Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose
Vinegar residue is a key secondary waste in the brewing industry that is often disposed irresponsibly, due to its large quantity and lack of reasonably effective use, causing environmental pollution issues. NaOH was used to pretreat Chinese vinegar residue, and the reaction products were consumed by...
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Format: | Article |
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Czech Academy of Agricultural Sciences
2021-06-01
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Series: | Czech Journal of Food Sciences |
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Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-202103-0002_enzymatic-degradation-of-polysaccharides-in-chinese-vinegar-residue-to-produce-alcohol-and-xylose.php |
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author | Kunyi Liu Shulai Zhou Qi Wang Bin Jiang Liran Yang Bing Lu Huawei Yuan |
author_facet | Kunyi Liu Shulai Zhou Qi Wang Bin Jiang Liran Yang Bing Lu Huawei Yuan |
author_sort | Kunyi Liu |
collection | DOAJ |
description | Vinegar residue is a key secondary waste in the brewing industry that is often disposed irresponsibly, due to its large quantity and lack of reasonably effective use, causing environmental pollution issues. NaOH was used to pretreat Chinese vinegar residue, and the reaction products were consumed by the enzyme complex and Saccharomyces cerevisiae 1300 during the stage of simultaneous saccharification and fermentation (SSF). The results show that the optimal pretreatment conditions for Chinese vinegar residue were solid-to-liquid ratio of 1 : 11, NaOH concentration of 2.2%, pretreatment temperature of 63 °C, pretreatment time of 80 min, and amount of 4.9 IU g-1 xylanase. While these optimal conditions allowed more effective enzymatic degradation of the dried vinegar residue and resulted in the total sugar yield of 66.1%. Subsequently, dried vinegar residue and enzyme complex were added into the SSF process four times, and SSF reacted in a shaker at 120 r min-1 and 37 °C for 120 h, the yields of ethanol and xylose were 31.4% and 18.5%, respectively. Therefore, the method of Chinese vinegar residue for alcohol and xylose production by SSF was proved. |
first_indexed | 2024-04-10T08:32:00Z |
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institution | Directory Open Access Journal |
issn | 1212-1800 1805-9317 |
language | English |
last_indexed | 2024-04-10T08:32:00Z |
publishDate | 2021-06-01 |
publisher | Czech Academy of Agricultural Sciences |
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series | Czech Journal of Food Sciences |
spelling | doaj.art-cb9be973bdc84082bdb1a53723bc5cf12023-02-23T03:28:46ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172021-06-0139316016810.17221/326/2019-CJFScjf-202103-0002Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xyloseKunyi Liu0Shulai Zhou1Qi Wang2Bin Jiang3Liran Yang4Bing Lu5Huawei Yuan6College of Wuliangye Technology and Food Engineering, Yibin Vocational and Technical College, Yibin, ChinaDepartment of Pharmacy, Leshan Vocational and Technical College, Leshan, ChinaCollege of Wuliangye Technology and Food Engineering, Yibin Vocational and Technical College, Yibin, ChinaCollege of Modern Agriculture, Yibin Vocational and Technical College, Yibin, ChinaCollege of Modern Agriculture, Yibin Vocational and Technical College, Yibin, ChinaCollege of Wuliangye Technology and Food Engineering, Yibin Vocational and Technical College, Yibin, China‡College of Life Science and Food Engineering, Yibin University, Yibin, ChinaVinegar residue is a key secondary waste in the brewing industry that is often disposed irresponsibly, due to its large quantity and lack of reasonably effective use, causing environmental pollution issues. NaOH was used to pretreat Chinese vinegar residue, and the reaction products were consumed by the enzyme complex and Saccharomyces cerevisiae 1300 during the stage of simultaneous saccharification and fermentation (SSF). The results show that the optimal pretreatment conditions for Chinese vinegar residue were solid-to-liquid ratio of 1 : 11, NaOH concentration of 2.2%, pretreatment temperature of 63 °C, pretreatment time of 80 min, and amount of 4.9 IU g-1 xylanase. While these optimal conditions allowed more effective enzymatic degradation of the dried vinegar residue and resulted in the total sugar yield of 66.1%. Subsequently, dried vinegar residue and enzyme complex were added into the SSF process four times, and SSF reacted in a shaker at 120 r min-1 and 37 °C for 120 h, the yields of ethanol and xylose were 31.4% and 18.5%, respectively. Therefore, the method of Chinese vinegar residue for alcohol and xylose production by SSF was proved.https://cjfs.agriculturejournals.cz/artkey/cjf-202103-0002_enzymatic-degradation-of-polysaccharides-in-chinese-vinegar-residue-to-produce-alcohol-and-xylose.phpnaohpretreatmentenzymolysissimultaneous saccharification and fermentationxylanaseenzyme complex |
spellingShingle | Kunyi Liu Shulai Zhou Qi Wang Bin Jiang Liran Yang Bing Lu Huawei Yuan Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose Czech Journal of Food Sciences naoh pretreatment enzymolysis simultaneous saccharification and fermentation xylanase enzyme complex |
title | Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose |
title_full | Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose |
title_fullStr | Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose |
title_full_unstemmed | Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose |
title_short | Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose |
title_sort | enzymatic degradation of polysaccharides in chinese vinegar residue to produce alcohol and xylose |
topic | naoh pretreatment enzymolysis simultaneous saccharification and fermentation xylanase enzyme complex |
url | https://cjfs.agriculturejournals.cz/artkey/cjf-202103-0002_enzymatic-degradation-of-polysaccharides-in-chinese-vinegar-residue-to-produce-alcohol-and-xylose.php |
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