Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>)
Mannan oligosaccharide (MOS) is prebiotic with high functionality in aquaculture. The current study investigated the potential roles of MOS on the growth performance, digestive enzyme activity, carcass composition, and blood chemistry of Thinlip grey mullet (<i>Liza ramada</i>). Four tes...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/12/3559 |
| _version_ | 1797506968499257344 |
|---|---|
| author | Fawzy I. Magouz Mohamed I. Bassuini Malik M. Khalafalla Ramy Abbas Hani Sewilam Salama Mostafa Aboelenin Mohamed Mohamed Soliman Asem A. Amer Ali A. Soliman Hien Van Doan Mahmoud A. O. Dawood |
| author_facet | Fawzy I. Magouz Mohamed I. Bassuini Malik M. Khalafalla Ramy Abbas Hani Sewilam Salama Mostafa Aboelenin Mohamed Mohamed Soliman Asem A. Amer Ali A. Soliman Hien Van Doan Mahmoud A. O. Dawood |
| author_sort | Fawzy I. Magouz |
| collection | DOAJ |
| description | Mannan oligosaccharide (MOS) is prebiotic with high functionality in aquaculture. The current study investigated the potential roles of MOS on the growth performance, digestive enzyme activity, carcass composition, and blood chemistry of Thinlip grey mullet (<i>Liza ramada</i>). Four tested diets with 34.49% crude protein and 6.29% of total lipids were prepared and fortified with 0, 0.5, 1, and 2% MOS. Fish of initial weight = 5.14 ± 0.11 g/fish were distributed in 12 hapas (0.5 × 0.5 × 1 m) at 15 fish per hapa (triplicates) and fed the test diets to the satiation level two times a day (08:00 and 15:00) for eight weeks. At the end of the trial, all fish were weighed individually for growth performance calculation. Blood was collected to check blood chemistry traits, and intestines were dissected for digestive enzyme analysis. Fish treated with MOS had marked enhancement in the final body weight, feed conversion ratio, protein gain, and protein retention regardless of inclusion dose (<i>p</i> < 0.05). The weight gain, specific growth rate, and protein efficiency ratio were meaningfully enhanced by including MOS at 0.5 and 1%, followed by fish fed with 2% MOS, while the lowest values were in the control group (<i>p</i> < 0.05). Insignificant influences of MOS were seen on the chemical composition of carcass components (moisture, crude protein, total lipids, and ash) (<i>p</i> > 0.05). Fish treated with MOS at 0.5 and 1% had marked enhancement in the amylase, lipase, and protease activities regardless of inclusion dose (<i>p</i> < 0.05). The blood total protein and albumin levels were meaningfully enhanced by including MOS at 0.5 and 1%, followed by fish fed with 2% MOS, while the lowest values were in the control group (<i>p</i> < 0.05). The blood globulin was significantly enhanced in fish fed 1% MOS than fish treated with 0, 0.5, and 2% of MOS (<i>p</i> < 0.05). The blood lysozyme activity was meaningfully enhanced by including MOS at 1%, followed by fish treated with 0.5 and 2%, while the lowest values were in the control group (<i>p</i> < 0.05). Phagocytic activity and phagocytic index were markedly improved in fish treated with 1 and 2% MOS, followed by those fed 0.5% compared with fish fed MOS-free diet (<i>p</i> < 0.05). Superoxide dismutase and glutathione peroxidase were markedly improved in fish treated with 1, and 2% MOS, followed by those fed 0.5% compared with fish fed MOS-free diet (<i>p</i> < 0.05). Dietary MOS (0.5, 1, and 2%) meaningfully enhanced catalase activity while decreased the malondialdehyde concentration (<i>p</i> < 0.05). In summary, dietary MOS is required at 0.5–1% for enhancing the growth rate, feed efficiency, digestive enzyme activity, blood chemistry, and antioxidative capacity of grey mullet. |
| first_indexed | 2024-03-10T04:39:58Z |
| format | Article |
| id | doaj.art-cba99b2a43914a22a6c6adec1fc55e16 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T04:39:58Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-cba99b2a43914a22a6c6adec1fc55e162023-11-23T03:28:51ZengMDPI AGAnimals2076-26152021-12-011112355910.3390/ani11123559Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>)Fawzy I. Magouz0Mohamed I. Bassuini1Malik M. Khalafalla2Ramy Abbas3Hani Sewilam4Salama Mostafa Aboelenin5Mohamed Mohamed Soliman6Asem A. Amer7Ali A. Soliman8Hien Van Doan9Mahmoud A. O. Dawood10Animal Production Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, EgyptAnimal Production Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, EgyptDepartment of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafr El-Sheikh 33516, EgyptAnimal Production Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, EgyptThe Center for Applied Research on the Environment and Sustainability, The American University in Cairo, Cairo 11835, EgyptBiology Department, Turabah University College, Taif University, Taif 21944, Saudi ArabiaClinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21944, Saudi ArabiaCentral Laboratory for Aquaculture Research, Abbassa, Sakha Aquaculture Research Unit, Kafr El-Sheikh 33516, EgyptFish Nutrition Laboratory, Aquaculture Division, National