Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism
Research has proven that light treatment, specifically red light radiation, can provide more clinical benefits to human health. Our investigation was firstly conducted to characterize the tissue morphology of mouse breast post 660 nm laser radiation with low power and long-term exposure. RNA sequenc...
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MDPI AG
2022-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/10/1389 |
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| author | Qiyang Xiao Lijing Wang Juling Zhang Xinyu Zhong Zhou Guo Jiahao Yu Yuanyuan Ma Haigang Wu |
| author_facet | Qiyang Xiao Lijing Wang Juling Zhang Xinyu Zhong Zhou Guo Jiahao Yu Yuanyuan Ma Haigang Wu |
| author_sort | Qiyang Xiao |
| collection | DOAJ |
| description | Research has proven that light treatment, specifically red light radiation, can provide more clinical benefits to human health. Our investigation was firstly conducted to characterize the tissue morphology of mouse breast post 660 nm laser radiation with low power and long-term exposure. RNA sequencing results revealed that light exposure with a higher intervention dosage could cause a number of differentially expressed genes compared with a low intervention dosage. Gene ontology analysis, protein–protein interaction network analysis, and gene set enrichment analysis results suggested that 660 nm light exposure can activate more transcription-related pathways in HC11 breast epithelial cells, and these pathways may involve modulating critical gene expression. To consider the critical role of the Wnt/T-catenin pathway in light-induced modulation, we hypothesized that this pathway might play a major role in response to 660 nm light exposure. To validate our hypothesis, we conducted qRT-PCR, immunofluorescence staining, and Western blot assays, and relative results corroborated that laser radiation could promote expression levels of β-catenin and relative phosphorylation. Significant changes in metabolites and pathway analysis revealed that 660 nm laser could affect nucleotide metabolism by regulating purine metabolism. These findings suggest that the Wnt/β-catenin pathway may be the major sensor for 660 nm laser radiation, and it may be helpful to rescue drawbacks or side effects of 660 nm light exposure through relative interventional agents. |
| first_indexed | 2024-03-09T20:37:20Z |
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| id | doaj.art-cbc62fc11ec94081a165f457e8fb5667 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T20:37:20Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-cbc62fc11ec94081a165f457e8fb56672023-11-23T23:07:52ZengMDPI AGBiomolecules2218-273X2022-09-011210138910.3390/biom12101389Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid MetabolismQiyang Xiao0Lijing Wang1Juling Zhang2Xinyu Zhong3Zhou Guo4Jiahao Yu5Yuanyuan Ma6Haigang Wu7School of Artificial Intelligence, Henan University, Zhengzhou 450046, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaCenter for Faculty Development, South China Normal University, Guangzhou 510631, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaShandong Zhongbaokang Medical Implements Co., Ltd., Zibo 255000, ChinaSchool of Pharmacy, Henan University, Kaifeng 475000, ChinaSchool of Artificial Intelligence, Henan University, Zhengzhou 450046, ChinaResearch has proven that light treatment, specifically red light radiation, can provide more clinical benefits to human health. Our investigation was firstly conducted to characterize the tissue morphology of mouse breast post 660 nm laser radiation with low power and long-term exposure. RNA sequencing results revealed that light exposure with a higher intervention dosage could cause a number of differentially expressed genes compared with a low intervention dosage. Gene ontology analysis, protein–protein interaction network analysis, and gene set enrichment analysis results suggested that 660 nm light exposure can activate more transcription-related pathways in HC11 breast epithelial cells, and these pathways may involve modulating critical gene expression. To consider the critical role of the Wnt/T-catenin pathway in light-induced modulation, we hypothesized that this pathway might play a major role in response to 660 nm light exposure. To validate our hypothesis, we conducted qRT-PCR, immunofluorescence staining, and Western blot assays, and relative results corroborated that laser radiation could promote expression levels of β-catenin and relative phosphorylation. Significant changes in metabolites and pathway analysis revealed that 660 nm laser could affect nucleotide metabolism by regulating purine metabolism. These findings suggest that the Wnt/β-catenin pathway may be the major sensor for 660 nm laser radiation, and it may be helpful to rescue drawbacks or side effects of 660 nm light exposure through relative interventional agents.https://www.mdpi.com/2218-273X/12/10/1389breastRNA sequencingmetabolitepurine metabolismWnt/β-catenin |
| spellingShingle | Qiyang Xiao Lijing Wang Juling Zhang Xinyu Zhong Zhou Guo Jiahao Yu Yuanyuan Ma Haigang Wu Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism Biomolecules breast RNA sequencing metabolite purine metabolism Wnt/β-catenin |
| title | Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism |
| title_full | Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism |
| title_fullStr | Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism |
| title_full_unstemmed | Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism |
| title_short | Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism |
| title_sort | activation of wnt β catenin signaling involves 660 nm laser radiation on epithelium and modulates lipid metabolism |
| topic | breast RNA sequencing metabolite purine metabolism Wnt/β-catenin |
| url | https://www.mdpi.com/2218-273X/12/10/1389 |
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