Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid
Abstract Background Equine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona remains an antemortem diagnostic challenge in some horses. Recent work suggested the use of real‐time PCR (rtPCR) on cerebrospinal fluid (CSF) as a promising diagnostic tool. Objective To evaluate the sensitiv...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.16826 |
| _version_ | 1797729059163078656 |
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| author | Carla Katherine Enriquez Jennifer K. Morrow Amy Graves Amy Johnson |
| author_facet | Carla Katherine Enriquez Jennifer K. Morrow Amy Graves Amy Johnson |
| author_sort | Carla Katherine Enriquez |
| collection | DOAJ |
| description | Abstract Background Equine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona remains an antemortem diagnostic challenge in some horses. Recent work suggested the use of real‐time PCR (rtPCR) on cerebrospinal fluid (CSF) as a promising diagnostic tool. Objective To evaluate the sensitivity and specificity of S. neurona rtPCR on CSF for EPM diagnosis using horses with EPM and S. neurona‐seropositive horses with other neurologic conditions. Animals Ninety‐nine horses with neurologic disease that underwent complete neurologic examination, CSF collection, and, if euthanized, necropsy including the central nervous system (CNS). Methods Retrospective case‐control study using banked CSF samples. Samples from horses with neurologic abnormalities and necropsy‐confirmed EPM diagnosis, presumptive EPM diagnosis using strict criteria (SnSAG2/4/3 ELISA serum:CSF titer ratios <50) and horses diagnosed with other neurologic diseases were used. Results Fifty‐two horses had EPM; 23 were confirmed on necropsy, and 29 were presumptive clinical diagnoses. The other 47 horses all had necropsy‐confirmed diagnoses. Four of the 47 horses had normal neurologic findings on necropsy and the remaining 43 horses had neurologic diseases including equine degenerative myeloencephalopathy (EDM), cervical vertebral stenotic myelopathy, trauma, and other miscellaneous conditions. One CSF sample was weakly positive for S. neurona by rtPCR, this sample was obtained from a horse with confirmed EDM. Samples from the other 98 horses were negative for S. neurona by rtPCR. Conclusions and Clinical Importance Our study contradicts previous conclusions that S. neurona rtPCR is potentially useful for EPM diagnosis, because our results indicate that the assay has a low sensitivity (0%) for EPM. |
| first_indexed | 2024-03-12T11:23:28Z |
| format | Article |
| id | doaj.art-cbb709fa318b4f11b965f525c02feb92 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-03-12T11:23:28Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-cbb709fa318b4f11b965f525c02feb922023-09-01T14:18:40ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762023-09-013751893189810.1111/jvim.16826Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluidCarla Katherine Enriquez0Jennifer K. Morrow1Amy Graves2Amy Johnson3Department of Clinical Studies University of Pennsylvania New Bolton Center Hospital for Large Animals Kennett Square Pennsylvania USAEquine Diagnostic Solutions, LLC Lexington Kentucky USAEquine Diagnostic Solutions, LLC Lexington Kentucky USADepartment of Clinical Studies University of Pennsylvania New Bolton Center Hospital for Large Animals Kennett Square Pennsylvania USAAbstract Background Equine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona remains an antemortem diagnostic challenge in some horses. Recent work suggested the use of real‐time PCR (rtPCR) on cerebrospinal fluid (CSF) as a promising diagnostic tool. Objective To evaluate the sensitivity and specificity of S. neurona rtPCR on CSF for EPM diagnosis using horses with EPM and S. neurona‐seropositive horses with other neurologic conditions. Animals Ninety‐nine horses with neurologic disease that underwent complete neurologic examination, CSF collection, and, if euthanized, necropsy including the central nervous system (CNS). Methods Retrospective case‐control study using banked CSF samples. Samples from horses with neurologic abnormalities and necropsy‐confirmed EPM diagnosis, presumptive EPM diagnosis using strict criteria (SnSAG2/4/3 ELISA serum:CSF titer ratios <50) and horses diagnosed with other neurologic diseases were used. Results Fifty‐two horses had EPM; 23 were confirmed on necropsy, and 29 were presumptive clinical diagnoses. The other 47 horses all had necropsy‐confirmed diagnoses. Four of the 47 horses had normal neurologic findings on necropsy and the remaining 43 horses had neurologic diseases including equine degenerative myeloencephalopathy (EDM), cervical vertebral stenotic myelopathy, trauma, and other miscellaneous conditions. One CSF sample was weakly positive for S. neurona by rtPCR, this sample was obtained from a horse with confirmed EDM. Samples from the other 98 horses were negative for S. neurona by rtPCR. Conclusions and Clinical Importance Our study contradicts previous conclusions that S. neurona rtPCR is potentially useful for EPM diagnosis, because our results indicate that the assay has a low sensitivity (0%) for EPM.https://doi.org/10.1111/jvim.16826CSF analysisequine protozoal myeloencephalopathyhorsemolecular biologyneurologyparasitology |
| spellingShingle | Carla Katherine Enriquez Jennifer K. Morrow Amy Graves Amy Johnson Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid Journal of Veterinary Internal Medicine CSF analysis equine protozoal myeloencephalopathy horse molecular biology neurology parasitology |
| title | Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid |
| title_full | Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid |
| title_fullStr | Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid |
| title_full_unstemmed | Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid |
| title_short | Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid |
| title_sort | evaluation of real time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid |
| topic | CSF analysis equine protozoal myeloencephalopathy horse molecular biology neurology parasitology |
| url | https://doi.org/10.1111/jvim.16826 |
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