Institute of Oceanography and Fisheries, Alexandria 11865, EgyptDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandAnimal Production Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, EgyptMannan oligosaccharide (MOS) is prebiotic with high functionality in aquaculture. The current study investigated the potential roles of MOS on the growth performance, digestive enzyme activity, carcass composition, and blood chemistry of Thinlip grey mullet (<i>Liza ramada</i>). Four tested diets with 34.49% crude protein and 6.29% of total lipids were prepared and fortified with 0, 0.5, 1, and 2% MOS. Fish of initial weight = 5.14 ± 0.11 g/fish were distributed in 12 hapas (0.5 × 0.5 × 1 m) at 15 fish per hapa (triplicates) and fed the test diets to the satiation level two times a day (08:00 and 15:00) for eight weeks. At the end of the trial, all fish were weighed individually for growth performance calculation. Blood was collected to check blood chemistry traits, and intestines were dissected for digestive enzyme analysis. Fish treated with MOS had marked enhancement in the final body weight, feed conversion ratio, protein gain, and protein retention regardless of inclusion dose (<i>p</i> < 0.05). The weight gain, specific growth rate, and protein efficiency ratio were meaningfully enhanced by including MOS at 0.5 and 1%, followed by fish fed with 2% MOS, while the lowest values were in the control group (<i>p</i> < 0.05). Insignificant influences of MOS were seen on the chemical composition of carcass components (moisture, crude protein, total lipids, and ash) (<i>p</i> > 0.05). Fish treated with MOS at 0.5 and 1% had marked enhancement in the amylase, lipase, and protease activities regardless of inclusion dose (<i>p</i> < 0.05). The blood total protein and albumin levels were meaningfully enhanced by including MOS at 0.5 and 1%, followed by fish fed with 2% MOS, while the lowest values were in the control group (<i>p</i> < 0.05). The blood globulin was significantly enhanced in fish fed 1% MOS than fish treated with 0, 0.5, and 2% of MOS (<i>p</i> < 0.05). The blood lysozyme activity was meaningfully enhanced by including MOS at 1%, followed by fish treated with 0.5 and 2%, while the lowest values were in the control group (<i>p</i> < 0.05). Phagocytic activity and phagocytic index were markedly improved in fish treated with 1 and 2% MOS, followed by those fed 0.5% compared with fish fed MOS-free diet (<i>p</i> < 0.05). Superoxide dismutase and glutathione peroxidase were markedly improved in fish treated with 1, and 2% MOS, followed by those fed 0.5% compared with fish fed MOS-free diet (<i>p</i> < 0.05). Dietary MOS (0.5, 1, and 2%) meaningfully enhanced catalase activity while decreased the malondialdehyde concentration (<i>p</i> < 0.05). In summary, dietary MOS is required at 0.5–1% for enhancing the growth rate, feed efficiency, digestive enzyme activity, blood chemistry, and antioxidative capacity of grey mullet.https://www.mdpi.com/2076-2615/11/12/3559mulletfeed utilizationantioxidant enzyme systemfeed additivesproximate chemical composition |
| spellingShingle | Fawzy I. Magouz Mohamed I. Bassuini Malik M. Khalafalla Ramy Abbas Hani Sewilam Salama Mostafa Aboelenin Mohamed Mohamed Soliman Asem A. Amer Ali A. Soliman Hien Van Doan Mahmoud A. O. Dawood Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>) Animals mullet feed utilization antioxidant enzyme system feed additives proximate chemical composition |
| title | Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>) |
| title_full | Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>) |
| title_fullStr | Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>) |
| title_full_unstemmed | Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>) |
| title_short | Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (<i>Liza ramada</i>) |
| title_sort | mannan oligosaccharide enhanced the growth rate digestive enzyme activity carcass composition and blood chemistry of thinlip grey mullet i liza ramada i |
| topic | mullet feed utilization antioxidant enzyme system feed additives proximate chemical composition |
| url | https://www.mdpi.com/2076-2615/11/12/3559 |
| work_keys_str_mv | AT fawzyimagouz mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT mohamedibassuini mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT malikmkhalafalla mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT ramyabbas mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT hanisewilam mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT salamamostafaaboelenin mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT mohamedmohamedsoliman mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT asemaamer mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT aliasoliman mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT hienvandoan mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai AT mahmoudaodawood mannanoligosaccharideenhancedthegrowthratedigestiveenzymeactivitycarcasscompositionandbloodchemistryofthinlipgreymulletilizaramadai |