DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL
In the present study, a rapid, sensitive, and selective method for determination of several synthetic analogues of gonadotropin-releasing hormone in human urine by solid-phase extraction and ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was developed. Variou...
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2017-04-01
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| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/85 |
| _version_ | 1826565743274098688 |
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| author | K. M. Shestakova G. A. Dudko M. A. Dikunets |
| author_facet | K. M. Shestakova G. A. Dudko M. A. Dikunets |
| author_sort | K. M. Shestakova |
| collection | DOAJ |
| description | In the present study, a rapid, sensitive, and selective method for determination of several synthetic analogues of gonadotropin-releasing hormone in human urine by solid-phase extraction and ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was developed. Various parameters affecting sample preparation, LC separation, and MS/MS detection were investigated, and optimized conditions were identified. The UPLC-MS/MS system was equipped with an electrospray ion source operating in positive ion mode with selected reaction monitoring. Leuprolide-13С6 was used as internal standard for analytes quantitative assessment. The proposed method was validated considering the parameters specificity, linearity (0.1-10 ng/ml), recovery (52-98%), limit of detection (0.1 ng/ml), matrix effects and stability. |
| first_indexed | 2024-04-10T03:29:03Z |
| format | Article |
| id | doaj.art-cbac293131154cc9bd9ddfa1cbd17b76 |
| institution | Directory Open Access Journal |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| last_indexed | 2025-03-14T10:40:36Z |
| publishDate | 2017-04-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj.art-cbac293131154cc9bd9ddfa1cbd17b762025-03-02T10:57:34ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752017-04-01122819910.32362/2410-6593-2017-12-2-81-9985DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROLK. M. Shestakova0G. A. Dudko1M. A. Dikunets2Federal State Budgetary Institution “Antidoping Centre”Federal State Budgetary Institution “Antidoping Centre”Federal State Budgetary Institution “Antidoping Centre”In the present study, a rapid, sensitive, and selective method for determination of several synthetic analogues of gonadotropin-releasing hormone in human urine by solid-phase extraction and ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was developed. Various parameters affecting sample preparation, LC separation, and MS/MS detection were investigated, and optimized conditions were identified. The UPLC-MS/MS system was equipped with an electrospray ion source operating in positive ion mode with selected reaction monitoring. Leuprolide-13С6 was used as internal standard for analytes quantitative assessment. The proposed method was validated considering the parameters specificity, linearity (0.1-10 ng/ml), recovery (52-98%), limit of detection (0.1 ng/ml), matrix effects and stability.https://www.finechem-mirea.ru/jour/article/view/85gonadotropin-releasing hormonegonadotropin-releasing hormone agonistssolidphase egonadotropin-releasing hormongonadotropin-releasing hormon agonistssolid-phase extractionultra-performance liquid chromatography coupled to tandem mass spectrometryuplcms/msultra-performance liquid chromatography coupled to high resolution mass spectrometryuplc-hrmsdoping control |
| spellingShingle | K. M. Shestakova G. A. Dudko M. A. Dikunets DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL Тонкие химические технологии gonadotropin-releasing hormone gonadotropin-releasing hormone agonists solidphase egonadotropin-releasing hormon gonadotropin-releasing hormon agonists solid-phase extraction ultra-performance liquid chromatography coupled to tandem mass spectrometry uplcms/ms ultra-performance liquid chromatography coupled to high resolution mass spectrometry uplc-hrms doping control |
| title | DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL |
| title_full | DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL |
| title_fullStr | DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL |
| title_full_unstemmed | DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL |
| title_short | DEVELOPMENT OF A TECHNIQUE FOR THE MASS-SPECTROMETRIC DETERMINATION OF GONADOTROPIN-RELEASING HORMONE AND ITS ANALOGS FOR DOPING CONTROL |
| title_sort | development of a technique for the mass spectrometric determination of gonadotropin releasing hormone and its analogs for doping control |
| topic | gonadotropin-releasing hormone gonadotropin-releasing hormone agonists solidphase egonadotropin-releasing hormon gonadotropin-releasing hormon agonists solid-phase extraction ultra-performance liquid chromatography coupled to tandem mass spectrometry uplcms/ms ultra-performance liquid chromatography coupled to high resolution mass spectrometry uplc-hrms doping control |
| url | https://www.finechem-mirea.ru/jour/article/view/85 |
| work_keys_str_mv | AT kmshestakova developmentofatechniqueforthemassspectrometricdeterminationofgonadotropinreleasinghormoneanditsanalogsfordopingcontrol AT gadudko developmentofatechniqueforthemassspectrometricdeterminationofgonadotropinreleasinghormoneanditsanalogsfordopingcontrol AT madikunets developmentofatechniqueforthemassspectrometricdeterminationofgonadotropinreleasinghormoneanditsanalogsfordopingcontrol |