The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein.
Chemical chaperones are small organic molecules which accumulate in a broad range of organisms in various tissues under different stress conditions and assist in the maintenance of a correct proteostasis under denaturating environments. The effect of chemical chaperones on protein folding and aggreg...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3618117?pdf=render |
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| author | Ayala Lampel Yaron Bram Michal Levy-Sakin Eran Bacharach Ehud Gazit |
| author_facet | Ayala Lampel Yaron Bram Michal Levy-Sakin Eran Bacharach Ehud Gazit |
| author_sort | Ayala Lampel |
| collection | DOAJ |
| description | Chemical chaperones are small organic molecules which accumulate in a broad range of organisms in various tissues under different stress conditions and assist in the maintenance of a correct proteostasis under denaturating environments. The effect of chemical chaperones on protein folding and aggregation has been extensively studied and is generally considered to be mediated through non-specific interactions. However, the precise mechanism of action remains elusive. Protein self-assembly is a key event in both native and pathological states, ranging from microtubules and actin filaments formation to toxic amyloids appearance in degenerative disorders, such as Alzheimer's and Parkinson's diseases. Another pathological event, in which protein assembly cascade is a fundamental process, is the formation of virus particles. In the late stage of the virus life cycle, capsid proteins self-assemble into highly-ordered cores, which encapsulate the viral genome, consequently protect genome integrity and mediate infectivity. In this study, we examined the effect of different groups of chemical chaperones on viral capsid assembly in vitro, focusing on HIV-1 capsid protein as a system model. We found that while polyols and sugars markedly inhibited capsid assembly, methylamines dramatically enhanced the assembly rate. Moreover, chemical chaperones that inhibited capsid core formation, also stabilized capsid structure under thermal denaturation. Correspondingly, trimethylamine N-oxide, which facilitated formation of high-order assemblies, clearly destabilized capsid structure under similar conditions. In contrast to the prevailing hypothesis suggesting that chemical chaperones affect proteins through preferential exclusion, the observed dual effects imply that different chaperones modify capsid assembly and stability through different mechanisms. Furthermore, our results indicate a correlation between the folding state of capsid to its tendency to assemble into highly-ordered structures. |
| first_indexed | 2024-12-20T05:22:09Z |
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| id | doaj.art-cba61021cc0b4fb3876eb4cf15c7a692 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T05:22:09Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cba61021cc0b4fb3876eb4cf15c7a6922022-12-21T19:52:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6086710.1371/journal.pone.0060867The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein.Ayala LampelYaron BramMichal Levy-SakinEran BacharachEhud GazitChemical chaperones are small organic molecules which accumulate in a broad range of organisms in various tissues under different stress conditions and assist in the maintenance of a correct proteostasis under denaturating environments. The effect of chemical chaperones on protein folding and aggregation has been extensively studied and is generally considered to be mediated through non-specific interactions. However, the precise mechanism of action remains elusive. Protein self-assembly is a key event in both native and pathological states, ranging from microtubules and actin filaments formation to toxic amyloids appearance in degenerative disorders, such as Alzheimer's and Parkinson's diseases. Another pathological event, in which protein assembly cascade is a fundamental process, is the formation of virus particles. In the late stage of the virus life cycle, capsid proteins self-assemble into highly-ordered cores, which encapsulate the viral genome, consequently protect genome integrity and mediate infectivity. In this study, we examined the effect of different groups of chemical chaperones on viral capsid assembly in vitro, focusing on HIV-1 capsid protein as a system model. We found that while polyols and sugars markedly inhibited capsid assembly, methylamines dramatically enhanced the assembly rate. Moreover, chemical chaperones that inhibited capsid core formation, also stabilized capsid structure under thermal denaturation. Correspondingly, trimethylamine N-oxide, which facilitated formation of high-order assemblies, clearly destabilized capsid structure under similar conditions. In contrast to the prevailing hypothesis suggesting that chemical chaperones affect proteins through preferential exclusion, the observed dual effects imply that different chaperones modify capsid assembly and stability through different mechanisms. Furthermore, our results indicate a correlation between the folding state of capsid to its tendency to assemble into highly-ordered structures.http://europepmc.org/articles/PMC3618117?pdf=render |
| spellingShingle | Ayala Lampel Yaron Bram Michal Levy-Sakin Eran Bacharach Ehud Gazit The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein. PLoS ONE |
| title | The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein. |
| title_full | The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein. |
| title_fullStr | The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein. |
| title_full_unstemmed | The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein. |
| title_short | The effect of chemical chaperones on the assembly and stability of HIV-1 capsid protein. |
| title_sort | effect of chemical chaperones on the assembly and stability of hiv 1 capsid protein |
| url | http://europepmc.org/articles/PMC3618117?pdf=render |
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