Invasion and replication of Yersinia ruckeri in fish cell cultures
Abstract Background Like many members of the Enterobacteriaceae family, Yersinia ruckeri has the ability to invade non professional phagocytic cells. Intracellular location is advantageous for the bacterium because it shields it from the immune system and can help it cross epithelial membranes and g...
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1408-1 |
| _version_ | 1818145145721192448 |
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| author | Simon Menanteau-Ledouble Mark L. Lawrence Mansour El-Matbouli |
| author_facet | Simon Menanteau-Ledouble Mark L. Lawrence Mansour El-Matbouli |
| author_sort | Simon Menanteau-Ledouble |
| collection | DOAJ |
| description | Abstract Background Like many members of the Enterobacteriaceae family, Yersinia ruckeri has the ability to invade non professional phagocytic cells. Intracellular location is advantageous for the bacterium because it shields it from the immune system and can help it cross epithelial membranes and gain entry into the host. In the present manuscript, we report on our investigation regarding the mechanisms of Y. ruckeri’s invasion of host cells. Results A gentamycin assay was applied to two isolates, belonging to both the biotype 1 (ATCC 29473) and biotype 2 (A7959–11) and using several cell culture types: Atlantic Salmon Kidney, Salmon Head Kidney and, Chinook salmon embryos cells at both low and high passage numbers. Varying degrees of sensitivity to Y. ruckeri infection were found between the cell types and the biotype 1 strain was found to be more invasive than the non-motile biotype 2 isolate. Furthermore, the effect of six chemical compounds (Cytochalasin D, TAE 226, vinblastine, genistein, colchicine and, N-acetylcysteine), known to interfere with bacterial invasion strategies, were investigated. All of these compounds had a significant impact on the ability of the bacterium to invade host cells. Changes in the concentration of bacterial cells over time were investigated and the results suggested that neither isolate could survive intracellularly for sustained periods. Conclusions These results suggest that Y. ruckeri can gain entrance into host cells through several mechanisms, and might take advantage of both the actin and microtubule cytoskeletal systems. |
| first_indexed | 2024-12-11T11:58:48Z |
| format | Article |
| id | doaj.art-cbdf1fbc10b64db4b645ab13b37d33cb |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-11T11:58:48Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-cbdf1fbc10b64db4b645ab13b37d33cb2022-12-22T01:08:07ZengBMCBMC Veterinary Research1746-61482018-03-0114111110.1186/s12917-018-1408-1Invasion and replication of Yersinia ruckeri in fish cell culturesSimon Menanteau-Ledouble0Mark L. Lawrence1Mansour El-Matbouli2Clinical Division of Fish Medicine, University of Veterinary MedicineDepartment of Basic Sciences, College of Veterinary Medicine, Mississippi State UniversityClinical Division of Fish Medicine, University of Veterinary MedicineAbstract Background Like many members of the Enterobacteriaceae family, Yersinia ruckeri has the ability to invade non professional phagocytic cells. Intracellular location is advantageous for the bacterium because it shields it from the immune system and can help it cross epithelial membranes and gain entry into the host. In the present manuscript, we report on our investigation regarding the mechanisms of Y. ruckeri’s invasion of host cells. Results A gentamycin assay was applied to two isolates, belonging to both the biotype 1 (ATCC 29473) and biotype 2 (A7959–11) and using several cell culture types: Atlantic Salmon Kidney, Salmon Head Kidney and, Chinook salmon embryos cells at both low and high passage numbers. Varying degrees of sensitivity to Y. ruckeri infection were found between the cell types and the biotype 1 strain was found to be more invasive than the non-motile biotype 2 isolate. Furthermore, the effect of six chemical compounds (Cytochalasin D, TAE 226, vinblastine, genistein, colchicine and, N-acetylcysteine), known to interfere with bacterial invasion strategies, were investigated. All of these compounds had a significant impact on the ability of the bacterium to invade host cells. Changes in the concentration of bacterial cells over time were investigated and the results suggested that neither isolate could survive intracellularly for sustained periods. Conclusions These results suggest that Y. ruckeri can gain entrance into host cells through several mechanisms, and might take advantage of both the actin and microtubule cytoskeletal systems.http://link.springer.com/article/10.1186/s12917-018-1408-1Intracellular invasionGentamycin assayAtlantic Salmon kidney cellsChinook Salmon embryo cellsSalmon head kidney cells |
| spellingShingle | Simon Menanteau-Ledouble Mark L. Lawrence Mansour El-Matbouli Invasion and replication of Yersinia ruckeri in fish cell cultures BMC Veterinary Research Intracellular invasion Gentamycin assay Atlantic Salmon kidney cells Chinook Salmon embryo cells Salmon head kidney cells |
| title | Invasion and replication of Yersinia ruckeri in fish cell cultures |
| title_full | Invasion and replication of Yersinia ruckeri in fish cell cultures |
| title_fullStr | Invasion and replication of Yersinia ruckeri in fish cell cultures |
| title_full_unstemmed | Invasion and replication of Yersinia ruckeri in fish cell cultures |
| title_short | Invasion and replication of Yersinia ruckeri in fish cell cultures |
| title_sort | invasion and replication of yersinia ruckeri in fish cell cultures |
| topic | Intracellular invasion Gentamycin assay Atlantic Salmon kidney cells Chinook Salmon embryo cells Salmon head kidney cells |
| url | http://link.springer.com/article/10.1186/s12917-018-1408-1 |
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