Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle
Abstract Hemoglobin (Hb) is a powerful promoter of lipid oxidation, particularly in muscle of small pelagic fish species and fish by-products, both having high Hb-levels and highly unsaturated lipids. As Hb is located within the red blood cells (RBCs) it is here hypothesized that the perishable poly...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16363-4 |
| _version_ | 1818505159115800576 |
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| author | Semhar Ghirmai Haizhou Wu Michael Axelsson Takashi Matsuhira Hiromi Sakai Ingrid Undeland |
| author_facet | Semhar Ghirmai Haizhou Wu Michael Axelsson Takashi Matsuhira Hiromi Sakai Ingrid Undeland |
| author_sort | Semhar Ghirmai |
| collection | DOAJ |
| description | Abstract Hemoglobin (Hb) is a powerful promoter of lipid oxidation, particularly in muscle of small pelagic fish species and fish by-products, both having high Hb-levels and highly unsaturated lipids. As Hb is located within the red blood cells (RBCs) it is here hypothesized that the perishable polyunsaturated fatty acids (PUFAs) can be protected from oxidation by limiting hemolysis during early fish processing. Using a model system consisting of washed-resuspended trout (Oncorhynchus mykiss) RBCs (wr-RBCs), the aim of this study was to evaluate how RBC lysis under cold storage was affected by selected parameters linked to blood or muscle: bacterial growth, energy status, pH, RBC membrane lipid oxidation and colloidal osmotic pressure (COP). The results indicated that bacterial growth had a modest effect on hemolysis while pH-values typical for post mortem fish muscle (6.4–6.8), and absence of glucose or albumin stimulated hemolysis. The rapid hemolysis observed at pH 6.4–6.8 correlated with lipid oxidation of the RBC membrane, while the lower hemolysis at pH 7.2–8.0 occurred with low, or without any RBC membrane lipid oxidation. When hemin was added to the RBCs at pH 6.8 hemolysis was induced without parallel RBC membrane oxidation, pointing at Hb-autoxidation and hemin-release per se as important events triggering lysis in fish muscle. Altogether, the study provided valuable findings which ultimately can aid development of new tools to combat lipid oxidation in post mortem fish muscle by limiting hemolysis. |
| first_indexed | 2024-12-10T21:46:59Z |
| format | Article |
| id | doaj.art-cba4599a9bc74fb6807d99eda4d69231 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T21:46:59Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cba4599a9bc74fb6807d99eda4d692312022-12-22T01:32:20ZengNature PortfolioScientific Reports2045-23222022-08-0112111010.1038/s41598-022-16363-4Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscleSemhar Ghirmai0Haizhou Wu1Michael Axelsson2Takashi Matsuhira3Hiromi Sakai4Ingrid Undeland5Division of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of TechnologyDivision of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Biological and Environmental Sciences, Gothenburg UniversityDepartment of Chemistry, Nara Medical UniversityDepartment of Chemistry, Nara Medical UniversityDivision of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of TechnologyAbstract Hemoglobin (Hb) is a powerful promoter of lipid oxidation, particularly in muscle of small pelagic fish species and fish by-products, both having high Hb-levels and highly unsaturated lipids. As Hb is located within the red blood cells (RBCs) it is here hypothesized that the perishable polyunsaturated fatty acids (PUFAs) can be protected from oxidation by limiting hemolysis during early fish processing. Using a model system consisting of washed-resuspended trout (Oncorhynchus mykiss) RBCs (wr-RBCs), the aim of this study was to evaluate how RBC lysis under cold storage was affected by selected parameters linked to blood or muscle: bacterial growth, energy status, pH, RBC membrane lipid oxidation and colloidal osmotic pressure (COP). The results indicated that bacterial growth had a modest effect on hemolysis while pH-values typical for post mortem fish muscle (6.4–6.8), and absence of glucose or albumin stimulated hemolysis. The rapid hemolysis observed at pH 6.4–6.8 correlated with lipid oxidation of the RBC membrane, while the lower hemolysis at pH 7.2–8.0 occurred with low, or without any RBC membrane lipid oxidation. When hemin was added to the RBCs at pH 6.8 hemolysis was induced without parallel RBC membrane oxidation, pointing at Hb-autoxidation and hemin-release per se as important events triggering lysis in fish muscle. Altogether, the study provided valuable findings which ultimately can aid development of new tools to combat lipid oxidation in post mortem fish muscle by limiting hemolysis.https://doi.org/10.1038/s41598-022-16363-4 |
| spellingShingle | Semhar Ghirmai Haizhou Wu Michael Axelsson Takashi Matsuhira Hiromi Sakai Ingrid Undeland Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle Scientific Reports |
| title | Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle |
| title_full | Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle |
| title_fullStr | Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle |
| title_full_unstemmed | Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle |
| title_short | Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle |
| title_sort | exploring how plasma and muscle related parameters affect trout hemolysis as a route to prevent hemoglobin mediated lipid oxidation of fish muscle |
| url | https://doi.org/10.1038/s41598-022-16363-4 |
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