Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms
tag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an interchangeable tag reporter system.
Bibliographic Details
| Main Authors: |
Christoph Grohmann,
Charlene M. Magtoto,
Joel R. Walker,
Ngee Kiat Chua,
Anna Gabrielyan,
Mary Hall,
Simon A. Cobbold,
Stephen Mieruszynski,
Martin Brzozowski,
Daniel S. Simpson,
Hao Dong,
Bridget Dorizzi,
Annette V. Jacobsen,
Emma Morrish,
Natasha Silke,
James M. Murphy,
Joan K. Heath,
Andrea Testa,
Chiara Maniaci,
Alessio Ciulli,
Guillaume Lessene,
John Silke,
Rebecca Feltham |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Portfolio
2022-04-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-022-29670-1
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