Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.)
Sunflower is an important oilseed crop across the world. It is considered as a moderately drought tolerant plant, however, its yield is still negatively affected by drought stress. Improving drought tolerance is of the outmost important for breeding. Although several studies have documented the rela...
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PeerJ Inc.
2023-05-01
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| author | Huimin Shi Yang Wu Liuxi Yi Haibo Hu Feiyan Su Yanxia Wang Dandan Li Jianhua Hou |
| author_facet | Huimin Shi Yang Wu Liuxi Yi Haibo Hu Feiyan Su Yanxia Wang Dandan Li Jianhua Hou |
| author_sort | Huimin Shi |
| collection | DOAJ |
| description | Sunflower is an important oilseed crop across the world. It is considered as a moderately drought tolerant plant, however, its yield is still negatively affected by drought stress. Improving drought tolerance is of the outmost important for breeding. Although several studies have documented the relationship between the sunflower phenotype and genotype under drought stress, but relatively few studies have simultaneously investigated the molecular mechanisms of drought tolerance in the sunflower at different growth stages. In this study, we conducted quantitative trait locus (QTL) analysis for different sunflower traits during the germination and seedling stages. Eighteen phenotypic traits were evaluated under well-watered and drought stress conditions. We determined that the germination rate, germination potential, germination index, and root-to-shoot ratio can be used as effective indexes for drought tolerance selection and breeding. A total of 33 QTLs were identified on eight chromosomes (PVE: 0.016%–10.712% with LOD: 2.017–7.439). Within the confidence interval of the QTL, we identified 60 putative drought-related genes. Four genes located on chromosome 13 may function in both germination and seedling stages for drought response. Genes LOC110898128, LOC110898092, LOC110898071, and LOC110898072 were annotated as aquaporin SIP1-2-like, cytochrome P450 94C1, GABA transporter 1-like, and GABA transporter 1-like isoform X2, respectively. These genes will be used for further functional validation. This study provides insight into the molecular mechanisms of the sunflower’s in response to drought stress. At the same time, it lays a foundation for sunflower drought tolerance breeding and genetic improvement. |
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| last_indexed | 2024-03-09T06:28:13Z |
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| spelling | doaj.art-cbcaa343ddf7438b89d59c438bf46e652023-12-03T11:13:48ZengPeerJ Inc.PeerJ2167-83592023-05-0111e1527510.7717/peerj.15275Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.)Huimin Shi0Yang Wu1Liuxi Yi2Haibo Hu3Feiyan Su4Yanxia Wang5Dandan Li6Jianhua Hou7Inner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaInner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaInner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaInner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaInner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaInner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaInner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaInner Mongolia Agricultural University, College of Agriculture, Huhhot, ChinaSunflower is an important oilseed crop across the world. It is considered as a moderately drought tolerant plant, however, its yield is still negatively affected by drought stress. Improving drought tolerance is of the outmost important for breeding. Although several studies have documented the relationship between the sunflower phenotype and genotype under drought stress, but relatively few studies have simultaneously investigated the molecular mechanisms of drought tolerance in the sunflower at different growth stages. In this study, we conducted quantitative trait locus (QTL) analysis for different sunflower traits during the germination and seedling stages. Eighteen phenotypic traits were evaluated under well-watered and drought stress conditions. We determined that the germination rate, germination potential, germination index, and root-to-shoot ratio can be used as effective indexes for drought tolerance selection and breeding. A total of 33 QTLs were identified on eight chromosomes (PVE: 0.016%–10.712% with LOD: 2.017–7.439). Within the confidence interval of the QTL, we identified 60 putative drought-related genes. Four genes located on chromosome 13 may function in both germination and seedling stages for drought response. Genes LOC110898128, LOC110898092, LOC110898071, and LOC110898072 were annotated as aquaporin SIP1-2-like, cytochrome P450 94C1, GABA transporter 1-like, and GABA transporter 1-like isoform X2, respectively. These genes will be used for further functional validation. This study provides insight into the molecular mechanisms of the sunflower’s in response to drought stress. At the same time, it lays a foundation for sunflower drought tolerance breeding and genetic improvement.https://peerj.com/articles/15275.pdfSunflowerGermination and seedling stagesDrought toleranceSingle-nucleotide polymorphisms (SNPs)Quantitative trait locus (QTL)Candidate genes |
| spellingShingle | Huimin Shi Yang Wu Liuxi Yi Haibo Hu Feiyan Su Yanxia Wang Dandan Li Jianhua Hou Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.) PeerJ Sunflower Germination and seedling stages Drought tolerance Single-nucleotide polymorphisms (SNPs) Quantitative trait locus (QTL) Candidate genes |
| title | Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.) |
| title_full | Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.) |
| title_fullStr | Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.) |
| title_full_unstemmed | Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.) |
| title_short | Analysis of QTL mapping for germination and seedling response to drought stress in sunflower (Helianthus annuus L.) |
| title_sort | analysis of qtl mapping for germination and seedling response to drought stress in sunflower helianthus annuus l |
| topic | Sunflower Germination and seedling stages Drought tolerance Single-nucleotide polymorphisms (SNPs) Quantitative trait locus (QTL) Candidate genes |
| url | https://peerj.com/articles/15275.pdf |
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