Preparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticles
Abstract This study aimed to construct a Ginsenoside Rb1-PLGA nano drug delivery system, optimize its preparation process, characterize and evaluate the resulting Ginsenoside Rb1-PLGA Nanoparticles (GRb1@PLGA@NPs). GRb1@PLGA@NPs were prepared using the emulsion solvent evaporation method. The optima...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45858-x |
| _version_ | 1797647290192625664 |
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| author | Lixin Du Huiling Lu Yifei Xiao Zhihua Guo Ya Li |
| author_facet | Lixin Du Huiling Lu Yifei Xiao Zhihua Guo Ya Li |
| author_sort | Lixin Du |
| collection | DOAJ |
| description | Abstract This study aimed to construct a Ginsenoside Rb1-PLGA nano drug delivery system, optimize its preparation process, characterize and evaluate the resulting Ginsenoside Rb1-PLGA Nanoparticles (GRb1@PLGA@NPs). GRb1@PLGA@NPs were prepared using the emulsion solvent evaporation method. The optimal preparation process was determined using Plackett–Burman design combined with Box-Behnken experiments. Physical characterization and in vitro release studies were conducted. LC–MS/MS technique was employed to investigate the pharmacokinetic characteristics of GRb1 and GRb1@PLGA@NPs in rat plasma. The optimal preparation process yielded GRb1@PLGA@NPs with a particle size of 120.63 nm, polydispersity index (PDI) of 0.172, zeta potential of − 22.67 mV, encapsulation efficiency of 75%, and drug loading of 11%. In vitro release demonstrated sustained drug release. Compared to GRb1, GRb1@PLGA@NPs exhibited a shortened time to peak concentration by approximately 0.72-fold. The area under the plasma concentration–time curve significantly increased to 4.58-fold of GRb1. GRb1@PLGA@NPs formulated using the optimal process exhibited uniform distribution and stable quality, its relative oral bioavailability was significantly improved compared to free GRb1. |
| first_indexed | 2024-03-11T15:15:09Z |
| format | Article |
| id | doaj.art-cb98ff13cebe4c8ea9ed32eed4852692 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T15:15:09Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cb98ff13cebe4c8ea9ed32eed48526922023-10-29T12:24:11ZengNature PortfolioScientific Reports2045-23222023-10-0113111410.1038/s41598-023-45858-xPreparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticlesLixin Du0Huiling Lu1Yifei Xiao2Zhihua Guo3Ya Li4College of Pharmacy, Hunan University of Chinese MedicineCollege of Pharmacy, Hunan University of Chinese MedicineCollege of Pharmacy, Hunan University of Chinese MedicineCollege of Chinese Medicine, Hunan University of Chinese MedicineCollege of Pharmacy, Hunan University of Chinese MedicineAbstract This study aimed to construct a Ginsenoside Rb1-PLGA nano drug delivery system, optimize its preparation process, characterize and evaluate the resulting Ginsenoside Rb1-PLGA Nanoparticles (GRb1@PLGA@NPs). GRb1@PLGA@NPs were prepared using the emulsion solvent evaporation method. The optimal preparation process was determined using Plackett–Burman design combined with Box-Behnken experiments. Physical characterization and in vitro release studies were conducted. LC–MS/MS technique was employed to investigate the pharmacokinetic characteristics of GRb1 and GRb1@PLGA@NPs in rat plasma. The optimal preparation process yielded GRb1@PLGA@NPs with a particle size of 120.63 nm, polydispersity index (PDI) of 0.172, zeta potential of − 22.67 mV, encapsulation efficiency of 75%, and drug loading of 11%. In vitro release demonstrated sustained drug release. Compared to GRb1, GRb1@PLGA@NPs exhibited a shortened time to peak concentration by approximately 0.72-fold. The area under the plasma concentration–time curve significantly increased to 4.58-fold of GRb1. GRb1@PLGA@NPs formulated using the optimal process exhibited uniform distribution and stable quality, its relative oral bioavailability was significantly improved compared to free GRb1.https://doi.org/10.1038/s41598-023-45858-x |
| spellingShingle | Lixin Du Huiling Lu Yifei Xiao Zhihua Guo Ya Li Preparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticles Scientific Reports |
| title | Preparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticles |
| title_full | Preparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticles |
| title_fullStr | Preparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticles |
| title_full_unstemmed | Preparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticles |
| title_short | Preparation, characterization and in vivo pharmacokinetic study of ginsenoside Rb1-PLGA nanoparticles |
| title_sort | preparation characterization and in vivo pharmacokinetic study of ginsenoside rb1 plga nanoparticles |
| url | https://doi.org/10.1038/s41598-023-45858-x |
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