Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats
Abstract Lablab purpureus (L) bean is an undervalued or underutilized orphan crop in many tropical countries, where different forms of malnutrition are associated with stunting growth and cognitive deficiencies. We previously reported that L. purpureus contains ω‐3 α‐linolenic acid (ALA, C18:3, ω‐3)...
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| Format: | Article |
| Language: | English |
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Wiley
2020-06-01
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| Series: | Legume Science |
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| Online Access: | https://doi.org/10.1002/leg3.34 |
| _version_ | 1818478906948190208 |
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| author | Shahdat Hossain Rashed Ahmed Sujan Bhowmick Marzan Sarkar Taslima Nahar Borhan Uddin Mafroz Basunia Abdullah Al Mamun Michio Hashimoto Osamu Shido |
| author_facet | Shahdat Hossain Rashed Ahmed Sujan Bhowmick Marzan Sarkar Taslima Nahar Borhan Uddin Mafroz Basunia Abdullah Al Mamun Michio Hashimoto Osamu Shido |
| author_sort | Shahdat Hossain |
| collection | DOAJ |
| description | Abstract Lablab purpureus (L) bean is an undervalued or underutilized orphan crop in many tropical countries, where different forms of malnutrition are associated with stunting growth and cognitive deficiencies. We previously reported that L. purpureus contains ω‐3 α‐linolenic acid (ALA, C18:3, ω‐3), which can act as the precursor of ω‐3 docosahexaenoic acid (DHA, C22:6, ω‐3). Inadequate level of DHA impairs growth, development, and cognitive performance. Therefore, we evaluated if supplementation of L. purpureus seed flour (LPS) affects the nutritional status, in terms of body weight gain, plasma proteins, and DHA levels of malnourished model rats. Three groups of rats, namely, controls, malnourished alone (MN), and 15% LPS‐supplemented malnourished (MN + LPS) rats were fed with LPS for 12 weeks. Afterward, body weight, liver weight, brain weight, plasma proteins, micronutrients, lipid profile, and fatty acid profile of plasma, liver, and brains were determined by standard methods. The levels of liver lipid peroxide (LPO) and proinflammatory TNFα were also measured. The body weight, liver weight, serum total proteins and micronutrients (iron/potassium), and the levels of docosahexaenoic acid significantly increased in the plasma, liver, and brain of MN + LPS rats. Moreover, LPO and TNFα levels reduced significantly in MN + LPS rats. in vitro analysis revealed a significant free radical scavenging and antioxidative potential of L. purpureus seed extract. Thus, L. purpureus not only replenishes protein‐energy malnutrition, but also increases the levels of DHA, an indispensable polyunsaturated fatty acid for brain cognition. Finally, our results suggest that L. purpureus might benefit human malnutrition and related cognitive deficits. |
| first_indexed | 2024-12-10T09:54:03Z |
| format | Article |
| id | doaj.art-cba942e150044667aae823248be4f722 |
| institution | Directory Open Access Journal |
| issn | 2639-6181 |
| language | English |
| last_indexed | 2024-12-10T09:54:03Z |
| publishDate | 2020-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Legume Science |
| spelling | doaj.art-cba942e150044667aae823248be4f7222022-12-22T01:53:34ZengWileyLegume Science2639-61812020-06-012218119310.1002/leg3.34Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished ratsShahdat Hossain0Rashed Ahmed1Sujan Bhowmick2Marzan Sarkar3Taslima Nahar4Borhan Uddin5Mafroz Basunia6Abdullah Al Mamun7Michio Hashimoto8Osamu Shido9Department of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences Jahangirnagar University Savar Dhaka BangladeshDepartment of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences Jahangirnagar University Savar Dhaka BangladeshDepartment of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences Jahangirnagar University Savar Dhaka BangladeshDepartment of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences Jahangirnagar University Savar Dhaka BangladeshDepartment of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences Jahangirnagar University Savar Dhaka BangladeshDepartment of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences Jahangirnagar University Savar Dhaka BangladeshDepartment of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences Jahangirnagar University Savar Dhaka BangladeshDepartment of Environmental Physiology, Faculty of Medicine Shimane University Izumo Shimane JapanDepartment of Environmental Physiology, Faculty of Medicine Shimane University Izumo Shimane JapanDepartment of Environmental Physiology, Faculty of Medicine Shimane University Izumo Shimane JapanAbstract Lablab purpureus (L) bean is an undervalued or underutilized orphan crop in many tropical countries, where different forms of malnutrition are associated with stunting growth and cognitive deficiencies. We previously reported that L. purpureus contains ω‐3 α‐linolenic acid (ALA, C18:3, ω‐3), which can act as the precursor of ω‐3 docosahexaenoic acid (DHA, C22:6, ω‐3). Inadequate level of DHA impairs growth, development, and cognitive performance. Therefore, we evaluated if supplementation of L. purpureus seed flour (LPS) affects the nutritional status, in terms of body weight gain, plasma proteins, and DHA levels of malnourished model rats. Three groups of rats, namely, controls, malnourished alone (MN), and 15% LPS‐supplemented malnourished (MN + LPS) rats were fed with LPS for 12 weeks. Afterward, body weight, liver weight, brain weight, plasma proteins, micronutrients, lipid profile, and fatty acid profile of plasma, liver, and brains were determined by standard methods. The levels of liver lipid peroxide (LPO) and proinflammatory TNFα were also measured. The body weight, liver weight, serum total proteins and micronutrients (iron/potassium), and the levels of docosahexaenoic acid significantly increased in the plasma, liver, and brain of MN + LPS rats. Moreover, LPO and TNFα levels reduced significantly in MN + LPS rats. in vitro analysis revealed a significant free radical scavenging and antioxidative potential of L. purpureus seed extract. Thus, L. purpureus not only replenishes protein‐energy malnutrition, but also increases the levels of DHA, an indispensable polyunsaturated fatty acid for brain cognition. Finally, our results suggest that L. purpureus might benefit human malnutrition and related cognitive deficits.https://doi.org/10.1002/leg3.34braindocosahexaenoic acid (DHA)Lablab purpureusmalnutrition |
| spellingShingle | Shahdat Hossain Rashed Ahmed Sujan Bhowmick Marzan Sarkar Taslima Nahar Borhan Uddin Mafroz Basunia Abdullah Al Mamun Michio Hashimoto Osamu Shido Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats Legume Science brain docosahexaenoic acid (DHA) Lablab purpureus malnutrition |
| title | Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats |
| title_full | Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats |
| title_fullStr | Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats |
| title_full_unstemmed | Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats |
| title_short | Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats |
| title_sort | lablab purpureus l bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid dha 22 6 ω 3 in the plasma liver and brain of malnourished rats |
| topic | brain docosahexaenoic acid (DHA) Lablab purpureus malnutrition |
| url | https://doi.org/10.1002/leg3.34 |
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