Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies?
The detection of anti-phosphatidylethanolamine autoantibodies (aPEs) has been proposed to improve the diagnosis and management of patients presenting clinical manifestations of antiphospholipid syndrome (APS), such as thrombosis, and who are persistently negative for conventional markers. After sele...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/5/1297 |
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| author | Xavier Heim Daniel Bertin Noémie Resseguier Abdelouahab Beziane Audrey Metral Alexandre Brodovitch Régis Guieu Jean-Guillaume Steinberg Marcel Blot-Chabaud Pierre-Emmanuel Morange Jean-Louis Mege Nathalie Bardin |
| author_facet | Xavier Heim Daniel Bertin Noémie Resseguier Abdelouahab Beziane Audrey Metral Alexandre Brodovitch Régis Guieu Jean-Guillaume Steinberg Marcel Blot-Chabaud Pierre-Emmanuel Morange Jean-Louis Mege Nathalie Bardin |
| author_sort | Xavier Heim |
| collection | DOAJ |
| description | The detection of anti-phosphatidylethanolamine autoantibodies (aPEs) has been proposed to improve the diagnosis and management of patients presenting clinical manifestations of antiphospholipid syndrome (APS), such as thrombosis, and who are persistently negative for conventional markers. After selecting the most specific ELISA for their detection, we evidenced the interest of aPEs in the exploration of thrombosis when APS conventional markers were negative through a 1-year retrospective study including 1131 consecutive patients routinely tested for aPEs. To validate this result, we assessed aPEs in a newly selected population of 77 patients with unexplained deep vein thrombosis (DVT). With a total prevalence of 19.5%, we confirmed the interest of aPE detection in patients with unexplained DVT who were devoid of other aPLs markers. Since endosomal compartment, a source of ROS production, has been recently identified as the cellular target of aPEs in vitro, we then investigated an association between aPE positivity and reactive oxygen species (ROS) production by measuring the production of thiobarbituric acid-reactive substances. We showed, for the first time, a significant association between aPE positivity and systemic ROS production in patients which led us to hypothesize a new mechanism of action of aPEs in thrombosis through a signaling related to oxidative stress. |
| first_indexed | 2024-03-09T20:35:21Z |
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| id | doaj.art-cbcef17dff4e47dcb1c67f9cd054e4d7 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T20:35:21Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-cbcef17dff4e47dcb1c67f9cd054e4d72023-11-23T23:13:46ZengMDPI AGJournal of Clinical Medicine2077-03832022-02-01115129710.3390/jcm11051297Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies?Xavier Heim0Daniel Bertin1Noémie Resseguier2Abdelouahab Beziane3Audrey Metral4Alexandre Brodovitch5Régis Guieu6Jean-Guillaume Steinberg7Marcel Blot-Chabaud8Pierre-Emmanuel Morange9Jean-Louis Mege10Nathalie Bardin11Service d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13005 Marseille, FranceService d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13005 Marseille, FranceService de Santé Publique, Unité de Recherche EA 3279, Aix-Marseille Université, 13005 Marseille, FranceService d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13005 Marseille, FranceService d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13005 Marseille, FranceService d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13005 Marseille, FranceCenter for CardioVascular and Nutrition Research C2VN, INSERM, INRAE Faculty of Pharmacy, Timone Campus, Aix-Marseille University, 13005 Marseille, FranceCenter for CardioVascular and Nutrition Research C2VN, INSERM, INRAE Faculty of Pharmacy, Timone Campus, Aix-Marseille University, 13005 Marseille, FranceCenter for CardioVascular and Nutrition Research C2VN, INSERM, INRAE Faculty of Pharmacy, Timone Campus, Aix-Marseille University, 13005 Marseille, FranceCenter for CardioVascular and Nutrition Research C2VN, INSERM, INRAE Faculty of Pharmacy, Timone Campus, Aix-Marseille University, 13005 Marseille, FranceService d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13005 Marseille, FranceService d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13005 Marseille, FranceThe detection of anti-phosphatidylethanolamine autoantibodies (aPEs) has been proposed to improve the diagnosis and management of patients presenting clinical manifestations of antiphospholipid syndrome (APS), such as thrombosis, and who are persistently negative for conventional markers. After selecting the most specific ELISA for their detection, we evidenced the interest of aPEs in the exploration of thrombosis when APS conventional markers were negative through a 1-year retrospective study including 1131 consecutive patients routinely tested for aPEs. To validate this result, we assessed aPEs in a newly selected population of 77 patients with unexplained deep vein thrombosis (DVT). With a total prevalence of 19.5%, we confirmed the interest of aPE detection in patients with unexplained DVT who were devoid of other aPLs markers. Since endosomal compartment, a source of ROS production, has been recently identified as the cellular target of aPEs in vitro, we then investigated an association between aPE positivity and reactive oxygen species (ROS) production by measuring the production of thiobarbituric acid-reactive substances. We showed, for the first time, a significant association between aPE positivity and systemic ROS production in patients which led us to hypothesize a new mechanism of action of aPEs in thrombosis through a signaling related to oxidative stress.https://www.mdpi.com/2077-0383/11/5/1297reactive oxygen species (ROS)thrombosisanti-phosphatidylethanolamine autoantibodiesELISAantiphospholipid syndromethiobarbituric acid-reactive substances (TBARs) |
| spellingShingle | Xavier Heim Daniel Bertin Noémie Resseguier Abdelouahab Beziane Audrey Metral Alexandre Brodovitch Régis Guieu Jean-Guillaume Steinberg Marcel Blot-Chabaud Pierre-Emmanuel Morange Jean-Louis Mege Nathalie Bardin Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies? Journal of Clinical Medicine reactive oxygen species (ROS) thrombosis anti-phosphatidylethanolamine autoantibodies ELISA antiphospholipid syndrome thiobarbituric acid-reactive substances (TBARs) |
| title | Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies? |
| title_full | Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies? |
| title_fullStr | Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies? |
| title_full_unstemmed | Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies? |
| title_short | Is Oxidative Stress an Emerging Player in the Thrombosis of Patients with Anti-Phosphatidylethanolamine Autoantibodies? |
| title_sort | is oxidative stress an emerging player in the thrombosis of patients with anti phosphatidylethanolamine autoantibodies |
| topic | reactive oxygen species (ROS) thrombosis anti-phosphatidylethanolamine autoantibodies ELISA antiphospholipid syndrome thiobarbituric acid-reactive substances (TBARs) |
| url | https://www.mdpi.com/2077-0383/11/5/1297 |
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