Pulse-shaping-based two-photon FRET microscopy

Pulse-shaping-based two-photon FRET microscopy

We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.

Bibliographic Details
Main Authors:	Swanson J. A., Straight S. W., Cai D., Brenner M. H., Ogilvie J. P.
Format:	Article
Language:	English
Published:	EDP Sciences 2013-03-01
Series:	EPJ Web of Conferences
Online Access:	http://dx.doi.org/10.1051/epjconf/20134111009

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