Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq
Summary: Ribosome profiling (Ribo-seq) measures ribosome density along messenger RNA (mRNA) transcripts and is used to estimate the “translational fitness” of a given mRNA in response to environmental or developmental cues with high resolution. Here, we describe a protocol for Ribo-seq in plants ada...
Main Authors: | Isabel Cristina Vélez-Bermúdez, Shu-Jen Chou, Ai-Ping Chen, Wen-Dar Lin, Wolfgang Schmidt |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-09-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004872 |
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