Biofilm Eradication Using Biogenic Silver Nanoparticles
Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silve...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/9/2023 |
| _version_ | 1797569595829125120 |
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| author | María Belén Estevez Sofía Raffaelli Scott G. Mitchell Ricardo Faccio Silvana Alborés |
| author_facet | María Belén Estevez Sofía Raffaelli Scott G. Mitchell Ricardo Faccio Silvana Alborés |
| author_sort | María Belén Estevez |
| collection | DOAJ |
| description | Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of <i>Phanerochaete chrysosporium</i>, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45–nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to −30 mV and showed colloidal stability from pH 3–9 and under conditions of high ionic strength (<i>[NaCl]</i> = 10–500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-<i>E. coli</i> interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of <i>E. coli</i> cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of <i>E. coli</i> and <i>C. albicans</i> biofilms. |
| first_indexed | 2024-03-10T20:12:49Z |
| format | Article |
| id | doaj.art-cbc15f796fb84201a26a7c0d117f05b4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:12:49Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cbc15f796fb84201a26a7c0d117f05b42023-11-19T22:48:10ZengMDPI AGMolecules1420-30492020-04-01259202310.3390/molecules25092023Biofilm Eradication Using Biogenic Silver NanoparticlesMaría Belén Estevez0Sofía Raffaelli1Scott G. Mitchell2Ricardo Faccio3Silvana Alborés4Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, UruguayÁrea de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, UruguayInstituto de Ciencia de Materiales de Aragón (ICMA), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Zaragoza, 50009 Zaragoza, SpainCentro NanoMat & Grupo Física, Departamento de Experimentación y Teoría de la Estructura de la Materia y sus Aplicaciones (DETEMA), Facultad de Química, Universidad de la República, Montevideo 11800, UruguayÁrea de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, UruguayMicroorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of <i>Phanerochaete chrysosporium</i>, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45–nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to −30 mV and showed colloidal stability from pH 3–9 and under conditions of high ionic strength (<i>[NaCl]</i> = 10–500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-<i>E. coli</i> interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of <i>E. coli</i> cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of <i>E. coli</i> and <i>C. albicans</i> biofilms.https://www.mdpi.com/1420-3049/25/9/2023biogenic nanoparticlessilver nanoparticlesantimicrobialantibiofilm |
| spellingShingle | María Belén Estevez Sofía Raffaelli Scott G. Mitchell Ricardo Faccio Silvana Alborés Biofilm Eradication Using Biogenic Silver Nanoparticles Molecules biogenic nanoparticles silver nanoparticles antimicrobial antibiofilm |
| title | Biofilm Eradication Using Biogenic Silver Nanoparticles |
| title_full | Biofilm Eradication Using Biogenic Silver Nanoparticles |
| title_fullStr | Biofilm Eradication Using Biogenic Silver Nanoparticles |
| title_full_unstemmed | Biofilm Eradication Using Biogenic Silver Nanoparticles |
| title_short | Biofilm Eradication Using Biogenic Silver Nanoparticles |
| title_sort | biofilm eradication using biogenic silver nanoparticles |
| topic | biogenic nanoparticles silver nanoparticles antimicrobial antibiofilm |
| url | https://www.mdpi.com/1420-3049/25/9/2023 |
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