Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells
Abstract The human leukemia cell line (HL-60) is an alternative to primary neutrophils in research studies. However, because HL-60 cells proliferate in an incompletely differentiated state, they must undergo differentiation before they acquire the functional properties of neutrophils. Here we provid...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78854-6 |
| _version_ | 1819121216458326016 |
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| author | Kehinde Adebayo Babatunde Xiao Wang Alex Hopke Nils Lannes Pierre-Yves Mantel Daniel Irimia |
| author_facet | Kehinde Adebayo Babatunde Xiao Wang Alex Hopke Nils Lannes Pierre-Yves Mantel Daniel Irimia |
| author_sort | Kehinde Adebayo Babatunde |
| collection | DOAJ |
| description | Abstract The human leukemia cell line (HL-60) is an alternative to primary neutrophils in research studies. However, because HL-60 cells proliferate in an incompletely differentiated state, they must undergo differentiation before they acquire the functional properties of neutrophils. Here we provide evidence of swarming and chemotaxis in differentiated HL-60 neutrophil-like cells (dHL-60) using precise microfluidic assays. We found that dimethyl sulfoxide differentiated HL-60 cells (DdHL-60) have a larger size, increased length, and lower ability to squeeze through narrow channels compared to primary neutrophils. They migrate through tapered microfluidic channels slower than primary neutrophils, but faster than HL-60s differentiated by other protocols, e.g., using all-trans retinoic acid. We found that dHL-60 can swarm toward zymosan particle clusters, though they display disorganized migratory patterns and produce swarms of smaller size compared to primary neutrophils. |
| first_indexed | 2024-12-22T06:33:02Z |
| format | Article |
| id | doaj.art-cbaaead4ca8e4afdb36c67fb26d39d76 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T06:33:02Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cbaaead4ca8e4afdb36c67fb26d39d762022-12-21T18:35:39ZengNature PortfolioScientific Reports2045-23222021-01-0111111310.1038/s41598-020-78854-6Chemotaxis and swarming in differentiated HL-60 neutrophil-like cellsKehinde Adebayo Babatunde0Xiao Wang1Alex Hopke2Nils Lannes3Pierre-Yves Mantel4Daniel Irimia5Department of Surgery, BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical SchoolDepartment of Surgery, BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical SchoolDepartment of Surgery, BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical SchoolDepartment of Medicine, University of FribourgDepartment of Medicine, University of FribourgDepartment of Surgery, BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical SchoolAbstract The human leukemia cell line (HL-60) is an alternative to primary neutrophils in research studies. However, because HL-60 cells proliferate in an incompletely differentiated state, they must undergo differentiation before they acquire the functional properties of neutrophils. Here we provide evidence of swarming and chemotaxis in differentiated HL-60 neutrophil-like cells (dHL-60) using precise microfluidic assays. We found that dimethyl sulfoxide differentiated HL-60 cells (DdHL-60) have a larger size, increased length, and lower ability to squeeze through narrow channels compared to primary neutrophils. They migrate through tapered microfluidic channels slower than primary neutrophils, but faster than HL-60s differentiated by other protocols, e.g., using all-trans retinoic acid. We found that dHL-60 can swarm toward zymosan particle clusters, though they display disorganized migratory patterns and produce swarms of smaller size compared to primary neutrophils.https://doi.org/10.1038/s41598-020-78854-6 |
| spellingShingle | Kehinde Adebayo Babatunde Xiao Wang Alex Hopke Nils Lannes Pierre-Yves Mantel Daniel Irimia Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells Scientific Reports |
| title | Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells |
| title_full | Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells |
| title_fullStr | Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells |
| title_full_unstemmed | Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells |
| title_short | Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells |
| title_sort | chemotaxis and swarming in differentiated hl 60 neutrophil like cells |
| url | https://doi.org/10.1038/s41598-020-78854-6 |
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