Fibrin gel as a scaffold for skin substitute – production and clinical experience
The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaffolds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sestre Milosrdnice University hospital, Institute of Clinical Medical Research
2016-01-01
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| Series: | Acta Clinica Croatica |
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| Online Access: | https://hrcak.srce.hr/file/243094 |
| _version_ | 1797207972575707136 |
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| author | Antun Kljenak Mirna Tominac Trcin Marina Bujić Tamara Dolenec Martina Jevak Gordan Mršić Gordana Zmiš Zoran Barčot Ante Muljačić Maja Popović |
| author_facet | Antun Kljenak Mirna Tominac Trcin Marina Bujić Tamara Dolenec Martina Jevak Gordan Mršić Gordana Zmiš Zoran Barčot Ante Muljačić Maja Popović |
| author_sort | Antun Kljenak |
| collection | DOAJ |
| description | The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaffolds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in fibrin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fluorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed. After hydrosurgical preparation of deep burn necrotic tissue, wound bed was prepared for skin substitutes. Progress of healing was documented using visual estimation and photos. Scanning electron microscope images showed good cell attachment and colony spreading of keratinocytes and fibroblasts on fibrin scaffold. Immunofluorescent staining of cell cultures on fibrin scaffold showed expression of vimentin, a marker of fibroblast cells, cytokeratin 19, a marker of epithelial stem cells, as well as involucrin, a marker of differentiated keratinocytes. Clinical results clearly showed that appearance of the skin did not differ significantly from the areas of transplanted skin using split-thickness skin graft techniques. In conclusion, using these fibrin-cultured autografts on massive full-thickness burn resulted in good healing. |
| first_indexed | 2024-04-24T09:31:24Z |
| format | Article |
| id | doaj.art-cbb94ebe1fe4479d91848dd9cd72b16e |
| institution | Directory Open Access Journal |
| issn | 0353-9466 1333-9451 |
| language | English |
| last_indexed | 2024-04-24T09:31:24Z |
| publishDate | 2016-01-01 |
| publisher | Sestre Milosrdnice University hospital, Institute of Clinical Medical Research |
| record_format | Article |
| series | Acta Clinica Croatica |
| spelling | doaj.art-cbb94ebe1fe4479d91848dd9cd72b16e2024-04-15T13:55:33ZengSestre Milosrdnice University hospital, Institute of Clinical Medical ResearchActa Clinica Croatica0353-94661333-94512016-01-0155.2.27928810.20471/acc.2016.55.02.15Fibrin gel as a scaffold for skin substitute – production and clinical experienceAntun Kljenak0Mirna Tominac Trcin1Marina Bujić2Tamara Dolenec3Martina Jevak4Gordan Mršić5Gordana Zmiš6Zoran Barčot7Ante Muljačić8Maja Popović9Zagreb Children’s Hospital, Zagreb, CroatiaTissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaTissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaTissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaTissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaIvan Vučetić Center for Forensic Investigations, Research and Expertise, Zagreb, Croatia; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaTissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaZagreb Children’s Hospital, Zagreb, CroatiaTissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaDepartment of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaThe purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaffolds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in fibrin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fluorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed. After hydrosurgical preparation of deep burn necrotic tissue, wound bed was prepared for skin substitutes. Progress of healing was documented using visual estimation and photos. Scanning electron microscope images showed good cell attachment and colony spreading of keratinocytes and fibroblasts on fibrin scaffold. Immunofluorescent staining of cell cultures on fibrin scaffold showed expression of vimentin, a marker of fibroblast cells, cytokeratin 19, a marker of epithelial stem cells, as well as involucrin, a marker of differentiated keratinocytes. Clinical results clearly showed that appearance of the skin did not differ significantly from the areas of transplanted skin using split-thickness skin graft techniques. In conclusion, using these fibrin-cultured autografts on massive full-thickness burn resulted in good healing.https://hrcak.srce.hr/file/243094Cells, culturedFibrinSkin, artificialBurns |
| spellingShingle | Antun Kljenak Mirna Tominac Trcin Marina Bujić Tamara Dolenec Martina Jevak Gordan Mršić Gordana Zmiš Zoran Barčot Ante Muljačić Maja Popović Fibrin gel as a scaffold for skin substitute – production and clinical experience Acta Clinica Croatica Cells, cultured Fibrin Skin, artificial Burns |
| title | Fibrin gel as a scaffold for skin substitute – production and clinical experience |
| title_full | Fibrin gel as a scaffold for skin substitute – production and clinical experience |
| title_fullStr | Fibrin gel as a scaffold for skin substitute – production and clinical experience |
| title_full_unstemmed | Fibrin gel as a scaffold for skin substitute – production and clinical experience |
| title_short | Fibrin gel as a scaffold for skin substitute – production and clinical experience |
| title_sort | fibrin gel as a scaffold for skin substitute production and clinical experience |
| topic | Cells, cultured Fibrin Skin, artificial Burns |
| url | https://hrcak.srce.hr/file/243094 |
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