Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditions
Strawberry (Fragaria ananassa) plants showing symptoms of leaf spot were found in the producing areas of Manchay Bajo, Pachacamac District, Lima, Peru. The responsible pathogen was isolated and observed under a microscope to examine its morphological characteristics and the size of its structures, r...
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| Format: | Article |
| Language: | English |
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Universidad Nacional Agraria La Molina
2019-08-01
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| Series: | Peruvian Journal of Agronomy |
| Subjects: | |
| Online Access: | https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1315 |
| _version_ | 1818838124449497088 |
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| author | C. Orellana L. Mattos |
| author_facet | C. Orellana L. Mattos |
| author_sort | C. Orellana |
| collection | DOAJ |
| description | Strawberry (Fragaria ananassa) plants showing symptoms of leaf spot were found in the producing areas of Manchay Bajo, Pachacamac District, Lima, Peru. The responsible pathogen was isolated and observed under a microscope to examine its morphological characteristics and the size of its structures, resulting in its identification as the fungus Corynespora cassiicola. Healthy strawberry plants that had been inoculated with C. cassiicola in a greenhouse developed lesions after 3 days and exhibited symptoms consisting of dark brown spots that had light brown centers and were surrounded by yellow halos, which coalesced and subsequently caused necrosis of the whole leaf. In vitro tests demonstrated that the fungus Trichoderma harzianum strain T-22 showed antagonistic activity against C. cassiicola, causing 41.8% mycelial growth inhibition, and the fungicides fosetyl-aluminum (Aliette®) and thiabendazole (Mertect®a) inhibited mycelial growth by 100.00% and 96.32%, respectively. However, in vivo, 0.75 g/L of the fungicide azoxystrobim (Stronsil®) gave the best control of C. cassiicola and the lowest area under the disease progress curve (AUDPC), followed by 1.5 mL/L of thiabendazole (Mertect) under greenhouse conditions. The isolate was found to be pathogenic, and its morphological identification was confirmed. |
| first_indexed | 2024-12-19T03:33:24Z |
| format | Article |
| id | doaj.art-cbb51b84a8d24c758313e3fd9301a547 |
| institution | Directory Open Access Journal |
| issn | 2616-4477 |
| language | English |
| last_indexed | 2024-12-19T03:33:24Z |
| publishDate | 2019-08-01 |
| publisher | Universidad Nacional Agraria La Molina |
| record_format | Article |
| series | Peruvian Journal of Agronomy |
| spelling | doaj.art-cbb51b84a8d24c758313e3fd9301a5472022-12-21T20:37:27ZengUniversidad Nacional Agraria La MolinaPeruvian Journal of Agronomy2616-44772019-08-0132576710.21704/pja.v3i2.13151242Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditionsC. Orellana0L. Mattos1Mg. Sc. Phytopathology, of the Universidad Nacional Agraria La Molina.Senior Professor of the Dept. of Phytopathology, Faculty of Agronomy of the Universidad Nacional Agraria La MolinaStrawberry (Fragaria ananassa) plants showing symptoms of leaf spot were found in the producing areas of Manchay Bajo, Pachacamac District, Lima, Peru. The responsible pathogen was isolated and observed under a microscope to examine its morphological characteristics and the size of its structures, resulting in its identification as the fungus Corynespora cassiicola. Healthy strawberry plants that had been inoculated with C. cassiicola in a greenhouse developed lesions after 3 days and exhibited symptoms consisting of dark brown spots that had light brown centers and were surrounded by yellow halos, which coalesced and subsequently caused necrosis of the whole leaf. In vitro tests demonstrated that the fungus Trichoderma harzianum strain T-22 showed antagonistic activity against C. cassiicola, causing 41.8% mycelial growth inhibition, and the fungicides fosetyl-aluminum (Aliette®) and thiabendazole (Mertect®a) inhibited mycelial growth by 100.00% and 96.32%, respectively. However, in vivo, 0.75 g/L of the fungicide azoxystrobim (Stronsil®) gave the best control of C. cassiicola and the lowest area under the disease progress curve (AUDPC), followed by 1.5 mL/L of thiabendazole (Mertect) under greenhouse conditions. The isolate was found to be pathogenic, and its morphological identification was confirmed.https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1315leaf spotcorynespora cassiicolastrawberryfragaria ananassa |
| spellingShingle | C. Orellana L. Mattos Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditions Peruvian Journal of Agronomy leaf spot corynespora cassiicola strawberry fragaria ananassa |
| title | Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditions |
| title_full | Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditions |
| title_fullStr | Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditions |
| title_full_unstemmed | Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditions |
| title_short | Characterization and control of the causal agent of leaf spot in strawberry (Fragaria ananassa) under greenhouse conditions |
| title_sort | characterization and control of the causal agent of leaf spot in strawberry fragaria ananassa under greenhouse conditions |
| topic | leaf spot corynespora cassiicola strawberry fragaria ananassa |
| url | https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1315 |
| work_keys_str_mv | AT corellana characterizationandcontrolofthecausalagentofleafspotinstrawberryfragariaananassaundergreenhouseconditions AT lmattos characterizationandcontrolofthecausalagentofleafspotinstrawberryfragariaananassaundergreenhouseconditions |