<i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity
Lactic acid bacteria (LAB) have gained particular attention among different exopolysaccharide-producing microorganisms due to their safety status and effects on human health and food production. Exopolysaccharide-producing LAB play a crucial role in different ways, such as improving texture, mouthfe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/5/703 |
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| author | Shadi Pakroo Armin Tarrah Rohit Takur Manyu Wu Viviana Corich Alessio Giacomini |
| author_facet | Shadi Pakroo Armin Tarrah Rohit Takur Manyu Wu Viviana Corich Alessio Giacomini |
| author_sort | Shadi Pakroo |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) have gained particular attention among different exopolysaccharide-producing microorganisms due to their safety status and effects on human health and food production. Exopolysaccharide-producing LAB play a crucial role in different ways, such as improving texture, mouthfeel, controlling viscosity, and for low-calorie food production. In this study, we isolated a multifunctional strain with good exopolysaccharide production properties. <i>Limosilactobacillus fermentum</i> ING8 was isolated from an Indian traditional fermented milk (Dahi) and evaluated for its safety, enzymatic activity, NaCl resistance and temperature tolerance, milk coagulation, and storage stability. Finally, the complete genome of this strain was sequenced and subjected to safety in silico evaluation and genomic analysis. The results revealed that <i>L. fermentum</i> ING8 possesses relevant technological properties, such as exopolysaccharide production, antimicrobial activity, and galactose utilization. Besides, this strain showed very high stability to storage conditions at refrigeration temperature. In addition, the genomic analysis did not evidence any possible deleterious elements, such as acquired antibiotic resistance genes, virulence genes, or hemolysis-related genes. However, all structural genes related to the galactose operon and EPS production were detected. Therefore, <i>L. fermentum</i> ING8 can be considered a promising multifunctional bacterium to be proposed as non-starter in different types of dairy productions. |
| first_indexed | 2024-03-09T20:40:15Z |
| format | Article |
| id | doaj.art-cbad7658c2a64cc1983d5c8b03231596 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T20:40:15Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-cbad7658c2a64cc1983d5c8b032315962023-11-23T23:01:12ZengMDPI AGFoods2304-81582022-02-0111570310.3390/foods11050703<i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial ActivityShadi Pakroo0Armin Tarrah1Rohit Takur2Manyu Wu3Viviana Corich4Alessio Giacomini5Department of Agronomy Food Natural Resources Animal and Environment (DAFNAE), University of Padova, Viale dell’Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy Food Natural Resources Animal and Environment (DAFNAE), University of Padova, Viale dell’Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy Food Natural Resources Animal and Environment (DAFNAE), University of Padova, Viale dell’Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy Food Natural Resources Animal and Environment (DAFNAE), University of Padova, Viale dell’Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy Food Natural Resources Animal and Environment (DAFNAE), University of Padova, Viale dell’Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy Food Natural Resources Animal and Environment (DAFNAE), University of Padova, Viale dell’Università 16, Legnaro, 35020 Padova, ItalyLactic acid bacteria (LAB) have gained particular attention among different exopolysaccharide-producing microorganisms due to their safety status and effects on human health and food production. Exopolysaccharide-producing LAB play a crucial role in different ways, such as improving texture, mouthfeel, controlling viscosity, and for low-calorie food production. In this study, we isolated a multifunctional strain with good exopolysaccharide production properties. <i>Limosilactobacillus fermentum</i> ING8 was isolated from an Indian traditional fermented milk (Dahi) and evaluated for its safety, enzymatic activity, NaCl resistance and temperature tolerance, milk coagulation, and storage stability. Finally, the complete genome of this strain was sequenced and subjected to safety in silico evaluation and genomic analysis. The results revealed that <i>L. fermentum</i> ING8 possesses relevant technological properties, such as exopolysaccharide production, antimicrobial activity, and galactose utilization. Besides, this strain showed very high stability to storage conditions at refrigeration temperature. In addition, the genomic analysis did not evidence any possible deleterious elements, such as acquired antibiotic resistance genes, virulence genes, or hemolysis-related genes. However, all structural genes related to the galactose operon and EPS production were detected. Therefore, <i>L. fermentum</i> ING8 can be considered a promising multifunctional bacterium to be proposed as non-starter in different types of dairy productions.https://www.mdpi.com/2304-8158/11/5/703exopolysaccharidesgalactosedahi<i>Limosilactobacillus fermentum</i> |
| spellingShingle | Shadi Pakroo Armin Tarrah Rohit Takur Manyu Wu Viviana Corich Alessio Giacomini <i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity Foods exopolysaccharides galactose dahi <i>Limosilactobacillus fermentum</i> |
| title | <i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity |
| title_full | <i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity |
| title_fullStr | <i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity |
| title_full_unstemmed | <i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity |
| title_short | <i>Limosilactobacillus fermentum</i> ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity |
| title_sort | i limosilactobacillus fermentum i ing8 a potential multifunctional non starter strain with relevant technological properties and antimicrobial activity |
| topic | exopolysaccharides galactose dahi <i>Limosilactobacillus fermentum</i> |
| url | https://www.mdpi.com/2304-8158/11/5/703 |
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