Study of Biofilm in Bacteria from Water Pipelines
Context: A biofilm is a layer of microorganisms contained in a matrix (slime layer), which forms on surfaces in contact with water. Their presence in drinking water pipe networks can be responsible for a wide range of water quality and operational problems. Aim: To identify the bacterial isolat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5715/12415_CE(Ra1)_F(GH)_PF1(PAK)_PFA(AK)_PF2(PAG)_u.pdf |
| Summary: | Context: A biofilm is a layer of microorganisms contained in
a matrix (slime layer), which forms on surfaces in contact with
water. Their presence in drinking water pipe networks can be
responsible for a wide range of water quality and operational
problems.
Aim: To identify the bacterial isolates, obtained from water
pipelines of kitchens, to evaluate the water quality & to study
the biofilm producing capacity of the bacterial isolates from
various sources.
Settings and Design: A prospective study using water samples
from aqua guard & pipelines to kitchens of S.C.B Medical
College hostels.
Materials and Methods: Standard biochemical procedures for
bacterial identification, multiple tube culture & MPN count to
evaluate water quality & tissue culture plate (TCP) method for
biofilm detection was followed.
Statistical analysis: STATA software version 9.2 from STATA
Corporation, College station road, 90 Houston, Texas was used
for statistical analysis.
Results: One hundred eighty seven isolates were obtained from
45 water samples cultured. The isolates were Acinetobacter spp.
(44), Pseudomonas spp.(41), Klebsiella spp.(36) & others . Biofilm
was detected in (37) 19.78 % of the isolates (95% CI 30.08%
-43.92%) including Acinetobacter spp.-10, Klebsiella spp. - 9,
Pseudomonas spp. - 9, & others, majority (34) of which were
from kitchen pipelines.
Conclusion: Water from pipeline sources was unsatisfactory
for consumption as the MPN counts were > 10. Most of the
biofilm producers were gram negative bacilli & Pseudomonas &
Acinetobacter spp. were strong (4+) biofilm producers. |
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| ISSN: | 2249-782X 0973-709X |