Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy
A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, d...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | EPJ Web of Conferences |
| Online Access: | https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_03009.pdf |
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| author | Kariman Behjat S. Nardini Alessandra Grassi Marco Marini Mario Conci Claudio Bouzin Margaux Collini Maddalena Raimondi Manuela T. Chirico Giuseppe Osellame Roberto Cerullo Giulio Martínez Vázquez Rebeca |
| author_facet | Kariman Behjat S. Nardini Alessandra Grassi Marco Marini Mario Conci Claudio Bouzin Margaux Collini Maddalena Raimondi Manuela T. Chirico Giuseppe Osellame Roberto Cerullo Giulio Martínez Vázquez Rebeca |
| author_sort | Kariman Behjat S. |
| collection | DOAJ |
| description | A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. A low numerical aperture objective lens coupled to high dioptric power microlenses, implanted in the tissue, can be beneficial for the reduction of optical aberrations. In this context, we fabricated a system of plano-convex microlenses and microscaffolds on a single chip by means of two-photon polymerization), to be used for non-linear imaging of biological specimens. |
| first_indexed | 2024-03-11T12:14:07Z |
| format | Article |
| id | doaj.art-cbda8afa26f946a39efbb7904c6d8905 |
| institution | Directory Open Access Journal |
| issn | 2100-014X |
| language | English |
| last_indexed | 2024-03-11T12:14:07Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | EPJ Web of Conferences |
| spelling | doaj.art-cbda8afa26f946a39efbb7904c6d89052023-11-07T10:20:40ZengEDP SciencesEPJ Web of Conferences2100-014X2023-01-012870300910.1051/epjconf/202328703009epjconf_eosam2023_03009Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation MicroscopyKariman Behjat S.0Nardini Alessandra1Grassi Marco2Marini Mario3Conci Claudio4Bouzin Margaux5Collini Maddalena6Raimondi Manuela T.7Chirico Giuseppe8Osellame Roberto9Cerullo Giulio10Martínez Vázquez Rebeca11Department of Physics, Politecnico di MilanoDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di MilanoDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di MilanoDepartment of Physics, Università degli Studi di Milano-BicoccaDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di MilanoDepartment of Physics, Università degli Studi di Milano-BicoccaDepartment of Physics, Università degli Studi di Milano-BicoccaDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di MilanoDepartment of Physics, Università degli Studi di Milano-BicoccaInstitute for Photonics and Nanotechnologies (IFN), CNRDepartment of Physics, Politecnico di MilanoInstitute for Photonics and Nanotechnologies (IFN), CNRA recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. A low numerical aperture objective lens coupled to high dioptric power microlenses, implanted in the tissue, can be beneficial for the reduction of optical aberrations. In this context, we fabricated a system of plano-convex microlenses and microscaffolds on a single chip by means of two-photon polymerization), to be used for non-linear imaging of biological specimens.https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_03009.pdf |
| spellingShingle | Kariman Behjat S. Nardini Alessandra Grassi Marco Marini Mario Conci Claudio Bouzin Margaux Collini Maddalena Raimondi Manuela T. Chirico Giuseppe Osellame Roberto Cerullo Giulio Martínez Vázquez Rebeca Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy EPJ Web of Conferences |
| title | Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy |
| title_full | Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy |
| title_fullStr | Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy |
| title_full_unstemmed | Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy |
| title_short | Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy |
| title_sort | fabrication of plano convex microlenses using two photon polymerization for bioimaging with non linear excitation microscopy |
| url | https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_03009.pdf |
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