Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle disease
Immunoglobulin-A (IgA) response to Newcastle diseases virus (NDV) vaccination was assessed using enzyme-linked immunosorbent assay. Total and NDV specific IgA levels were determined in tracheal washings, intestinal washings and bile. Chickens were primed with an in ovo recombinant avian adeno-associ...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia
2010-05-01
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| Series: | Revista Científica |
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| Online Access: | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/15279 |
| _version_ | 1811163195928215552 |
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| author | Francisco Perozo Giovanny Finol Yaneth Mavárez |
| author_facet | Francisco Perozo Giovanny Finol Yaneth Mavárez |
| author_sort | Francisco Perozo |
| collection | DOAJ |
| description | Immunoglobulin-A (IgA) response to Newcastle diseases virus (NDV) vaccination was assessed using enzyme-linked immunosorbent assay. Total and NDV specific IgA levels were determined in tracheal washings, intestinal washings and bile. Chickens were primed with an in ovo recombinant avian adeno-associated vaccine coding for the NDV hemagglutinin-neuraminidase gene (rAAAV) and revaccinated with B1 or La-Sota strains of the virus. The most suitable samples for IgA determinations were tracheal washings and bile. No NDV specific IgA response was elicited by the rAAAV alone. After revaccination with live NDV virus the IgA levels in the biological samples were significantly higher (P menor 0.005) than the levels in the unvaccinated controls. No strain dependant differences were observed in the NDV specific response after revaccination. Nodifferences in total (unspecific) IgA levels between control and vaccinated birds were observed. In view of the importance of mucosal immune response to NDV infection, the assessment of local antibody levels can aid in the study of the host antiviral response.
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| first_indexed | 2024-04-10T06:41:07Z |
| format | Article |
| id | doaj.art-cb9dacd359c14c7e8c5809c816642671 |
| institution | Directory Open Access Journal |
| issn | 0798-2259 2521-9715 |
| language | English |
| last_indexed | 2024-04-10T06:41:07Z |
| publishDate | 2010-05-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Científica |
| spelling | doaj.art-cb9dacd359c14c7e8c5809c8166426712023-02-28T15:30:10ZengUniversidad del ZuliaRevista Científica0798-22592521-97152010-05-01173Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle diseaseFrancisco Perozo0Giovanny Finol1Yaneth Mavárez2Universidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversity of Georgia-USAImmunoglobulin-A (IgA) response to Newcastle diseases virus (NDV) vaccination was assessed using enzyme-linked immunosorbent assay. Total and NDV specific IgA levels were determined in tracheal washings, intestinal washings and bile. Chickens were primed with an in ovo recombinant avian adeno-associated vaccine coding for the NDV hemagglutinin-neuraminidase gene (rAAAV) and revaccinated with B1 or La-Sota strains of the virus. The most suitable samples for IgA determinations were tracheal washings and bile. No NDV specific IgA response was elicited by the rAAAV alone. After revaccination with live NDV virus the IgA levels in the biological samples were significantly higher (P menor 0.005) than the levels in the unvaccinated controls. No strain dependant differences were observed in the NDV specific response after revaccination. Nodifferences in total (unspecific) IgA levels between control and vaccinated birds were observed. In view of the importance of mucosal immune response to NDV infection, the assessment of local antibody levels can aid in the study of the host antiviral response. http://www.produccioncientificaluz.org/index.php/cientifica/article/view/15279newcastle disease virusimmunoglobulin Aavian adeno-associated virus |
| spellingShingle | Francisco Perozo Giovanny Finol Yaneth Mavárez Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle disease Revista Científica newcastle disease virus immunoglobulin A avian adeno-associated virus |
| title | Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle disease |
| title_full | Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle disease |
| title_fullStr | Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle disease |
| title_full_unstemmed | Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle disease |
| title_short | Levels of immunoglobulin”“A in trachea, gut and bile samples of chickens vaccinated against newcastle disease |
| title_sort | levels of immunoglobulin a in trachea gut and bile samples of chickens vaccinated against newcastle disease |
| topic | newcastle disease virus immunoglobulin A avian adeno-associated virus |
| url | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/15279 |
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