Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells
Host plants excrete a glucosylation enzyme onto the plant surface that changes mycotoxins derived from fungal secondary metabolites to glucosylated products. Deoxynivalenol-3-glucoside (DON3G) is synthesized by grain uridine diphosphate-glucosyltransferase, and is found worldwide, although inform...
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-01-01
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| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/7/1/187 |
| _version_ | 1798040156442198016 |
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| author | Tadahiro Suzuki Yumiko Iwahashi |
| author_facet | Tadahiro Suzuki Yumiko Iwahashi |
| author_sort | Tadahiro Suzuki |
| collection | DOAJ |
| description | Host plants excrete a glucosylation enzyme onto the plant surface that changes mycotoxins derived from fungal secondary metabolites to glucosylated products. Deoxynivalenol-3-glucoside (DON3G) is synthesized by grain uridine diphosphate-glucosyltransferase, and is found worldwide, although information on its toxicity is lacking. Here, we conducted growth tests and DNA microarray analysis to elucidate the characteristics of DON3G. The Saccharomyces cerevisiae PDR5 mutant strain exposed to DON3G demonstrated similar growth to the dimethyl sulfoxide control, and DNA microarray analysis revealed limited differences. Only 10 genes were extracted, and the expression profile of stress response genes was similar to that of DON, in contrast to metabolism genes like SER3, which encodes 3-phosphoglycerate dehydrogenase. Growth tests with Chlamydomonas reinhardtii also showed a similar growth rate to the control sample. These results suggest that DON3G has extremely low toxicity to these cells, and the glucosylation of mycotoxins is a useful protective mechanism not only for host plants, but also for other species. |
| first_indexed | 2024-04-11T22:03:34Z |
| format | Article |
| id | doaj.art-cba5a1bb6ce8483880def769373207a3 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T22:03:34Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-cba5a1bb6ce8483880def769373207a32022-12-22T04:00:48ZengMDPI AGToxins2072-66512015-01-017118720010.3390/toxins7010187toxins7010187Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial CellsTadahiro Suzuki0Yumiko Iwahashi1Applied Microbiology Division, National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba, Ibaraki 305-8642, JapanApplied Microbiology Division, National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba, Ibaraki 305-8642, JapanHost plants excrete a glucosylation enzyme onto the plant surface that changes mycotoxins derived from fungal secondary metabolites to glucosylated products. Deoxynivalenol-3-glucoside (DON3G) is synthesized by grain uridine diphosphate-glucosyltransferase, and is found worldwide, although information on its toxicity is lacking. Here, we conducted growth tests and DNA microarray analysis to elucidate the characteristics of DON3G. The Saccharomyces cerevisiae PDR5 mutant strain exposed to DON3G demonstrated similar growth to the dimethyl sulfoxide control, and DNA microarray analysis revealed limited differences. Only 10 genes were extracted, and the expression profile of stress response genes was similar to that of DON, in contrast to metabolism genes like SER3, which encodes 3-phosphoglycerate dehydrogenase. Growth tests with Chlamydomonas reinhardtii also showed a similar growth rate to the control sample. These results suggest that DON3G has extremely low toxicity to these cells, and the glucosylation of mycotoxins is a useful protective mechanism not only for host plants, but also for other species.http://www.mdpi.com/2072-6651/7/1/187deoxynivalenol-3-glucosideDNA microarrayyeastChlamydomonas reinhardtii |
| spellingShingle | Tadahiro Suzuki Yumiko Iwahashi Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells Toxins deoxynivalenol-3-glucoside DNA microarray yeast Chlamydomonas reinhardtii |
| title | Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells |
| title_full | Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells |
| title_fullStr | Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells |
| title_full_unstemmed | Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells |
| title_short | Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells |
| title_sort | low toxicity of deoxynivalenol 3 glucoside in microbial cells |
| topic | deoxynivalenol-3-glucoside DNA microarray yeast Chlamydomonas reinhardtii |
| url | http://www.mdpi.com/2072-6651/7/1/187 |
| work_keys_str_mv | AT tadahirosuzuki lowtoxicityofdeoxynivalenol3glucosideinmicrobialcells AT yumikoiwahashi lowtoxicityofdeoxynivalenol3glucosideinmicrobialcells |