Probing the Dynamics of <i>Streptococcus pyogenes</i> Cas9 Endonuclease Bound to the sgRNA Complex Using Hydrogen-Deuterium Exchange Mass Spectrometry

Probing the Dynamics of <i>Streptococcus pyogenes</i> Cas9 Endonuclease Bound to the sgRNA Complex Using Hydrogen-Deuterium Exchange Mass Spectrometry

The Cas9 endonuclease is an essential component of the CRISPR–Cas-based genome editing tools. The attainment of high specificity and efficiency of Cas9 during targetted DNA cleavage is the main problem that limits the clinical application of the CRISPR–Cas9 system. A deep understanding of the Cas9 m...

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Bibliographic Details
Main Authors: Polina V. Zhdanova, Alexander A. Chernonosov, Daria V. Prokhorova, Grigory A. Stepanov, Lyubov Yu. Kanazhevskaya, Vladimir V. Koval
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:International Journal of Molecular Sciences
Subjects:
HDX-MS
hydrogen-deuterium exchange mass spectrometry
CRISPR–Cas systems
Cas9
single guide RNA
molecular dynamics
Online Access:https://www.mdpi.com/1422-0067/23/3/1129

Internet

https://www.mdpi.com/1422-0067/23/3/1129

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