Study of Biofilm in Bacteria from Water Pipelines
Context: A biofilm is a layer of microorganisms contained in a matrix (slime layer), which forms on surfaces in contact with water. Their presence in drinking water pipe networks can be responsible for a wide range of water quality and operational problems. Aim: To identify the bacterial isolat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5715/12415_CE(Ra1)_F(GH)_PF1(PAK)_PFA(AK)_PF2(PAG)_u.pdf |
| _version_ | 1818132033028751360 |
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| author | Ashoka Mahapatra Nupur Padhi Dharitri Mahapatra Mamta Bhatt Debasish Sahoo Swetlina Jena Debabrata Dash Nirupama Chayani |
| author_facet | Ashoka Mahapatra Nupur Padhi Dharitri Mahapatra Mamta Bhatt Debasish Sahoo Swetlina Jena Debabrata Dash Nirupama Chayani |
| author_sort | Ashoka Mahapatra |
| collection | DOAJ |
| description | Context: A biofilm is a layer of microorganisms contained in
a matrix (slime layer), which forms on surfaces in contact with
water. Their presence in drinking water pipe networks can be
responsible for a wide range of water quality and operational
problems.
Aim: To identify the bacterial isolates, obtained from water
pipelines of kitchens, to evaluate the water quality & to study
the biofilm producing capacity of the bacterial isolates from
various sources.
Settings and Design: A prospective study using water samples
from aqua guard & pipelines to kitchens of S.C.B Medical
College hostels.
Materials and Methods: Standard biochemical procedures for
bacterial identification, multiple tube culture & MPN count to
evaluate water quality & tissue culture plate (TCP) method for
biofilm detection was followed.
Statistical analysis: STATA software version 9.2 from STATA
Corporation, College station road, 90 Houston, Texas was used
for statistical analysis.
Results: One hundred eighty seven isolates were obtained from
45 water samples cultured. The isolates were Acinetobacter spp.
(44), Pseudomonas spp.(41), Klebsiella spp.(36) & others . Biofilm
was detected in (37) 19.78 % of the isolates (95% CI 30.08%
-43.92%) including Acinetobacter spp.-10, Klebsiella spp. - 9,
Pseudomonas spp. - 9, & others, majority (34) of which were
from kitchen pipelines.
Conclusion: Water from pipeline sources was unsatisfactory
for consumption as the MPN counts were > 10. Most of the
biofilm producers were gram negative bacilli & Pseudomonas &
Acinetobacter spp. were strong (4+) biofilm producers. |
| first_indexed | 2024-12-11T08:30:23Z |
| format | Article |
| id | doaj.art-cbc53b59b91348dab77fc1f39066d27a |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-11T08:30:23Z |
| publishDate | 2015-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-cbc53b59b91348dab77fc1f39066d27a2022-12-22T01:14:29ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-03-0193DC09DC1110.7860/JCDR/2015/12415.5715Study of Biofilm in Bacteria from Water PipelinesAshoka Mahapatra0Nupur Padhi1Dharitri Mahapatra2Mamta Bhatt3Debasish Sahoo4Swetlina Jena5Debabrata Dash6Nirupama Chayani7Associate Professor, Department of Microbiology, AIIMS, Bhubaneswar S.C.B. Medical College, Cuttack, Odisha, India.Undergraduate Student, S.C.B. Medical College, Cuttack, Odisha, India.Assistant Professor, Department of Microbiology, S.C.B. Medical College, Cuttack, Odisha, India.Senior Resident, Department of Microbiology, S.C.B. Medical College, Cuttack, Odisha, India.Senior Resident, Department of Microbiology, SUM Hospital, Bhubaneswar, Odisha, India.Post Graduate Student Student, Department of Microbiology, S.C.B. Medical College, Cuttack, Odisha, India.Post Graduate Student Student, Department of Microbiology, S.C.B. Medical College, Cuttack, Odisha, India.Professor and Head, Department of Microbiology, S.C.B. Medical College, Cuttack, Odisha, India.Context: A biofilm is a layer of microorganisms contained in a matrix (slime layer), which forms on surfaces in contact with water. Their presence in drinking water pipe networks can be responsible for a wide range of water quality and operational problems. Aim: To identify the bacterial isolates, obtained from water pipelines of kitchens, to evaluate the water quality & to study the biofilm producing capacity of the bacterial isolates from various sources. Settings and Design: A prospective study using water samples from aqua guard & pipelines to kitchens of S.C.B Medical College hostels. Materials and Methods: Standard biochemical procedures for bacterial identification, multiple tube culture & MPN count to evaluate water quality & tissue culture plate (TCP) method for biofilm detection was followed. Statistical analysis: STATA software version 9.2 from STATA Corporation, College station road, 90 Houston, Texas was used for statistical analysis. Results: One hundred eighty seven isolates were obtained from 45 water samples cultured. The isolates were Acinetobacter spp. (44), Pseudomonas spp.(41), Klebsiella spp.(36) & others . Biofilm was detected in (37) 19.78 % of the isolates (95% CI 30.08% -43.92%) including Acinetobacter spp.-10, Klebsiella spp. - 9, Pseudomonas spp. - 9, & others, majority (34) of which were from kitchen pipelines. Conclusion: Water from pipeline sources was unsatisfactory for consumption as the MPN counts were > 10. Most of the biofilm producers were gram negative bacilli & Pseudomonas & Acinetobacter spp. were strong (4+) biofilm producers.https://jcdr.net/articles/PDF/5715/12415_CE(Ra1)_F(GH)_PF1(PAK)_PFA(AK)_PF2(PAG)_u.pdfbacterial aggregateshealth riskmicroorganismsplanktonic |
| spellingShingle | Ashoka Mahapatra Nupur Padhi Dharitri Mahapatra Mamta Bhatt Debasish Sahoo Swetlina Jena Debabrata Dash Nirupama Chayani Study of Biofilm in Bacteria from Water Pipelines Journal of Clinical and Diagnostic Research bacterial aggregates health risk microorganisms planktonic |
| title | Study of Biofilm in Bacteria from Water Pipelines |
| title_full | Study of Biofilm in Bacteria from Water Pipelines |
| title_fullStr | Study of Biofilm in Bacteria from Water Pipelines |
| title_full_unstemmed | Study of Biofilm in Bacteria from Water Pipelines |
| title_short | Study of Biofilm in Bacteria from Water Pipelines |
| title_sort | study of biofilm in bacteria from water pipelines |
| topic | bacterial aggregates health risk microorganisms planktonic |
| url | https://jcdr.net/articles/PDF/5715/12415_CE(Ra1)_F(GH)_PF1(PAK)_PFA(AK)_PF2(PAG)_u.pdf |
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