Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of Iraq
Background: Ovine pulmonary adenocarcinoma (OPA), caused by Jaagsiekte sheep retrovirus (JSRV), is a contagious neoplastic disease of sheep characterized by chronic respiratory signs and induce the transformation of secretory epithelial cells of the distal respiratory tract. Aim: This research aim...
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| Format: | Article |
| Language: | English |
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Tripoli University
2022-04-01
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| Series: | Open Veterinary Journal |
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| Online Access: | https://www.openveterinaryjournal.com/OVJ-2021-12-220%20F.A.%20Abass%20and%20Y.I.%20Khudhair.pdf |
| _version_ | 1818186809342951424 |
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| author | Falah Abd Abass Yahia Ismail Khudhair |
| author_facet | Falah Abd Abass Yahia Ismail Khudhair |
| author_sort | Falah Abd Abass |
| collection | DOAJ |
| description | Background: Ovine pulmonary adenocarcinoma (OPA), caused by Jaagsiekte sheep retrovirus (JSRV), is a contagious neoplastic disease of sheep characterized by chronic respiratory signs and induce the transformation of secretory epithelial cells of the distal respiratory tract.
Aim: This research aims to perform a clinical, epidemiological and molecular studies with evaluation of some predisposing factors at the herd level of OPA infection in sheep in Al-Qadisiyah province, Iraq.
Methods: a first step of the study was undertaken to evaluate the clinical cases of OPA in naturally infected sheep and correlation with observing respiratory signs. Seventy-five sheep with chronic respiratory signs were examined clinically, and by molecular and sequences analysis. The second step was the epidemiological part that was performed on 195 random-selected animals from 30 flocks, with prevalence rate based on the PCR, and sex, age, and size of flocks was assessed, as well as macro-microscopic features of the neoplastic lung. Deep nasal swabs and nasal secretion were collected from all animals. RNA extraction and RT-PCR were done.
Results: The results showed that 12 (16%) samples were positive to OPA, based on env gene-specific primers. Nucleotides sequences of partial 545 bp of the env gene were shown (0.07-0.12) variation from global strains presented in NCBI database. The prevalence rate of OPA was 21/195 (10.76 %) with PCR. The epidemiological factors analysis showed that there was no effect of sex and herd size on the prevalence rates (P≥ 0.01), whereas the age was significantly affected and the age of 2-4 years was more susceptible (P≥ 0.01). A gross and microscopic examinations were discussed with the confirmation of an OPA infection.
Conclusion: The current study provides useful data about clinical and epidemiological features of JSRV that circulating in sheep of Iraq, and concludes that epidemiological studies and disease control may require multi-diagnostic assays. |
| first_indexed | 2024-12-11T23:01:02Z |
| format | Article |
| id | doaj.art-cbd59077b87f4d3b9726be79c1af75d6 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 2218-6050 |
| language | English |
| last_indexed | 2024-12-11T23:01:02Z |
| publishDate | 2022-04-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-cbd59077b87f4d3b9726be79c1af75d62022-12-22T00:47:03ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502022-04-0112226427210.5455/OVJ.2022.v12.i2.15Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of IraqFalah Abd Abass0https://orcid.org/0000-0002-8029-9369Yahia Ismail Khudhair1https://orcid.org/0000-0002-0816-1883Veterinary Hospital, Al-Diwaniyah, Al-Qadisiyah, IraqDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, IraqBackground: Ovine pulmonary adenocarcinoma (OPA), caused by Jaagsiekte sheep retrovirus (JSRV), is a contagious neoplastic disease of sheep characterized by chronic respiratory signs and induce the transformation of secretory epithelial cells of the distal respiratory tract. Aim: This research aims to perform a clinical, epidemiological and molecular studies with evaluation of some predisposing factors at the herd level of OPA infection in sheep in Al-Qadisiyah province, Iraq. Methods: a first step of the study was undertaken to evaluate the clinical cases of OPA in naturally infected sheep and correlation with observing respiratory signs. Seventy-five sheep with chronic respiratory signs were examined clinically, and by molecular and sequences analysis. The second step was the epidemiological part that was performed on 195 random-selected animals from 30 flocks, with prevalence rate based on the PCR, and sex, age, and size of flocks was assessed, as well as macro-microscopic features of the neoplastic lung. Deep nasal swabs and nasal secretion were collected from all animals. RNA extraction and RT-PCR were done. Results: The results showed that 12 (16%) samples were positive to OPA, based on env gene-specific primers. Nucleotides sequences of partial 545 bp of the env gene were shown (0.07-0.12) variation from global strains presented in NCBI database. The prevalence rate of OPA was 21/195 (10.76 %) with PCR. The epidemiological factors analysis showed that there was no effect of sex and herd size on the prevalence rates (P≥ 0.01), whereas the age was significantly affected and the age of 2-4 years was more susceptible (P≥ 0.01). A gross and microscopic examinations were discussed with the confirmation of an OPA infection. Conclusion: The current study provides useful data about clinical and epidemiological features of JSRV that circulating in sheep of Iraq, and concludes that epidemiological studies and disease control may require multi-diagnostic assays. https://www.openveterinaryjournal.com/OVJ-2021-12-220%20F.A.%20Abass%20and%20Y.I.%20Khudhair.pdfadenocarcinomaopaovinepulmonaryiraq |
| spellingShingle | Falah Abd Abass Yahia Ismail Khudhair Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of Iraq Open Veterinary Journal adenocarcinoma opa ovine pulmonary iraq |
| title | Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of Iraq |
| title_full | Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of Iraq |
| title_fullStr | Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of Iraq |
| title_full_unstemmed | Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of Iraq |
| title_short | Clinical, molecular, and pathological investigations of ovine pulmonary adenocarcinoma in the middle of Iraq |
| title_sort | clinical molecular and pathological investigations of ovine pulmonary adenocarcinoma in the middle of iraq |
| topic | adenocarcinoma opa ovine pulmonary iraq |
| url | https://www.openveterinaryjournal.com/OVJ-2021-12-220%20F.A.%20Abass%20and%20Y.I.%20Khudhair.pdf |
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