Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i>
Melatonin is a bioactive compound that is present in fermented beverages and has been described to be synthesized by yeast during alcoholic fermentation. The aim of this study was to assess the capacity of intracellular and extracellular melatonin production by different <i>Saccharomyces</i...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/853 |
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| author | María Ángeles Morcillo-Parra Gemma Beltran Albert Mas María-Jesús Torija |
| author_facet | María Ángeles Morcillo-Parra Gemma Beltran Albert Mas María-Jesús Torija |
| author_sort | María Ángeles Morcillo-Parra |
| collection | DOAJ |
| description | Melatonin is a bioactive compound that is present in fermented beverages and has been described to be synthesized by yeast during alcoholic fermentation. The aim of this study was to assess the capacity of intracellular and extracellular melatonin production by different <i>Saccharomyces</i> strains from diverse food origin and to study the effects of different fermentation parameters, such as sugar and nitrogen concentration, temperature or initial population, on melatonin production using a synthetic grape must medium. Melatonin from fermentation samples was analyzed by liquid chromatography mass spectrometry. Intracellular melatonin synthesis profile did not present differences between yeast strains. However, extracellular melatonin production depended on the yeast origin. Thus, we suggest that melatonin production and secretion during the different yeast growth phases follows a species-specific pattern. Other parameters that affected the fermentation process such as sugar content and low temperature had an impact on intracellular melatonin production profile, as well as the melatonin content within the cell. This study reports the effect of several conditions on the melatonin synthesis profile, highlighting its possible role as a signal molecule. |
| first_indexed | 2024-03-10T19:21:15Z |
| format | Article |
| id | doaj.art-cba24590133244d1a34de7e4178f5d1a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:21:15Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cba24590133244d1a34de7e4178f5d1a2023-11-20T02:57:47ZengMDPI AGMicroorganisms2076-26072020-06-018685310.3390/microorganisms8060853Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i>María Ángeles Morcillo-Parra0Gemma Beltran1Albert Mas2María-Jesús Torija3Grup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Universitat Rovira i Virgili, c/Marcel·lí Domingo, 1-43007 Tarragona, SpainGrup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Universitat Rovira i Virgili, c/Marcel·lí Domingo, 1-43007 Tarragona, SpainGrup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Universitat Rovira i Virgili, c/Marcel·lí Domingo, 1-43007 Tarragona, SpainGrup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Universitat Rovira i Virgili, c/Marcel·lí Domingo, 1-43007 Tarragona, SpainMelatonin is a bioactive compound that is present in fermented beverages and has been described to be synthesized by yeast during alcoholic fermentation. The aim of this study was to assess the capacity of intracellular and extracellular melatonin production by different <i>Saccharomyces</i> strains from diverse food origin and to study the effects of different fermentation parameters, such as sugar and nitrogen concentration, temperature or initial population, on melatonin production using a synthetic grape must medium. Melatonin from fermentation samples was analyzed by liquid chromatography mass spectrometry. Intracellular melatonin synthesis profile did not present differences between yeast strains. However, extracellular melatonin production depended on the yeast origin. Thus, we suggest that melatonin production and secretion during the different yeast growth phases follows a species-specific pattern. Other parameters that affected the fermentation process such as sugar content and low temperature had an impact on intracellular melatonin production profile, as well as the melatonin content within the cell. This study reports the effect of several conditions on the melatonin synthesis profile, highlighting its possible role as a signal molecule.https://www.mdpi.com/2076-2607/8/6/853melatoninalcoholic fermentationyeasttemperature<i>Saccharomyces cerevisiae</i> |
| spellingShingle | María Ángeles Morcillo-Parra Gemma Beltran Albert Mas María-Jesús Torija Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i> Microorganisms melatonin alcoholic fermentation yeast temperature <i>Saccharomyces cerevisiae</i> |
| title | Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i> |
| title_full | Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i> |
| title_short | Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in <i>Saccharomyces cerevisiae</i> |
| title_sort | effect of several nutrients and environmental conditions on intracellular melatonin synthesis in i saccharomyces cerevisiae i |
| topic | melatonin alcoholic fermentation yeast temperature <i>Saccharomyces cerevisiae</i> |
| url | https://www.mdpi.com/2076-2607/8/6/853 |
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