The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC
Abstract Background Hepatocellular carcinoma (HCC) is a common, serious malignancy with a dismal prognosis. As HCC is frequently missed in its early stages, non-invasive early detection is urgently needed. The purpose of this study was to evaluate the possible utility of circulating miRNA-29b1, matr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-10-01
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| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43042-023-00434-w |
| _version_ | 1827634067709886464 |
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| author | Marwa Sayed Abdel-Tawab Hanan Fouad Doaa Mahmoud Khalil Saeed Shaaban Shaymaa Nafady Hanan Hosni Moawad Heba Ahmed Mostafa Soha Mahmoud Abdel-Salam Naglaa Adly Abd Elazeem Alaa A. Mohamed |
| author_facet | Marwa Sayed Abdel-Tawab Hanan Fouad Doaa Mahmoud Khalil Saeed Shaaban Shaymaa Nafady Hanan Hosni Moawad Heba Ahmed Mostafa Soha Mahmoud Abdel-Salam Naglaa Adly Abd Elazeem Alaa A. Mohamed |
| author_sort | Marwa Sayed Abdel-Tawab |
| collection | DOAJ |
| description | Abstract Background Hepatocellular carcinoma (HCC) is a common, serious malignancy with a dismal prognosis. As HCC is frequently missed in its early stages, non-invasive early detection is urgently needed. The purpose of this study was to evaluate the possible utility of circulating miRNA-29b1, matrix metalloproteinases (MMPs)-2 and -9 mRNAs, and proteins as diagnostic and predictive biomarkers for HCC. Subjects and methods This study included 92 subjects, including 52 patients with HCC at various stages and grades and 40 healthy subjects as controls. RT-PCR was used to detect circulating miRNA-29b1, MMPs-2, and 9 mRNAs, while ELISA was used to detect AFP, MMPs-2, and 9 proteins in the participants’ blood. Results When HCC patients were compared to controls, there were significant increases in the levels of MMPs-2, 9 mRNAs, and proteins, and a significant drop in the levels of miRNA-29b1. There were no significant variations in the levels of miRNA-29b1, mRNAs, and MMP-2 and -9 proteins in advanced HCC. There were negative associations between miRNA-29b1 and MMPs-2, 9 mRNAs, and proteins, implying overlapping molecular microRNA-mediated mechanisms that control MMPs that should be investigated further in the future. The levels of miRNA-29b1, MMPs-2, 9 mRNAs, and proteins indicated significant sensitivity and specificity in the early identification of HCC. Conclusion MMP-2, 9 mRNAs, and proteins may be employed as diagnostic but not prognostic biomarkers in HCC. miRNA-29b1 may play a protective role in HCC. An overlapping molecular microRNA-29b1-mediated pathway that may control MMPs-2 and 9 requires further experimental investigation in the future. |
| first_indexed | 2024-03-09T15:08:15Z |
| format | Article |
| id | doaj.art-cbdb2bb201f1458eba2b1faab00cdcc5 |
| institution | Directory Open Access Journal |
| issn | 2090-2441 |
| language | English |
| last_indexed | 2024-03-09T15:08:15Z |
| publishDate | 2023-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-cbdb2bb201f1458eba2b1faab00cdcc52023-11-26T13:31:58ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412023-10-0124111010.1186/s43042-023-00434-wThe role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCCMarwa Sayed Abdel-Tawab0Hanan Fouad1Doaa Mahmoud Khalil2Saeed Shaaban3Shaymaa Nafady4Hanan Hosni Moawad5Heba Ahmed Mostafa6Soha Mahmoud Abdel-Salam7Naglaa Adly Abd Elazeem8Alaa A. Mohamed9Medical Biochemistry Department, Faculty of Medicine, Beni-Suef UniversityMedical Biochemistry Department, Faculty of Medicine, Cairo UniversityDepartment of Public Health and Community Medicine, Faculty of Medicine, Beni-Suef UniversityDepartment of Clinical Oncology, Faculty of Medicine, Beni-Suef UniversityDepartment of Gastroenterology, Hepatology and Infectious Diseases, Faculty of Medicine, Beni-Suef UniversityDepartment of Clinical and Chemical Pathology, Faculty of Medicine, Beni-Suef UniversityDepartment of Radiology, Faculty of Medicine, Beni-Suef UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Suez UniversityMedical Biochemistry Department, Faculty of Medicine, Beni-Suef UniversityMedical Biochemistry Department, Faculty of Medicine, Beni-Suef UniversityAbstract Background Hepatocellular carcinoma (HCC) is a common, serious malignancy with a dismal prognosis. As HCC is frequently missed in its early stages, non-invasive early detection is urgently needed. The purpose of this study was to evaluate the possible utility of circulating miRNA-29b1, matrix metalloproteinases (MMPs)-2 and -9 mRNAs, and proteins as diagnostic and predictive biomarkers for HCC. Subjects and methods This study included 92 subjects, including 52 patients with HCC at various stages and grades and 40 healthy subjects as controls. RT-PCR was used to detect circulating miRNA-29b1, MMPs-2, and 9 mRNAs, while ELISA was used to detect AFP, MMPs-2, and 9 proteins in the participants’ blood. Results When HCC patients were compared to controls, there were significant increases in the levels of MMPs-2, 9 mRNAs, and proteins, and a significant drop in the levels of miRNA-29b1. There were no significant variations in the levels of miRNA-29b1, mRNAs, and MMP-2 and -9 proteins in advanced HCC. There were negative associations between miRNA-29b1 and MMPs-2, 9 mRNAs, and proteins, implying overlapping molecular microRNA-mediated mechanisms that control MMPs that should be investigated further in the future. The levels of miRNA-29b1, MMPs-2, 9 mRNAs, and proteins indicated significant sensitivity and specificity in the early identification of HCC. Conclusion MMP-2, 9 mRNAs, and proteins may be employed as diagnostic but not prognostic biomarkers in HCC. miRNA-29b1 may play a protective role in HCC. An overlapping molecular microRNA-29b1-mediated pathway that may control MMPs-2 and 9 requires further experimental investigation in the future.https://doi.org/10.1186/s43042-023-00434-wHCCGene expressionmiRNA-29b1MMP-2MMP-9Protein expression |
| spellingShingle | Marwa Sayed Abdel-Tawab Hanan Fouad Doaa Mahmoud Khalil Saeed Shaaban Shaymaa Nafady Hanan Hosni Moawad Heba Ahmed Mostafa Soha Mahmoud Abdel-Salam Naglaa Adly Abd Elazeem Alaa A. Mohamed The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC Egyptian Journal of Medical Human Genetics HCC Gene expression miRNA-29b1 MMP-2 MMP-9 Protein expression |
| title | The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC |
| title_full | The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC |
| title_fullStr | The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC |
| title_full_unstemmed | The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC |
| title_short | The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC |
| title_sort | role of mirna 29b1 mmp 2 mmp 9 mrnas and proteins in early diagnosis of hcc |
| topic | HCC Gene expression miRNA-29b1 MMP-2 MMP-9 Protein expression |
| url | https://doi.org/10.1186/s43042-023-00434-w |
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