Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
Abstract Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a g...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-020-00760-6 |
| _version_ | 1819060148877918208 |
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| author | Simon Menanteau-Ledouble Oskar Schachner Mark L. Lawrence Mansour El-Matbouli |
| author_facet | Simon Menanteau-Ledouble Oskar Schachner Mark L. Lawrence Mansour El-Matbouli |
| author_sort | Simon Menanteau-Ledouble |
| collection | DOAJ |
| description | Abstract Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamicin assay to quantify the effect of silencing on the cells’ susceptibility to infection and found that silencing of 18 out of 20 genes significantly reduced the number of Y. ruckeri recovered. These findings improve our understanding of the infection process by Y. ruckeri and of the interactions between this bacterial pathogen and host cells. |
| first_indexed | 2024-12-21T14:22:23Z |
| format | Article |
| id | doaj.art-cba398d7d8bc47ef9e8bb5607fc1f7d7 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-21T14:22:23Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-cba398d7d8bc47ef9e8bb5607fc1f7d72022-12-21T19:00:45ZengBMCVeterinary Research1297-97162020-03-015111710.1186/s13567-020-00760-6Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeriSimon Menanteau-Ledouble0Oskar Schachner1Mark L. Lawrence2Mansour El-Matbouli3Clinical Division of Fish Medicine, University of Veterinary MedicineClinical Division of Fish Medicine, University of Veterinary MedicineFeed the Future Fish Innovation Lab for FishClinical Division of Fish Medicine, University of Veterinary MedicineAbstract Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamicin assay to quantify the effect of silencing on the cells’ susceptibility to infection and found that silencing of 18 out of 20 genes significantly reduced the number of Y. ruckeri recovered. These findings improve our understanding of the infection process by Y. ruckeri and of the interactions between this bacterial pathogen and host cells.http://link.springer.com/article/10.1186/s13567-020-00760-6 |
| spellingShingle | Simon Menanteau-Ledouble Oskar Schachner Mark L. Lawrence Mansour El-Matbouli Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri Veterinary Research |
| title | Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri |
| title_full | Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri |
| title_fullStr | Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri |
| title_full_unstemmed | Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri |
| title_short | Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri |
| title_sort | effects of sirna silencing on the susceptibility of the fish cell line chse 214 to yersinia ruckeri |
| url | http://link.springer.com/article/10.1186/s13567-020-00760-6 |
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