Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction
Methicillin-susceptible (MSSA) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a pathogen commonly found in bone and joint infections, including septic arthritis. <i>S. aureus</i> virulence and the frailty of affected patients can cause several complications;...
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
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| author | Viviana Cafiso Flavia Lo Verde Alessandra Zega Giuseppe Pigola Roberto Rostagno Silvio Borrè Stefania Stefani |
| author_facet | Viviana Cafiso Flavia Lo Verde Alessandra Zega Giuseppe Pigola Roberto Rostagno Silvio Borrè Stefania Stefani |
| author_sort | Viviana Cafiso |
| collection | DOAJ |
| description | Methicillin-susceptible (MSSA) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a pathogen commonly found in bone and joint infections, including septic arthritis. <i>S. aureus</i> virulence and the frailty of affected patients can cause several complications; a prompt and specific antibiotic treatment can positively affect the outcome of patients. We carried out an in-depth genomic characterization by Illumina whole genome sequencing and bioinformatics of two biofilm-producing M1 and M2 ST398 MSSA causing septic knee arthritis not-responding to antimicrobial therapy. The strains were characterized for antibiotic resistance, biofilm and adhesive properties as well as genomics, single nucleotide polymorphism phylogeny, resistomics and virulomics. Our results showed that M1 and M2 MSSA were ST398-t1451-<i>agr</i>I-Cap5, susceptible to cefoxitin and resistant to erythromycin and clindamycin, traits consistent with the lack of the SCC<i>mec</i>-locus and the presence of the sole <i>bla</i>Z and <i>erm</i>T. Furthermore, M1 and M2 were biofilm-producing and largely potentially adhesive strains, as indicated by the adhesion gene profile. Our data characterized a new human-adapted ST398 MSSA lineage, representing a “fusion” between the human-animal independent ST398 and the Livestock Associated (LA) ST398 lineages, forming biofilm and genomically predicted high adhesive, characterized by different genomic adaptation conferring a great ability to adhere to the host’s extracellular matrix causing septic knee arthritis. |
| first_indexed | 2024-03-09T06:04:45Z |
| format | Article |
| id | doaj.art-cba293b84bc44a998ef9bfcb942c9f10 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:04:45Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cba293b84bc44a998ef9bfcb942c9f102023-12-03T12:05:12ZengMDPI AGMicroorganisms2076-26072021-02-019230510.3390/microorganisms9020305Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical ReconstructionViviana Cafiso0Flavia Lo Verde1Alessandra Zega2Giuseppe Pigola3Roberto Rostagno4Silvio Borrè5Stefania Stefani6Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyInfectious Diseases Department of Sant’Andrea Hospital Vercelli, 13100 Vercelli, ItalyInfectious Diseases Department of Sant’Andrea Hospital Vercelli, 13100 Vercelli, ItalyDepartment of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyMethicillin-susceptible (MSSA) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a pathogen commonly found in bone and joint infections, including septic arthritis. <i>S. aureus</i> virulence and the frailty of affected patients can cause several complications; a prompt and specific antibiotic treatment can positively affect the outcome of patients. We carried out an in-depth genomic characterization by Illumina whole genome sequencing and bioinformatics of two biofilm-producing M1 and M2 ST398 MSSA causing septic knee arthritis not-responding to antimicrobial therapy. The strains were characterized for antibiotic resistance, biofilm and adhesive properties as well as genomics, single nucleotide polymorphism phylogeny, resistomics and virulomics. Our results showed that M1 and M2 MSSA were ST398-t1451-<i>agr</i>I-Cap5, susceptible to cefoxitin and resistant to erythromycin and clindamycin, traits consistent with the lack of the SCC<i>mec</i>-locus and the presence of the sole <i>bla</i>Z and <i>erm</i>T. Furthermore, M1 and M2 were biofilm-producing and largely potentially adhesive strains, as indicated by the adhesion gene profile. Our data characterized a new human-adapted ST398 MSSA lineage, representing a “fusion” between the human-animal independent ST398 and the Livestock Associated (LA) ST398 lineages, forming biofilm and genomically predicted high adhesive, characterized by different genomic adaptation conferring a great ability to adhere to the host’s extracellular matrix causing septic knee arthritis.https://www.mdpi.com/2076-2607/9/2/305biofilmadhesionST398<i>S. aureus</i>genomics |
| spellingShingle | Viviana Cafiso Flavia Lo Verde Alessandra Zega Giuseppe Pigola Roberto Rostagno Silvio Borrè Stefania Stefani Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction Microorganisms biofilm adhesion ST398 <i>S. aureus</i> genomics |
| title | Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction |
| title_full | Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction |
| title_fullStr | Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction |
| title_full_unstemmed | Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction |
| title_short | Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction |
| title_sort | genomic characterization of a new biofilm forming and adhesive st398 human adapted mssa lineage causing septic knee arthritis following surgical reconstruction |
| topic | biofilm adhesion ST398 <i>S. aureus</i> genomics |
| url | https://www.mdpi.com/2076-2607/9/2/305 |
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