aging mice, and reduce the enzyme activity of malondialdehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The content of fatty acids such as C18:1, C18:2, C18:3 N3, C20:2 and C20:3 N3 decreased significantly in senescent mice (P < 0.05) as evidenced by GC-MS analysis, whereas, these fatty acids increased significantly after treatment of PMT (P < 0.05). Conclusion PMT improves the content of liver fatty acids in aging mice induced by D-galactose through, enhancing the activity of anti-oxidant enzymes. |
| first_indexed | 2024-12-24T05:25:45Z |
| format | Article |
| id | doaj.art-cb9495e8c2524b3da468d4b1f0baf747 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-24T05:25:45Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-cb9495e8c2524b3da468d4b1f0baf7472022-12-21T17:13:20ZengBMCLipids in Health and Disease1476-511X2019-06-011811810.1186/s12944-019-1055-yEffect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactoseJiangquan Yang0Yuqi He1Jiayi Zou2Lin Xu3Fang Fan4Zhenglong Ge5Department of Biochemistry and Molecular Biology, Zunyi Medical UniversityDepartment of Pharmaceutical Analysis, Zunyi Medical UniversityDepartment of Biochemistry and Molecular Biology, Zunyi Medical UniversityDepartment of Biochemistry and Molecular Biology, Zunyi Medical UniversityDepartment of Biochemistry and Molecular Biology, Zunyi Medical UniversityDepartment of Biochemistry and Molecular Biology, Zunyi Medical UniversityAbstract Background Polygonum Multiflorum Thunb(PMT) has multiple biological effects, such as anti-inflammatory, lipid-lowering, anti-aging and so on. Therefore, D-galactose-induced aging mice were used to study the effect of PMT on fatty acid metabolism and its underlying mechanism. Methods C57BL/6 male mice were randomly divided into normal group, aging model group, PMT intragastrical administration group (high, Medium, low); model group and PMT intragastrical administration group Daily intraperitoneal injection D-galactose 800 mg·ml− 1·Kg− 1 to establish subacute aging model; PMT intragastrical administration group at the same time to intragastrical PMT extract (1 g·ml− 1·Kg− 1, 0.6 g·ml− 1·Kg− 1, 0.3 g·ml− 1·Kg− 1), normal group injection and intragastrical equivalent saline for 60 consecutive days. By detecting the oxidation index of liver to judge the efficacy of PMT, gas chromatography-mass spectrometry (GC-MS) analysis was used to quantitatively analyze the fatty acid content in liver. Results Finally, we found that PMT improved the enzyme activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in aging mice, and reduce the enzyme activity of malondialdehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The content of fatty acids such as C18:1, C18:2, C18:3 N3, C20:2 and C20:3 N3 decreased significantly in senescent mice (P < 0.05) as evidenced by GC-MS analysis, whereas, these fatty acids increased significantly after treatment of PMT (P < 0.05). Conclusion PMT improves the content of liver fatty acids in aging mice induced by D-galactose through, enhancing the activity of anti-oxidant enzymes.http://link.springer.com/article/10.1186/s12944-019-1055-yPolygonum Multiflorum ThunbAgingFatty acidsGas chromatography mass spectrometryD-galactose |
| spellingShingle | Jiangquan Yang Yuqi He Jiayi Zou Lin Xu Fang Fan Zhenglong Ge Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose Lipids in Health and Disease Polygonum Multiflorum Thunb Aging Fatty acids Gas chromatography mass spectrometry D-galactose |
| title | Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose |
| title_full | Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose |
| title_fullStr | Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose |
| title_full_unstemmed | Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose |
| title_short | Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose |
| title_sort | effect of polygonum multiflorum thunb on liver fatty acid content in aging mice induced by d galactose |
| topic | Polygonum Multiflorum Thunb Aging Fatty acids Gas chromatography mass spectrometry D-galactose |
| url | http://link.springer.com/article/10.1186/s12944-019-1055-y |
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