Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers
Abstract Background The objectives of this study were to reveal the anthocyanin biosynthesis metabolic pathway in white and purple flowers of Salvia miltiorrhiza using metabolomics and transcriptomics, to identify different anthocyanin metabolites, and to analyze the differentially expressed genes i...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-07-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02553-7 |
| _version_ | 1828848545704181760 |
|---|---|
| author | Tao Jiang Meidi Zhang Chunxiu Wen Xiaoliang Xie Wei Tian Saiqun Wen Ruike Lu Lingdi Liu |
| author_facet | Tao Jiang Meidi Zhang Chunxiu Wen Xiaoliang Xie Wei Tian Saiqun Wen Ruike Lu Lingdi Liu |
| author_sort | Tao Jiang |
| collection | DOAJ |
| description | Abstract Background The objectives of this study were to reveal the anthocyanin biosynthesis metabolic pathway in white and purple flowers of Salvia miltiorrhiza using metabolomics and transcriptomics, to identify different anthocyanin metabolites, and to analyze the differentially expressed genes involved in anthocyanin biosynthesis. Results We analyzed the metabolomics and transcriptomics data of S. miltiorrhiza flowers. A total of 1994 differentially expressed genes and 84 flavonoid metabolites were identified between the white and purple flowers of S. miltiorrhiza. Integrated analysis of transcriptomics and metabolomics showed that cyanidin 3,5-O-diglucoside, malvidin 3,5-diglucoside, and cyanidin 3-O-galactoside were mainly responsible for the purple flower color of S. miltiorrhiza. A total of 100 unigenes encoding 10 enzymes were identified as candidate genes involved in anthocyanin biosynthesis in S. miltiorrhiza flowers. Low expression of the ANS gene decreased the anthocyanin content but enhanced the accumulation of flavonoids in S. miltiorrhiza flowers. Conclusions Our results provide valuable information on the anthocyanin metabolites and the candidate genes involved in the anthocyanin biosynthesis pathways in S. miltiorrhiza. |
| first_indexed | 2024-12-12T22:32:15Z |
| format | Article |
| id | doaj.art-cbb09378554d4fd9b7cb610059b633ab |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T22:32:15Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-cbb09378554d4fd9b7cb610059b633ab2022-12-22T00:09:34ZengBMCBMC Plant Biology1471-22292020-07-0120111310.1186/s12870-020-02553-7Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowersTao Jiang0Meidi Zhang1Chunxiu Wen2Xiaoliang Xie3Wei Tian4Saiqun Wen5Ruike Lu6Lingdi Liu7Institute of Cash Crops, Hebei Academy of Agricultural and Forestry SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Cash Crops, Hebei Academy of Agricultural and Forestry SciencesInstitute of Cash Crops, Hebei Academy of Agricultural and Forestry SciencesInstitute of Cash Crops, Hebei Academy of Agricultural and Forestry SciencesInstitute of Cash Crops, Hebei Academy of Agricultural and Forestry SciencesInstitute of Cash Crops, Hebei Academy of Agricultural and Forestry SciencesInstitute of Cash Crops, Hebei Academy of Agricultural and Forestry SciencesAbstract Background The objectives of this study were to reveal the anthocyanin biosynthesis metabolic pathway in white and purple flowers of Salvia miltiorrhiza using metabolomics and transcriptomics, to identify different anthocyanin metabolites, and to analyze the differentially expressed genes involved in anthocyanin biosynthesis. Results We analyzed the metabolomics and transcriptomics data of S. miltiorrhiza flowers. A total of 1994 differentially expressed genes and 84 flavonoid metabolites were identified between the white and purple flowers of S. miltiorrhiza. Integrated analysis of transcriptomics and metabolomics showed that cyanidin 3,5-O-diglucoside, malvidin 3,5-diglucoside, and cyanidin 3-O-galactoside were mainly responsible for the purple flower color of S. miltiorrhiza. A total of 100 unigenes encoding 10 enzymes were identified as candidate genes involved in anthocyanin biosynthesis in S. miltiorrhiza flowers. Low expression of the ANS gene decreased the anthocyanin content but enhanced the accumulation of flavonoids in S. miltiorrhiza flowers. Conclusions Our results provide valuable information on the anthocyanin metabolites and the candidate genes involved in the anthocyanin biosynthesis pathways in S. miltiorrhiza.http://link.springer.com/article/10.1186/s12870-020-02553-7Medicinal plantAnthocyaninTranscriptomeMetaboliteS. Miltiorrhiza |
| spellingShingle | Tao Jiang Meidi Zhang Chunxiu Wen Xiaoliang Xie Wei Tian Saiqun Wen Ruike Lu Lingdi Liu Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers BMC Plant Biology Medicinal plant Anthocyanin Transcriptome Metabolite S. Miltiorrhiza |
| title | Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers |
| title_full | Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers |
| title_fullStr | Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers |
| title_full_unstemmed | Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers |
| title_short | Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers |
| title_sort | integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in salvia miltiorrhiza bge flowers |
| topic | Medicinal plant Anthocyanin Transcriptome Metabolite S. Miltiorrhiza |
| url | http://link.springer.com/article/10.1186/s12870-020-02553-7 |
| work_keys_str_mv | AT taojiang integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers AT meidizhang integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers AT chunxiuwen integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers AT xiaoliangxie integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers AT weitian integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers AT saiqunwen integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers AT ruikelu integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers AT lingdiliu integratedmetabolomicandtranscriptomicanalysisoftheanthocyaninregulatorynetworksinsalviamiltiorrhizabgeflowers |