Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cells
Zinc oxide nanoparticles (ZnO-NPs) were synthesized using ginger (Zingiber officinale) extracts in a green synthesis approach and evaluated their in vitro cytotoxicity effect on the MDA-MB 231 breast cancer cell line. The bottom-up approach was employed to develop the green-synthesized ginger-encaps...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=4;spage=325;epage=331;aulast=Al-Suwayyid |
| _version_ | 1797222540234457088 |
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| author | Lobna Shakir Abed Al-Suwayyid Ashok Kumar Janakiraman Sharmanee Thiagarajah Baskaran Gunasekaran Kushagra Khanna Abhishek Kumar Jamal Moideen Muthu Mohamed Ling Shing Wong |
| author_facet | Lobna Shakir Abed Al-Suwayyid Ashok Kumar Janakiraman Sharmanee Thiagarajah Baskaran Gunasekaran Kushagra Khanna Abhishek Kumar Jamal Moideen Muthu Mohamed Ling Shing Wong |
| author_sort | Lobna Shakir Abed Al-Suwayyid |
| collection | DOAJ |
| description | Zinc oxide nanoparticles (ZnO-NPs) were synthesized using ginger (Zingiber officinale) extracts in a green synthesis approach and evaluated their in vitro cytotoxicity effect on the MDA-MB 231 breast cancer cell line. The bottom-up approach was employed to develop the green-synthesized ginger-encapsulated ZnO-NPs (GZnO-NPs) without using hazardous substances. The most substantial Fourier-transform infrared absorption peak of the ginger root extract was seen at 1634.24 cm−1. The peak also confirmed the presence of ginger root extract-encapsulated ZnO-NPs at 1556.79, 1471.54, and 1019.83 cm−1. It indicates that the biomolecules found in plant extracts behave as capping agents, aiding in the formation of nanoparticles. The mean particle sizes (PSs) of optimized GZnO-NPs of the ratios 1:2 were found to be 104.01 ± 7.12 nm with a zeta potential of −11.5 ± 1.31 mV. The X-ray diffraction and scanning electron microscope analysis confirmed that the prepared nanoparticles were spherical and crystalline, with PS ranging from 100 to 150 nm. The GZnO-NPs were subjected to MTT assay and cellular migration potential, and it was found that the inhibitory concentration on the MDA-MB 231 (breast) cancer cell line and scratch area showed a dose-dependent efficacy. The successfully green-synthesized GZnO-NPs effectively induced cell death in the MDA-MB 231 cancer cell line. The scratch assay results confirmed that prepared GZnO-NPs inhibited the proliferation and migration of cancerous cells. |
| first_indexed | 2024-04-24T13:22:57Z |
| format | Article |
| id | doaj.art-cbbd7205ab8e48698a6687616140eb4b |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-04-24T13:22:57Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-cbbd7205ab8e48698a6687616140eb4b2024-04-04T11:28:38ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942023-01-0114432533110.4103/JAPTR.JAPTR_313_23Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cellsLobna Shakir Abed Al-SuwayyidAshok Kumar JanakiramanSharmanee ThiagarajahBaskaran GunasekaranKushagra KhannaAbhishek KumarJamal Moideen Muthu MohamedLing Shing WongZinc oxide nanoparticles (ZnO-NPs) were synthesized using ginger (Zingiber officinale) extracts in a green synthesis approach and evaluated their in vitro cytotoxicity effect on the MDA-MB 231 breast cancer cell line. The bottom-up approach was employed to develop the green-synthesized ginger-encapsulated ZnO-NPs (GZnO-NPs) without using hazardous substances. The most substantial Fourier-transform infrared absorption peak of the ginger root extract was seen at 1634.24 cm−1. The peak also confirmed the presence of ginger root extract-encapsulated ZnO-NPs at 1556.79, 1471.54, and 1019.83 cm−1. It indicates that the biomolecules found in plant extracts behave as capping agents, aiding in the formation of nanoparticles. The mean particle sizes (PSs) of optimized GZnO-NPs of the ratios 1:2 were found to be 104.01 ± 7.12 nm with a zeta potential of −11.5 ± 1.31 mV. The X-ray diffraction and scanning electron microscope analysis confirmed that the prepared nanoparticles were spherical and crystalline, with PS ranging from 100 to 150 nm. The GZnO-NPs were subjected to MTT assay and cellular migration potential, and it was found that the inhibitory concentration on the MDA-MB 231 (breast) cancer cell line and scratch area showed a dose-dependent efficacy. The successfully green-synthesized GZnO-NPs effectively induced cell death in the MDA-MB 231 cancer cell line. The scratch assay results confirmed that prepared GZnO-NPs inhibited the proliferation and migration of cancerous cells.http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=4;spage=325;epage=331;aulast=Al-Suwayyidcellular migration potentialginger root extractgreen synthesismtt assayzinc oxide nanoparticles |
| spellingShingle | Lobna Shakir Abed Al-Suwayyid Ashok Kumar Janakiraman Sharmanee Thiagarajah Baskaran Gunasekaran Kushagra Khanna Abhishek Kumar Jamal Moideen Muthu Mohamed Ling Shing Wong Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cells Journal of Advanced Pharmaceutical Technology & Research cellular migration potential ginger root extract green synthesis mtt assay zinc oxide nanoparticles |
| title | Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cells |
| title_full | Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cells |
| title_fullStr | Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cells |
| title_full_unstemmed | Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cells |
| title_short | Green synthesis of ginger-encapsulated zinc oxide nanoparticles: Unveiling their characterization and selective cytotoxicity on MDA-MB 231 breast cancer cells |
| title_sort | green synthesis of ginger encapsulated zinc oxide nanoparticles unveiling their characterization and selective cytotoxicity on mda mb 231 breast cancer cells |
| topic | cellular migration potential ginger root extract green synthesis mtt assay zinc oxide nanoparticles |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=4;spage=325;epage=331;aulast=Al-Suwayyid |
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