Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract Infections
Introduction: Studies indicate that as observed in most bacteria, Escherichia coli isolated from community-acquired urinary tract infections has also become resistant to most antibiotics and is increasingly becoming so as time passes. In this study, we aimed to investigate extended-spectrum beta-lac...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2012-12-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
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| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2012-17-04-181-186.pdf |
| _version_ | 1797906840187568128 |
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| author | Laser ŞANAL |
| author_facet | Laser ŞANAL |
| author_sort | Laser ŞANAL |
| collection | DOAJ |
| description | Introduction: Studies indicate that as observed in most bacteria, Escherichia coli isolated from community-acquired urinary tract infections has also become resistant to most antibiotics and is increasingly becoming so as time passes. In this study, we aimed to investigate extended-spectrum beta-lactamase (ESBL) ratios in E. coli strains isolated from community-acquired urinary tract infections and the susceptibilities of these strains to fosfomycin trometamol and other antibiotics. Materials and Methods: In this study, the urinary cultures of 3257 outpatients who applied to Ankara Occupational Diseases Hospital between 01 January 2012-31 July 2012 with urinary tract complaints were examined. The urine specimens collected from patients were inoculated to 5% sheep blood agar and Eosin-Methylene-Blue (EMB) agar, and incubated at 37°C for 18-24 hours. The antibiotic susceptibility testing was performed by Kirby Bauer disk diffusion method in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. ESBL presence in E. coli strains isolated from urine samples was investigated by double disk synergy test. Results: Growth was detected in 443 (13.6%) of the 3257 patients from whom urinary cultures were requested. The most frequent bacteria isolated from urine cultures was E. coli (85.3%). Antibiotic resistance of E. coli isolates was detected as 4.2% to fosfomycin trometamol, 0% to imipenem, 1.6% to meropenem, 3.2% to nitrofurantoin, 16.7% to ciprofloxacin, 7.7% to gentamicin, and 4.1% to trimethoprim-sulfamethoxazole. In ESBL positive strains, these resistance ratios were identified as 8.8%, 0%, 0%, 5.9%, 47.1%, 32.4%, and 50%, respectively. Conclusion: Considering that studies conducted in previous years on E. coli strains isolated from urine specimens resulted in ratios not exceeding 1%, there has been a considerable increase in resistance ratios to fosfomycin trometamol. However, when compared to other antibiotics, fosfomycin trometamol, nitrofurantoin and gentamicin are among the antibiotics that may be preferred in the empiric treatment of community-acquired urinary tract infections. |
| first_indexed | 2024-04-10T10:26:58Z |
| format | Article |
| id | doaj.art-cbdd07390c9a4346ade1639433121381 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T10:26:58Z |
| publishDate | 2012-12-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-cbdd07390c9a4346ade16394331213812023-02-15T16:21:19ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2012-12-01174181186Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract InfectionsLaser ŞANAL0Microbiology Laboratory, Ankara Occupational Diseases Hospital, Ankara, TurkeyIntroduction: Studies indicate that as observed in most bacteria, Escherichia coli isolated from community-acquired urinary tract infections has also become resistant to most antibiotics and is increasingly becoming so as time passes. In this study, we aimed to investigate extended-spectrum beta-lactamase (ESBL) ratios in E. coli strains isolated from community-acquired urinary tract infections and the susceptibilities of these strains to fosfomycin trometamol and other antibiotics. Materials and Methods: In this study, the urinary cultures of 3257 outpatients who applied to Ankara Occupational Diseases Hospital between 01 January 2012-31 July 2012 with urinary tract complaints were examined. The urine specimens collected from patients were inoculated to 5% sheep blood agar and Eosin-Methylene-Blue (EMB) agar, and incubated at 37°C for 18-24 hours. The antibiotic susceptibility testing was performed by Kirby Bauer disk diffusion method in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. ESBL presence in E. coli strains isolated from urine samples was investigated by double disk synergy test. Results: Growth was detected in 443 (13.6%) of the 3257 patients from whom urinary cultures were requested. The most frequent bacteria isolated from urine cultures was E. coli (85.3%). Antibiotic resistance of E. coli isolates was detected as 4.2% to fosfomycin trometamol, 0% to imipenem, 1.6% to meropenem, 3.2% to nitrofurantoin, 16.7% to ciprofloxacin, 7.7% to gentamicin, and 4.1% to trimethoprim-sulfamethoxazole. In ESBL positive strains, these resistance ratios were identified as 8.8%, 0%, 0%, 5.9%, 47.1%, 32.4%, and 50%, respectively. Conclusion: Considering that studies conducted in previous years on E. coli strains isolated from urine specimens resulted in ratios not exceeding 1%, there has been a considerable increase in resistance ratios to fosfomycin trometamol. However, when compared to other antibiotics, fosfomycin trometamol, nitrofurantoin and gentamicin are among the antibiotics that may be preferred in the empiric treatment of community-acquired urinary tract infections.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2012-17-04-181-186.pdfFosfomycin trometamolExtended-spectrum beta-lactamaseUrinary tract infection |
| spellingShingle | Laser ŞANAL Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract Infections Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Fosfomycin trometamol Extended-spectrum beta-lactamase Urinary tract infection |
| title | Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract Infections |
| title_full | Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract Infections |
| title_fullStr | Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract Infections |
| title_full_unstemmed | Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract Infections |
| title_short | Extended-Spectrum Beta-Lactamase Positivity and In Vitro Activity of Fosfomycin Trometamol and Other Antibiotics in Escherichia coli Strains Isolated from Community-Acquired Urinary Tract Infections |
| title_sort | extended spectrum beta lactamase positivity and in vitro activity of fosfomycin trometamol and other antibiotics in escherichia coli strains isolated from community acquired urinary tract infections |
| topic | Fosfomycin trometamol Extended-spectrum beta-lactamase Urinary tract infection |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2012-17-04-181-186.pdf |
| work_keys_str_mv | AT lasersanal extendedspectrumbetalactamasepositivityandinvitroactivityoffosfomycintrometamolandotherantibioticsinescherichiacolistrainsisolatedfromcommunityacquiredurinarytractinfections |