Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt.
<h4>Background</h4>This is the first Egyptian nationwide study for derivation of reference intervals (RIs) for 34 major chemistry analytes. It was conducted as a part of the global initiative by the IFCC Committee on Reference Intervals and Decision Limits (C-RIDL) for establishing count...
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| Format: | Article |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0236772 |
| _version_ | 1818743202945957888 |
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| author | Heba Baz Kiyoshi Ichihara May Selim Ahmed Awad Sarah Aglan Dalia Ramadan Amina Hassab Lamia Mansour Ola Elgaddar |
| author_facet | Heba Baz Kiyoshi Ichihara May Selim Ahmed Awad Sarah Aglan Dalia Ramadan Amina Hassab Lamia Mansour Ola Elgaddar |
| author_sort | Heba Baz |
| collection | DOAJ |
| description | <h4>Background</h4>This is the first Egyptian nationwide study for derivation of reference intervals (RIs) for 34 major chemistry analytes. It was conducted as a part of the global initiative by the IFCC Committee on Reference Intervals and Decision Limits (C-RIDL) for establishing country-specific RIs based on a harmonized protocol.<h4>Methods</h4>691 apparently healthy volunteers aged ≥18 years were recruited from multiple regions in Egypt. Serum specimens were analyzed in two centers. The harmonization and standardization of test results were achieved by measuring value-assigned serum panel provided by C-RIDL. The RIs were calculated by parametric method. Sources of variation of reference values (RVs) were evaluated by multiple regression analysis. The need for partitioning by sex, age, and region was judged primarily by standard deviation ratio (SDR).<h4>Results</h4>Gender-specific RIs were required for six analytes including total bilirubin (TBil), aspartate and alanine aminotransferase (AST, ALT). Seven analytes required age-partitioning including glucose and low-density lipoprotein cholesterol (LDL-C). Regional differences were observed between northern and southern Egypt for direct bilirubin, glucose, and high-density-lipoprotein cholesterol (HDL-C) with all their RVs lower in southern Egypt. Compared with other collaborating countries, the features of Egyptian RVs were lower HDL-C and TBil and higher TG and C-reactive protein. In addition, BMI showed weak association with most of nutritional markers. These features were shared with two other Middle Eastern countries: Saudi Arabia and Turkey.<h4>Conclusion</h4>The standardized RIs established by this study can be used as common Egyptian RI, except for a few analytes that showed regional differences. Despite high prevalence of obesity among Egyptians, their RVs of nutritional markers are less sensitive to increased BMI, compared to other collaborating countries. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T02:24:40Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-cbb18e831f8d443291de199a6cff449c2022-12-21T21:24:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01163e023677210.1371/journal.pone.0236772Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt.Heba BazKiyoshi IchiharaMay SelimAhmed AwadSarah AglanDalia RamadanAmina HassabLamia MansourOla Elgaddar<h4>Background</h4>This is the first Egyptian nationwide study for derivation of reference intervals (RIs) for 34 major chemistry analytes. It was conducted as a part of the global initiative by the IFCC Committee on Reference Intervals and Decision Limits (C-RIDL) for establishing country-specific RIs based on a harmonized protocol.<h4>Methods</h4>691 apparently healthy volunteers aged ≥18 years were recruited from multiple regions in Egypt. Serum specimens were analyzed in two centers. The harmonization and standardization of test results were achieved by measuring value-assigned serum panel provided by C-RIDL. The RIs were calculated by parametric method. Sources of variation of reference values (RVs) were evaluated by multiple regression analysis. The need for partitioning by sex, age, and region was judged primarily by standard deviation ratio (SDR).<h4>Results</h4>Gender-specific RIs were required for six analytes including total bilirubin (TBil), aspartate and alanine aminotransferase (AST, ALT). Seven analytes required age-partitioning including glucose and low-density lipoprotein cholesterol (LDL-C). Regional differences were observed between northern and southern Egypt for direct bilirubin, glucose, and high-density-lipoprotein cholesterol (HDL-C) with all their RVs lower in southern Egypt. Compared with other collaborating countries, the features of Egyptian RVs were lower HDL-C and TBil and higher TG and C-reactive protein. In addition, BMI showed weak association with most of nutritional markers. These features were shared with two other Middle Eastern countries: Saudi Arabia and Turkey.<h4>Conclusion</h4>The standardized RIs established by this study can be used as common Egyptian RI, except for a few analytes that showed regional differences. Despite high prevalence of obesity among Egyptians, their RVs of nutritional markers are less sensitive to increased BMI, compared to other collaborating countries.https://doi.org/10.1371/journal.pone.0236772 |
| spellingShingle | Heba Baz Kiyoshi Ichihara May Selim Ahmed Awad Sarah Aglan Dalia Ramadan Amina Hassab Lamia Mansour Ola Elgaddar Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt. PLoS ONE |
| title | Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt. |
| title_full | Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt. |
| title_fullStr | Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt. |
| title_full_unstemmed | Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt. |
| title_short | Establishment of reference intervals of clinical chemistry analytes for the adult population in Egypt. |
| title_sort | establishment of reference intervals of clinical chemistry analytes for the adult population in egypt |
| url | https://doi.org/10.1371/journal.pone.0236772 |
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