Screening of cis-acting elements for maintaining pluripotency of human embryonic stem cells

Screening of cis-acting elements for maintaining pluripotency of human embryonic stem cells

Objective To establish an inducible CRISPR library screening method for human embryonic stem cells (hESCs) to screen the cis-acting elements involed in the maintainance the pluripotency of hESCs. Methods The inducible Cas9 expression elements were inserted into the hESC genome to construct an indu...

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Bibliographic Details
Main Author:	SUN Meng-yao, LIU Si-qi, ZHOU Fan-qi, MA Yan-ni, YU Jia
Format:	Article
Language:	zho
Published:	Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-05-01
Series:	Jichu yixue yu linchuang
Subjects:	human embryonic stem cell\|pluripotency\|cis-elements\|crispr library
Online Access:	http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210082.pdf

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