Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells
The influence of <i>p</i>-terphenyl polyketides <b>1</b>–<b>3</b> from <i>Aspergillus candidus</i> KMM 4676 and cerebroside flavuside B (<b>4</b>) from <i>Penicillium islandicum</i> (=<i>Talaromyces islandicus</i>) a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/12/3618 |
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| author | Ekaterina A. Yurchenko Ekaterina S. Menchinskaya Evgeny A. Pislyagin Ekaterina A. Chingizova Elena V. Girich Anton N. Yurchenko Dmitry L. Aminin Valery V. Mikhailov |
| author_facet | Ekaterina A. Yurchenko Ekaterina S. Menchinskaya Evgeny A. Pislyagin Ekaterina A. Chingizova Elena V. Girich Anton N. Yurchenko Dmitry L. Aminin Valery V. Mikhailov |
| author_sort | Ekaterina A. Yurchenko |
| collection | DOAJ |
| description | The influence of <i>p</i>-terphenyl polyketides <b>1</b>–<b>3</b> from <i>Aspergillus candidus</i> KMM 4676 and cerebroside flavuside B (<b>4</b>) from <i>Penicillium islandicum</i> (=<i>Talaromyces islandicus</i>) against the effect of neurotoxins, rotenone and paraquat, on Neuro-2a cell viability by MTT and LDH release assays and intracellular ROS level, as well as DPPH radical scavenging activity, was investigated. Pre-incubation with compounds significantly diminished the ROS level in rotenone- and paraquat-treated cells. It was shown that the investigated polyketides <b>1</b>–<b>3</b> significantly increased the viability of rotenone- and paraquat-treated cells in two of the used assays but they affected only the viability of paraquat-treated cells in the LDH release assay. Flavuside B statistically increased the viability of paraquat-treated cells in both MTT and LDH release assays, however, it increased the viability of rotenone-treated cells in the LDH release assay. Structure–activity relationships for <i>p</i>-terphenyl derivatives, as well as possible mechanisms of cytoprotective action of all studied compounds, were discussed. |
| first_indexed | 2024-03-10T10:27:44Z |
| format | Article |
| id | doaj.art-cbdf3d7d24be4622b203339c7304a706 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:27:44Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cbdf3d7d24be4622b203339c7304a7062023-11-21T23:56:06ZengMDPI AGMolecules1420-30492021-06-012612361810.3390/molecules26123618Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a CellsEkaterina A. Yurchenko0Ekaterina S. Menchinskaya1Evgeny A. Pislyagin2Ekaterina A. Chingizova3Elena V. Girich4Anton N. Yurchenko5Dmitry L. Aminin6Valery V. Mikhailov7Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaLaboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaLaboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaLaboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaLaboratory of Chemistry of Microbial Metabolites, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaLaboratory of Chemistry of Microbial Metabolites, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaLaboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaLaboratory of Microbiology, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, RussiaThe influence of <i>p</i>-terphenyl polyketides <b>1</b>–<b>3</b> from <i>Aspergillus candidus</i> KMM 4676 and cerebroside flavuside B (<b>4</b>) from <i>Penicillium islandicum</i> (=<i>Talaromyces islandicus</i>) against the effect of neurotoxins, rotenone and paraquat, on Neuro-2a cell viability by MTT and LDH release assays and intracellular ROS level, as well as DPPH radical scavenging activity, was investigated. Pre-incubation with compounds significantly diminished the ROS level in rotenone- and paraquat-treated cells. It was shown that the investigated polyketides <b>1</b>–<b>3</b> significantly increased the viability of rotenone- and paraquat-treated cells in two of the used assays but they affected only the viability of paraquat-treated cells in the LDH release assay. Flavuside B statistically increased the viability of paraquat-treated cells in both MTT and LDH release assays, however, it increased the viability of rotenone-treated cells in the LDH release assay. Structure–activity relationships for <i>p</i>-terphenyl derivatives, as well as possible mechanisms of cytoprotective action of all studied compounds, were discussed.https://www.mdpi.com/1420-3049/26/12/3618marine fungisecondary metabolitescytoprotectionreactive oxygen speciesparaquatrotenone |
| spellingShingle | Ekaterina A. Yurchenko Ekaterina S. Menchinskaya Evgeny A. Pislyagin Ekaterina A. Chingizova Elena V. Girich Anton N. Yurchenko Dmitry L. Aminin Valery V. Mikhailov Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells Molecules marine fungi secondary metabolites cytoprotection reactive oxygen species paraquat rotenone |
| title | Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells |
| title_full | Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells |
| title_fullStr | Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells |
| title_full_unstemmed | Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells |
| title_short | Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells |
| title_sort | cytoprotective activity of i p i terphenyl polyketides and flavuside b from marine derived fungi against oxidative stress in neuro 2a cells |
| topic | marine fungi secondary metabolites cytoprotection reactive oxygen species paraquat rotenone |
| url | https://www.mdpi.com/1420-3049/26/12/3618 |
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