α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins
Cortical cytoskeletal proteins are significant in controlling various cellular mechanisms such as migration, cell adhesion, intercellular attachment, cellular signaling, exo- and endocytosis and plasma membrane integrity, stability and flexibility. Our earlier studies involving in vitro and ex vivo...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/5/750 |
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| author | Jamuna S. Sreeja Athira Jyothy Suparna Sengupta |
| author_facet | Jamuna S. Sreeja Athira Jyothy Suparna Sengupta |
| author_sort | Jamuna S. Sreeja |
| collection | DOAJ |
| description | Cortical cytoskeletal proteins are significant in controlling various cellular mechanisms such as migration, cell adhesion, intercellular attachment, cellular signaling, exo- and endocytosis and plasma membrane integrity, stability and flexibility. Our earlier studies involving in vitro and ex vivo approaches led us to identify certain undiscovered characteristics of α-fodrin, a prominent cortical protein. The conventional functions attributed to this protein mainly support the plasma membrane. In the present study, we utilized a global protein expression analysis approach to detect underexplored functions of this protein. We report that downregulation of α-fodrin in glioblastoma cells, U-251 MG, results in upregulation of genes affecting the regulation of the cytoskeleton, cell cycle and apoptosis. Interestingly, certain key microtubule kinesins such as KIF23, KIF2B and KIF3C are downregulated upon α-fodrin depletion, as validated by real-time PCR studies. |
| first_indexed | 2024-03-10T11:21:24Z |
| format | Article |
| id | doaj.art-cbd4f7df799d4b519b5927d4362f33da |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:21:24Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-cbd4f7df799d4b519b5927d4362f33da2023-11-21T20:00:19ZengMDPI AGGenes2073-44252021-05-0112575010.3390/genes12050750α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule KinesinsJamuna S. Sreeja0Athira Jyothy1Suparna Sengupta2Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, University of Kerala, Thiruvananthapuram 695014, IndiaCancer Research Program, Rajiv Gandhi Centre for Biotechnology, University of Kerala, Thiruvananthapuram 695014, IndiaCancer Research Program, Rajiv Gandhi Centre for Biotechnology, University of Kerala, Thiruvananthapuram 695014, IndiaCortical cytoskeletal proteins are significant in controlling various cellular mechanisms such as migration, cell adhesion, intercellular attachment, cellular signaling, exo- and endocytosis and plasma membrane integrity, stability and flexibility. Our earlier studies involving in vitro and ex vivo approaches led us to identify certain undiscovered characteristics of α-fodrin, a prominent cortical protein. The conventional functions attributed to this protein mainly support the plasma membrane. In the present study, we utilized a global protein expression analysis approach to detect underexplored functions of this protein. We report that downregulation of α-fodrin in glioblastoma cells, U-251 MG, results in upregulation of genes affecting the regulation of the cytoskeleton, cell cycle and apoptosis. Interestingly, certain key microtubule kinesins such as KIF23, KIF2B and KIF3C are downregulated upon α-fodrin depletion, as validated by real-time PCR studies.https://www.mdpi.com/2073-4425/12/5/750fodrinspectrinkinesinsLC-MSproteomics |
| spellingShingle | Jamuna S. Sreeja Athira Jyothy Suparna Sengupta α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins Genes fodrin spectrin kinesins LC-MS proteomics |
| title | α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins |
| title_full | α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins |
| title_fullStr | α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins |
| title_full_unstemmed | α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins |
| title_short | α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins |
| title_sort | α fodrin in cytoskeletal organization and the activity of certain key microtubule kinesins |
| topic | fodrin spectrin kinesins LC-MS proteomics |
| url | https://www.mdpi.com/2073-4425/12/5/750 |
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